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The map label for this gene is thiE [H]

Identifier: 154246169

GI number: 154246169

Start: 2485523

End: 2486164

Strand: Direct

Name: thiE [H]

Synonym: Xaut_2226

Alternate gene names: 154246169

Gene position: 2485523-2486164 (Clockwise)

Preceding gene: 154246168

Following gene: 154246170

Centisome position: 46.82

GC content: 72.43

Gene sequence:

>642_bases
GTGCTGCCAGAACCGCCGCTGCTGCTGATCACCGATCGCACATTGGCGCAGGGGGATCTTGCGCAGGTGGCGATGGAGGC
CTGCGTCGGCGGATGCCGCTGGATCAGCTTTCGGGAGAAGGACCTGCCGGCGGCAGAGCAGATCGCCCTGTTCGCCCATC
TGCGTGAGGCGACACGCGCCTTCCGCCCCCGCCTGACATTGCACGGCCCGGCCGATCTGGCCGCCGCCGCCGGGGCGGAC
GGGGTTCACTTGTCCGGCGGCGGCCATGCGGGCGCGGCGCGGGCGCTGCTGGGGCCCGATGCGCTGGTGGGGCTCTCGGT
CCACACTCTGGCCGAGGCGCAAGCCGCCGATGGGGCCAGCCTCGACTACATCACTGTAAGCCCGGTCTTTCTCACCGCCT
CCAAGCCCGGCCATGGCCCGGCGCTGGGGGCTTCGGGACTTGCCGCTTTCGTCGCCGCCTCTCCGGTTCCGGTGATCGCC
CTCGCCGGCATCGCCCCCGACAACGCCCGCGTGTGCCGCGATGCCGGAGCGGCCGGGCTCGCCGTCATGGGCTCGGTGAT
GCGGGCGGAGGATCCGGCTGCGGAGTTCTCGGCCCTGCTTGCGGCGTGGGATGGCAAGGGGCCGAACAGCGCGCGTGCCT
GA

Upstream 100 bases:

>100_bases
GAGGGCGGCCGCCTCGCCCGCCTGTCGGGCCGCATTCCGAAAAAGCTGCATGCGGAAGCGTCCAGCCCCGAGTTCGGGCT
GGTGGGGAGCTGAGCGCCGC

Downstream 100 bases:

>100_bases
TCCTGGCGCGGCCTTGCATGTGCGGGCCAAAAGCTGAACCCTGCCGCAAAAGGGGGAGACGGGCATGGGGCGGCAGGATT
CGGGGCAGAATTCCGCGCAG

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLPEPPLLLITDRTLAQGDLAQVAMEACVGGCRWISFREKDLPAAEQIALFAHLREATRAFRPRLTLHGPADLAAAAGAD
GVHLSGGGHAGAARALLGPDALVGLSVHTLAEAQAADGASLDYITVSPVFLTASKPGHGPALGASGLAAFVAASPVPVIA
LAGIAPDNARVCRDAGAAGLAVMGSVMRAEDPAAEFSALLAAWDGKGPNSARA

Sequences:

>Translated_213_residues
MLPEPPLLLITDRTLAQGDLAQVAMEACVGGCRWISFREKDLPAAEQIALFAHLREATRAFRPRLTLHGPADLAAAAGAD
GVHLSGGGHAGAARALLGPDALVGLSVHTLAEAQAADGASLDYITVSPVFLTASKPGHGPALGASGLAAFVAASPVPVIA
LAGIAPDNARVCRDAGAAGLAVMGSVMRAEDPAAEFSALLAAWDGKGPNSARA
>Mature_213_residues
MLPEPPLLLITDRTLAQGDLAQVAMEACVGGCRWISFREKDLPAAEQIALFAHLREATRAFRPRLTLHGPADLAAAAGAD
GVHLSGGGHAGAARALLGPDALVGLSVHTLAEAQAADGASLDYITVSPVFLTASKPGHGPALGASGLAAFVAASPVPVIA
LAGIAPDNARVCRDAGAAGLAVMGSVMRAEDPAAEFSALLAAWDGKGPNSARA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21335; Mature: 21335

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPEPPLLLITDRTLAQGDLAQVAMEACVGGCRWISFREKDLPAAEQIALFAHLREATRA
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
FRPRLTLHGPADLAAAAGADGVHLSGGGHAGAARALLGPDALVGLSVHTLAEAQAADGAS
HCCCEEEECCHHHHHHCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
LDYITVSPVFLTASKPGHGPALGASGLAAFVAASPVPVIALAGIAPDNARVCRDAGAAGL
EEEEEECEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHH
AVMGSVMRAEDPAAEFSALLAAWDGKGPNSARA
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLPEPPLLLITDRTLAQGDLAQVAMEACVGGCRWISFREKDLPAAEQIALFAHLREATRA
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
FRPRLTLHGPADLAAAAGADGVHLSGGGHAGAARALLGPDALVGLSVHTLAEAQAADGAS
HCCCEEEECCHHHHHHCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
LDYITVSPVFLTASKPGHGPALGASGLAAFVAASPVPVIALAGIAPDNARVCRDAGAAGL
EEEEEECEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHH
AVMGSVMRAEDPAAEFSALLAAWDGKGPNSARA
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA