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The map label for this gene is cysU [H]

Identifier: 154246159

GI number: 154246159

Start: 2478167

End: 2479033

Strand: Direct

Name: cysU [H]

Synonym: Xaut_2216

Alternate gene names: 154246159

Gene position: 2478167-2479033 (Clockwise)

Preceding gene: 154246158

Following gene: 154246160

Centisome position: 46.68

GC content: 67.94

Gene sequence:

>867_bases
ATGCCCCGCCTTCGTTTCGCGTTTCCGGCCGGGGCCGGCCGGGCCCCGCGCAGCGTCATTCCCGGCTTCCGCCTCTCCTT
GGGGCTGATGCTGCTCTATCTCGGCCTGATCGTGCTTCTGCCCATCGGGGCGCTGCTGCTGAAGGCGGCGGATGTGGGGT
TCTGGGGCTATATCGCCATCATCACCTCGCCACGCACCATTGCCTCGTTCCAGATCACTATCGCCACGGCGGCCGCGGCC
GCCGTGTTCAATGCAGTCTACGGGCTGGGCCTCGCCTGGGTGCTGGTGCGCTACGAGTTTCCCGGCAAGCGCCTGCTGGA
CGCCATGGTGGACGTGCCCTTCGCCCTGCCCACGGCGGTGGCGGGCCTCGCGCTCACCACGCTGTTCGCAAAGAATGGCT
GGTTCGGTGCGCCGCTGGCCCAATGGGGCATCCAGGTGGCCTATGCCCCGGCGGGCATCGTCTGCGCCATGGCCTTCACG
TCCATTCCCTTCGTGGTGCGCACCGTGCAGCCGGTGCTGGAGGACATGGAGCCCGAGCTGGAGGAGGCGGCGGCGACGCT
GGGCGCATCGGACCTCACCACCTTCCGCAAGGTGATATTCCCGACCCTGTTTCCCGCTTTCCTCGCCGGCACGTCGCTCG
CCTTCGCCCGCTCGCTGGGGGAATTCGGCGCGGTGGTGTTCATCGCCGGCAACCAGCCGTTCCGCACCGAGATCGTGGCC
CTGCTCACCTTCATCCGGCTGGAGGAATATGACTATGCCGCCGCCGCCGCCATCGCCGTGACCATGCTGGGGCTCGCCTT
CCTTATGCTGCTGGTGGTGAATGCGGTGCAGACGTGGCACCAGCGCAGTGCGCTGGGGGAGGGCTGA

Upstream 100 bases:

>100_bases
CCGTGGCGGAATGACATTTGGCGCGCGTCACGCCATAACCACCGTTCGATTGCGGCGGTGCCGCGCTCTTCCAAGCCACA
CAGCCGACAGGGCCGCGTTC

Downstream 100 bases:

>100_bases
GATGGCGGCCCCGGTCAACCGCAACCGTCGCCGGGTGGGCGATGGTGCGCGCACGCGGGCGGTGCTGCTCACCCTGGTGC
TCGCCACCACGGCGGTGGCC

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPRLRFAFPAGAGRAPRSVIPGFRLSLGLMLLYLGLIVLLPIGALLLKAADVGFWGYIAIITSPRTIASFQITIATAAAA
AVFNAVYGLGLAWVLVRYEFPGKRLLDAMVDVPFALPTAVAGLALTTLFAKNGWFGAPLAQWGIQVAYAPAGIVCAMAFT
SIPFVVRTVQPVLEDMEPELEEAAATLGASDLTTFRKVIFPTLFPAFLAGTSLAFARSLGEFGAVVFIAGNQPFRTEIVA
LLTFIRLEEYDYAAAAAIAVTMLGLAFLMLLVVNAVQTWHQRSALGEG

Sequences:

>Translated_288_residues
MPRLRFAFPAGAGRAPRSVIPGFRLSLGLMLLYLGLIVLLPIGALLLKAADVGFWGYIAIITSPRTIASFQITIATAAAA
AVFNAVYGLGLAWVLVRYEFPGKRLLDAMVDVPFALPTAVAGLALTTLFAKNGWFGAPLAQWGIQVAYAPAGIVCAMAFT
SIPFVVRTVQPVLEDMEPELEEAAATLGASDLTTFRKVIFPTLFPAFLAGTSLAFARSLGEFGAVVFIAGNQPFRTEIVA
LLTFIRLEEYDYAAAAAIAVTMLGLAFLMLLVVNAVQTWHQRSALGEG
>Mature_287_residues
PRLRFAFPAGAGRAPRSVIPGFRLSLGLMLLYLGLIVLLPIGALLLKAADVGFWGYIAIITSPRTIASFQITIATAAAAA
VFNAVYGLGLAWVLVRYEFPGKRLLDAMVDVPFALPTAVAGLALTTLFAKNGWFGAPLAQWGIQVAYAPAGIVCAMAFTS
IPFVVRTVQPVLEDMEPELEEAAATLGASDLTTFRKVIFPTLFPAFLAGTSLAFARSLGEFGAVVFIAGNQPFRTEIVAL
LTFIRLEEYDYAAAAAIAVTMLGLAFLMLLVVNAVQTWHQRSALGEG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=273, Percent_Identity=52.7472527472528, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI87082099, Length=255, Percent_Identity=32.5490196078431, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786980, Length=216, Percent_Identity=36.5740740740741, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787369, Length=202, Percent_Identity=25.2475247524752, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787368, Length=174, Percent_Identity=29.8850574712644, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30659; Mature: 30527

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLRFAFPAGAGRAPRSVIPGFRLSLGLMLLYLGLIVLLPIGALLLKAADVGFWGYIAI
CCCCEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEE
ITSPRTIASFQITIATAAAAAVFNAVYGLGLAWVLVRYEFPGKRLLDAMVDVPFALPTAV
EECCCEEHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
AGLALTTLFAKNGWFGAPLAQWGIQVAYAPAGIVCAMAFTSIPFVVRTVQPVLEDMEPEL
HHHHHHHHHHCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EEAAATLGASDLTTFRKVIFPTLFPAFLAGTSLAFARSLGEFGAVVFIAGNQPFRTEIVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
LLTFIRLEEYDYAAAAAIAVTMLGLAFLMLLVVNAVQTWHQRSALGEG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRLRFAFPAGAGRAPRSVIPGFRLSLGLMLLYLGLIVLLPIGALLLKAADVGFWGYIAI
CCCEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEE
ITSPRTIASFQITIATAAAAAVFNAVYGLGLAWVLVRYEFPGKRLLDAMVDVPFALPTAV
EECCCEEHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHH
AGLALTTLFAKNGWFGAPLAQWGIQVAYAPAGIVCAMAFTSIPFVVRTVQPVLEDMEPEL
HHHHHHHHHHCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EEAAATLGASDLTTFRKVIFPTLFPAFLAGTSLAFARSLGEFGAVVFIAGNQPFRTEIVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHH
LLTFIRLEEYDYAAAAAIAVTMLGLAFLMLLVVNAVQTWHQRSALGEG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]