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The map label for this gene is yafC [H]

Identifier: 154246137

GI number: 154246137

Start: 2453001

End: 2453924

Strand: Direct

Name: yafC [H]

Synonym: Xaut_2194

Alternate gene names: 154246137

Gene position: 2453001-2453924 (Clockwise)

Preceding gene: 154246131

Following gene: 154246138

Centisome position: 46.21

GC content: 71.65

Gene sequence:

>924_bases
GTGCGCGCGGGCGATTTCGACTGGTCTGACCTCACCTTCTTCCTCGCCGTGGCACGGGCCGGGCGGCTGACAGCGGCGGC
GGCGCGCCTGAAGGTCGAGCACTCCACCGTGAGCCGGCGCATCGGCGCGCTGGAGGAGGCGCTGGGGGCGAAGCTGTTCG
ACCGCCGGCCCCATGGCTACGGCCTCACCGCCGCCGGCGAGCGCCTGATGGCCGCCGCCGAGGGCATGGAGACCCTTGCG
CTGGCGGCGCAGGGCGAGATCGGTGGGGCGGACCTGGGCGTCGCCGGCACCGTGCGCATCGGCGCGCCGGACGGCTTCGG
CACCTTCTTCCTTGCCCCCCGCATCTCGGCGCTGGCGGACCTGCATCCGGAGCTGGACATCCAGCTCCTCGCCATGCCGC
GCCTGTTCTCCCTGTCGAAGCGCGAGGCGGACATCGCCATCTCCCTGTCGCGGCCCAAGGAGGGGCGGCTCCATGCCCGC
AAGCTCACCGACTACCGGCTGGGGCTCTATGCGTCGCGCGCCTATCTGGAGCGGCACGGGCCCATCGAGAGCCGCGCCGG
CCTCCAGCGCCACCCCTTCATCGGCTATGTGGACGACCTCATCTATGCGCCGGAGCTGGACTACGTGCCCCTGGTGGCCA
AGGACATCAGGGTGCGGCTGAAGAGCTCGAGCCTTGTCGCCCAGCTCACCGCCACCCAGGCGGGGGCGGGTGTGTGTGTC
CTGCCCTGCTTCATGGCGGACCTGTTCGCGGGCGACCGGCCGAATGCGCTGGTGCCCATCCTCCCCGGCGACGTGGGGCT
GACGCGCACCTTCTGGCTGCTCACCCACACGGATACGCGCGACCTCGCCCGCATCCGCGTGGCGGCGGATTTCATCGCCG
GCGTGGTGCAGGCGGCGCGTGGGCAATTCCTGTCCCCAAGGTAA

Upstream 100 bases:

>100_bases
CATCGATGTGCGACAATTCACACTAACGGCACTGGTCTTTGGCCGCTTGGAGGCGTGTCCGGCCACCGGATCGCATGACC
GGGATCAATGAGGGGGAGCC

Downstream 100 bases:

>100_bases
TGCGGTATCAACGAGCGTAGAGCATCGGCCACCGCCGCGTCGTGGACGGAACCGAGCCACGAACCGTCGGCCGAGAGCTC
GATGCACACCAGGACACCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRAGDFDWSDLTFFLAVARAGRLTAAAARLKVEHSTVSRRIGALEEALGAKLFDRRPHGYGLTAAGERLMAAAEGMETLA
LAAQGEIGGADLGVAGTVRIGAPDGFGTFFLAPRISALADLHPELDIQLLAMPRLFSLSKREADIAISLSRPKEGRLHAR
KLTDYRLGLYASRAYLERHGPIESRAGLQRHPFIGYVDDLIYAPELDYVPLVAKDIRVRLKSSSLVAQLTATQAGAGVCV
LPCFMADLFAGDRPNALVPILPGDVGLTRTFWLLTHTDTRDLARIRVAADFIAGVVQAARGQFLSPR

Sequences:

>Translated_307_residues
MRAGDFDWSDLTFFLAVARAGRLTAAAARLKVEHSTVSRRIGALEEALGAKLFDRRPHGYGLTAAGERLMAAAEGMETLA
LAAQGEIGGADLGVAGTVRIGAPDGFGTFFLAPRISALADLHPELDIQLLAMPRLFSLSKREADIAISLSRPKEGRLHAR
KLTDYRLGLYASRAYLERHGPIESRAGLQRHPFIGYVDDLIYAPELDYVPLVAKDIRVRLKSSSLVAQLTATQAGAGVCV
LPCFMADLFAGDRPNALVPILPGDVGLTRTFWLLTHTDTRDLARIRVAADFIAGVVQAARGQFLSPR
>Mature_307_residues
MRAGDFDWSDLTFFLAVARAGRLTAAAARLKVEHSTVSRRIGALEEALGAKLFDRRPHGYGLTAAGERLMAAAEGMETLA
LAAQGEIGGADLGVAGTVRIGAPDGFGTFFLAPRISALADLHPELDIQLLAMPRLFSLSKREADIAISLSRPKEGRLHAR
KLTDYRLGLYASRAYLERHGPIESRAGLQRHPFIGYVDDLIYAPELDYVPLVAKDIRVRLKSSSLVAQLTATQAGAGVCV
LPCFMADLFAGDRPNALVPILPGDVGLTRTFWLLTHTDTRDLARIRVAADFIAGVVQAARGQFLSPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=26, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=24.1721854304636, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789639, Length=237, Percent_Identity=25.7383966244726, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=25.8389261744966, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=30.1775147928994, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081978, Length=173, Percent_Identity=32.3699421965318, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33143; Mature: 33143

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGDFDWSDLTFFLAVARAGRLTAAAARLKVEHSTVSRRIGALEEALGAKLFDRRPHGY
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLTAAGERLMAAAEGMETLALAAQGEIGGADLGVAGTVRIGAPDGFGTFFLAPRISALAD
CCHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHH
LHPELDIQLLAMPRLFSLSKREADIAISLSRPKEGRLHARKLTDYRLGLYASRAYLERHG
CCCCCCEEEEECCHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
PIESRAGLQRHPFIGYVDDLIYAPELDYVPLVAKDIRVRLKSSSLVAQLTATQAGAGVCV
CCHHHCCCCCCCCCCHHHHHEECCCCCCCCHHHHHHHEEECCCHHEEEEHHHCCCCCCHH
LPCFMADLFAGDRPNALVPILPGDVGLTRTFWLLTHTDTRDLARIRVAADFIAGVVQAAR
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GQFLSPR
CCCCCCC
>Mature Secondary Structure
MRAGDFDWSDLTFFLAVARAGRLTAAAARLKVEHSTVSRRIGALEEALGAKLFDRRPHGY
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLTAAGERLMAAAEGMETLALAAQGEIGGADLGVAGTVRIGAPDGFGTFFLAPRISALAD
CCHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHH
LHPELDIQLLAMPRLFSLSKREADIAISLSRPKEGRLHARKLTDYRLGLYASRAYLERHG
CCCCCCEEEEECCHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
PIESRAGLQRHPFIGYVDDLIYAPELDYVPLVAKDIRVRLKSSSLVAQLTATQAGAGVCV
CCHHHCCCCCCCCCCHHHHHEECCCCCCCCHHHHHHHEEECCCHHEEEEHHHCCCCCCHH
LPCFMADLFAGDRPNALVPILPGDVGLTRTFWLLTHTDTRDLARIRVAADFIAGVVQAAR
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GQFLSPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]