Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 154245962

GI number: 154245962

Start: 2277662

End: 2278510

Strand: Direct

Name: gloB [H]

Synonym: Xaut_2019

Alternate gene names: 154245962

Gene position: 2277662-2278510 (Clockwise)

Preceding gene: 154245960

Following gene: 154245963

Centisome position: 42.9

GC content: 70.2

Gene sequence:

>849_bases
ATGCCGGCCGCGTCCGCAACCCATTCGGGCGCTGTGTCGAATGCCGTTCCGCTCGCGCTCCGGAGACCCGAGACCGTGCC
AGCCGAAATCCGCCTCGTCCCCTGCCTCTCCGACAATTATGCCGTCCTGATCCACGATCCGGTCACCGAGGCGACCGCCG
TGGTGGACGTGCCCGAGGCGACTCCCGTCATCGCGGCGCTGGAGAAGGAGGGCTGGAACCTCACCCACATCCTCGTCACC
CATCACCACGCCGACCACACCCAGGGCATCCCGGCGGTGAAGGCGCGATTCGGCGCCACCGTGGTGGGGCCGAAATCCGA
GGCCGACAAGATCCCCGGCCTCGACGTGACGGTGGTGGACGGCGATCCGGTGGCGGTGGGCTCGCTGGTAGGGCGGGTGC
TGGAGACCCCCGGCCACACCGCCGGCCCCGCCACCTACCTGTTCGAGGGCGAGAAGCTGCTGTTCGCCGGCGATACCCTG
TTCACCCTTGGCTGCGGCCGGCCGCTGGAATGCGAGCCGCCGGTGCTGTGGGACTCGCTGCAGCGCCTGCGTGCACTGCC
CGAGGACCTTCAGGTCTACAGCGGCCACGAATACACCCTGGCCAATGCCCGCTTCGCCGCCACGGTGGACCCGGACAATC
CCGCCCTCATCCGCCTTTATGGCGATGCGCAGCGCAAGCGGGACGCCGGCTCCCCCACCATTCCCTCGCGGCTGGGCGAC
GAGGTGGCCGCCAACCCCTTCCTGCGCGCGGACGATCCCGGCATGGCGGAGCGTCTCGGCATGGCGGGGGCCGAGCCGGC
CCTCGTCTTCACCGAGCTGCGGCTGCGCAAGAACGCCTTCAAGGGCTGA

Upstream 100 bases:

>100_bases
GAAACATGGATCCCCTTCTCCCGGCCGCCACCGGTCTGCCGAACACGGGGTGTGCACACCCCGCCGGCCGCTCTTCCGCT
CGGCGGCTTAATAGAGGATC

Downstream 100 bases:

>100_bases
ACCGTGGCTGATGGGCTCAACGGGCTGACCGCCGAGGAGGTGGTCGCCCTCCTCCAGCTCCAGCCGCACCCGGAGGGCGG
GCATTTCCGCGAGACCTTCC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPAASATHSGAVSNAVPLALRRPETVPAEIRLVPCLSDNYAVLIHDPVTEATAVVDVPEATPVIAALEKEGWNLTHILVT
HHHADHTQGIPAVKARFGATVVGPKSEADKIPGLDVTVVDGDPVAVGSLVGRVLETPGHTAGPATYLFEGEKLLFAGDTL
FTLGCGRPLECEPPVLWDSLQRLRALPEDLQVYSGHEYTLANARFAATVDPDNPALIRLYGDAQRKRDAGSPTIPSRLGD
EVAANPFLRADDPGMAERLGMAGAEPALVFTELRLRKNAFKG

Sequences:

>Translated_282_residues
MPAASATHSGAVSNAVPLALRRPETVPAEIRLVPCLSDNYAVLIHDPVTEATAVVDVPEATPVIAALEKEGWNLTHILVT
HHHADHTQGIPAVKARFGATVVGPKSEADKIPGLDVTVVDGDPVAVGSLVGRVLETPGHTAGPATYLFEGEKLLFAGDTL
FTLGCGRPLECEPPVLWDSLQRLRALPEDLQVYSGHEYTLANARFAATVDPDNPALIRLYGDAQRKRDAGSPTIPSRLGD
EVAANPFLRADDPGMAERLGMAGAEPALVFTELRLRKNAFKG
>Mature_281_residues
PAASATHSGAVSNAVPLALRRPETVPAEIRLVPCLSDNYAVLIHDPVTEATAVVDVPEATPVIAALEKEGWNLTHILVTH
HHADHTQGIPAVKARFGATVVGPKSEADKIPGLDVTVVDGDPVAVGSLVGRVLETPGHTAGPATYLFEGEKLLFAGDTLF
TLGCGRPLECEPPVLWDSLQRLRALPEDLQVYSGHEYTLANARFAATVDPDNPALIRLYGDAQRKRDAGSPTIPSRLGDE
VAANPFLRADDPGMAERLGMAGAEPALVFTELRLRKNAFKG

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI116642887, Length=234, Percent_Identity=40.5982905982906, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI21703352, Length=234, Percent_Identity=40.5982905982906, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI94538322, Length=272, Percent_Identity=35.6617647058824, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=35.7692307692308, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI14150041, Length=232, Percent_Identity=35.7758620689655, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI46361987, Length=191, Percent_Identity=35.6020942408377, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI41327741, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=34.2629482071713, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787158, Length=157, Percent_Identity=27.3885350318471, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=255, Percent_Identity=32.156862745098, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538952, Length=178, Percent_Identity=34.2696629213483, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320478, Length=261, Percent_Identity=29.1187739463602, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324614, Length=262, Percent_Identity=29.7709923664122, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=36.2934362934363, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=36.2934362934363, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=36.2934362934363, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI221330176, Length=167, Percent_Identity=32.3353293413174, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29932; Mature: 29801

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAASATHSGAVSNAVPLALRRPETVPAEIRLVPCLSDNYAVLIHDPVTEATAVVDVPEA
CCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCEEEEECCCC
TPVIAALEKEGWNLTHILVTHHHADHTQGIPAVKARFGATVVGPKSEADKIPGLDVTVVD
CCEEEEHHCCCCCEEEEEEEECCCCCCCCCCHHHHHCCEEEECCCCCHHCCCCCEEEEEC
GDPVAVGSLVGRVLETPGHTAGPATYLFEGEKLLFAGDTLFTLGCGRPLECEPPVLWDSL
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCHHHHHH
QRLRALPEDLQVYSGHEYTLANARFAATVDPDNPALIRLYGDAQRKRDAGSPTIPSRLGD
HHHHCCCHHHHEECCCEEEEECCEEEEEECCCCCEEEEEECCHHHHHCCCCCCCCHHHCC
EVAANPFLRADDPGMAERLGMAGAEPALVFTELRLRKNAFKG
HHHCCCCEECCCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure 
PAASATHSGAVSNAVPLALRRPETVPAEIRLVPCLSDNYAVLIHDPVTEATAVVDVPEA
CCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCEEEEECCCC
TPVIAALEKEGWNLTHILVTHHHADHTQGIPAVKARFGATVVGPKSEADKIPGLDVTVVD
CCEEEEHHCCCCCEEEEEEEECCCCCCCCCCHHHHHCCEEEECCCCCHHCCCCCEEEEEC
GDPVAVGSLVGRVLETPGHTAGPATYLFEGEKLLFAGDTLFTLGCGRPLECEPPVLWDSL
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCHHHHHH
QRLRALPEDLQVYSGHEYTLANARFAATVDPDNPALIRLYGDAQRKRDAGSPTIPSRLGD
HHHHCCCHHHHEECCCEEEEECCEEEEEECCCCCEEEEEECCHHHHHCCCCCCCCHHHCC
EVAANPFLRADDPGMAERLGMAGAEPALVFTELRLRKNAFKG
HHHCCCCEECCCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]