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The map label for this gene is dmlR [H]

Identifier: 154245815

GI number: 154245815

Start: 2108709

End: 2109641

Strand: Direct

Name: dmlR [H]

Synonym: Xaut_1871

Alternate gene names: 154245815

Gene position: 2108709-2109641 (Clockwise)

Preceding gene: 154245810

Following gene: 154245816

Centisome position: 39.72

GC content: 70.74

Gene sequence:

>933_bases
ATGATCGGCAATCTGGGTGATCTGGAAATCTTCGCGCGCATCGTGACTGCGGGCAGCCTGTCGGCGGCCGGGCGGGAGAT
GGGCCTGTCGCCGCCGGTGGTGTCCAAGAGGGTGCAGCGGCTGGAGGAGCGGCTCGGCGCGCGGCTGTTCCAGCGCACCA
CCCGCAAGGTGACCCTCACCGAGGCCGGGCAGGGTTTCTACGATCGGGTGGTGGCCATCCTCGCCTCCATCGAGGAGGCG
GAGAGCCAGCTCGCCCGCAAGTCGTCGGAGGCGCGGGGGCTGTTGCGGGTCTCGGCGCCCACCTCGTTCGGGCGGCTGCA
TATCGCGCCCCACCTGCGCCCGCTGCTCGACGCCAATCCGGGCCTCACCATCGATCTTGAGCTGTCGGATTCCTTCGTGG
ACATCGTGGGCGAGGGCTTCGACGTGGCGGTGCGCATCGCCGACCTCGATGATTCGAGCCTGGTCGCCCGCCGCCTCGCC
TCGGTCCACCGGGTGCTGTGCGCGACACCGGACTATCTGGCGCGGGTGGGCGAGCCCGGGCACATTTCCGACCTCTCGGG
CCACGTGCTGCTGGCCACCCACGGGCAGGACCCGTGGCGGCTGGAGGGGCCGGACGGGCCGGTGACGCTGCGGGTGCATT
CCCAATTGCGCACCAATTCCAACGAGGTGGTGCGCGAGGCGGTGCTGGCCTCGGTGGGCATCGCCCTGCGCTCCACCTGG
GACGTGGGGCCGGAACTGCGCTCCGGCGCGCTGAAGGTGGTGCTGCCGCGCTGGCGGGCCTCCCACCGGGTGGGACTGTT
CGCGGTCTATCCCTCGCGTCGCTTCCTGCCGCAGAAGGTGCGGGTGTTCATCGACCACCTCGCCGCGCTCTACGGCCAGG
TGCCGCCCTGGGACGAGGGCCTCGACCAGCTTCTGGAGCAGCAGGCGGCTTGA

Upstream 100 bases:

>100_bases
TCACCGGGAAAAAACGTCGCAGATTGTTTCCGTGGCGGATAAAGTCGTCCGCGCGATCCAAAACAATCGGTCCCTCAGGC
GGACGGAGGCGGGCCGGCGC

Downstream 100 bases:

>100_bases
CGCGTCCCCTTCCGCATTCCTCCGGAATGTCGCGGAACCGGTCCCTATGGTACCCGGATCGCTTCACGCGAGGCGGTGAA
AGCCATGAAAATTGCGATTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIGNLGDLEIFARIVTAGSLSAAGREMGLSPPVVSKRVQRLEERLGARLFQRTTRKVTLTEAGQGFYDRVVAILASIEEA
ESQLARKSSEARGLLRVSAPTSFGRLHIAPHLRPLLDANPGLTIDLELSDSFVDIVGEGFDVAVRIADLDDSSLVARRLA
SVHRVLCATPDYLARVGEPGHISDLSGHVLLATHGQDPWRLEGPDGPVTLRVHSQLRTNSNEVVREAVLASVGIALRSTW
DVGPELRSGALKVVLPRWRASHRVGLFAVYPSRRFLPQKVRVFIDHLAALYGQVPPWDEGLDQLLEQQAA

Sequences:

>Translated_310_residues
MIGNLGDLEIFARIVTAGSLSAAGREMGLSPPVVSKRVQRLEERLGARLFQRTTRKVTLTEAGQGFYDRVVAILASIEEA
ESQLARKSSEARGLLRVSAPTSFGRLHIAPHLRPLLDANPGLTIDLELSDSFVDIVGEGFDVAVRIADLDDSSLVARRLA
SVHRVLCATPDYLARVGEPGHISDLSGHVLLATHGQDPWRLEGPDGPVTLRVHSQLRTNSNEVVREAVLASVGIALRSTW
DVGPELRSGALKVVLPRWRASHRVGLFAVYPSRRFLPQKVRVFIDHLAALYGQVPPWDEGLDQLLEQQAA
>Mature_310_residues
MIGNLGDLEIFARIVTAGSLSAAGREMGLSPPVVSKRVQRLEERLGARLFQRTTRKVTLTEAGQGFYDRVVAILASIEEA
ESQLARKSSEARGLLRVSAPTSFGRLHIAPHLRPLLDANPGLTIDLELSDSFVDIVGEGFDVAVRIADLDDSSLVARRLA
SVHRVLCATPDYLARVGEPGHISDLSGHVLLATHGQDPWRLEGPDGPVTLRVHSQLRTNSNEVVREAVLASVGIALRSTW
DVGPELRSGALKVVLPRWRASHRVGLFAVYPSRRFLPQKVRVFIDHLAALYGQVPPWDEGLDQLLEQQAA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=36.4864864864865, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=35.3951890034364, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=33.6842105263158, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=31.2925170068027, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=32.9861111111111, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=29.96632996633, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=27.9310344827586, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=24.8148148148148, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788706, Length=254, Percent_Identity=24.8031496062992, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789173, Length=248, Percent_Identity=25.4032258064516, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790011, Length=295, Percent_Identity=23.0508474576271, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=95, Percent_Identity=33.6842105263158, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=307, Percent_Identity=26.3843648208469, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33852; Mature: 33852

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNLGDLEIFARIVTAGSLSAAGREMGLSPPVVSKRVQRLEERLGARLFQRTTRKVTLT
CCCCCHHHHHHHHHHHHCCCHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
EAGQGFYDRVVAILASIEEAESQLARKSSEARGLLRVSAPTSFGRLHIAPHLRPLLDANP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCCCHHHHCCCC
GLTIDLELSDSFVDIVGEGFDVAVRIADLDDSSLVARRLASVHRVLCATPDYLARVGEPG
CEEEEEEECCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HISDLSGHVLLATHGQDPWRLEGPDGPVTLRVHSQLRTNSNEVVREAVLASVGIALRSTW
CCCCCCCCEEEEECCCCCCEEECCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHCCC
DVGPELRSGALKVVLPRWRASHRVGLFAVYPSRRFLPQKVRVFIDHLAALYGQVPPWDEG
CCCHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LDQLLEQQAA
HHHHHHHHCC
>Mature Secondary Structure
MIGNLGDLEIFARIVTAGSLSAAGREMGLSPPVVSKRVQRLEERLGARLFQRTTRKVTLT
CCCCCHHHHHHHHHHHHCCCHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
EAGQGFYDRVVAILASIEEAESQLARKSSEARGLLRVSAPTSFGRLHIAPHLRPLLDANP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCCCHHHHCCCC
GLTIDLELSDSFVDIVGEGFDVAVRIADLDDSSLVARRLASVHRVLCATPDYLARVGEPG
CEEEEEEECCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HISDLSGHVLLATHGQDPWRLEGPDGPVTLRVHSQLRTNSNEVVREAVLASVGIALRSTW
CCCCCCCCEEEEECCCCCCEEECCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHCCC
DVGPELRSGALKVVLPRWRASHRVGLFAVYPSRRFLPQKVRVFIDHLAALYGQVPPWDEG
CCCHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
LDQLLEQQAA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]