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The map label for this gene is braE [H]

Identifier: 154245807

GI number: 154245807

Start: 2098615

End: 2099685

Strand: Direct

Name: braE [H]

Synonym: Xaut_1863

Alternate gene names: 154245807

Gene position: 2098615-2099685 (Clockwise)

Preceding gene: 154245806

Following gene: 154245808

Centisome position: 39.53

GC content: 63.77

Gene sequence:

>1071_bases
ATGGCCATCGACACCCTTCGCCCGTGCGGCGACTACCGCACCAGCTACAAGGCCGACCAGACCATCTTCGCGACCCGGCT
CACCATGCTGTTCTGCGTGGCGGGCGTGGTGCTGGCCTGCTTCGCGCCGTTCTTCTTCTCGCGCTACATCCTGAGCCTGA
TGATCCAGATCGGGTATCTGGGCATCGCCGCGCTGGGGCTGAACATCCTCGTCGGCTTTTCCGGGCAGATCTCGGTGGGC
CATGCGGTGTTCTTCGGCTTCGGCGGATTCGCCTCCGCCTTCCTCGCCGACAAGGGCGTGCCGGTGCCGCTGGCTATCGT
GCTTGCGGGACTGTGGACCACGGCGGTGGGCCTGTTGTTCGGACTGCCCGCCGCGCGGGTGAAGGGGCTCTATCTCGCCA
TCGCGACGCTGGCCGCCCAGTTCATCCTGCAGGATTTCTTCGTGCGGGCGGAATGGTTCACCGGCGGCACCCACGGCGCC
ATGGCCGCGCCCTTCACCCTGTTCGGCTATGACCTCTCCGGCGACGACCGCTATTTCTACGTGGTGCTGTTCTTCGTGGT
GGTCATGTATCTGGGCGCCGCCAACCTGATGCGCAGCCGCGACGGCCGGGCGCTGATCGCGGTGCGCGACCATTATCTCT
CGGCGGAGATGATGGGGGTGAACCTCACCAAGTATCGCACCATGGCGTTCGGCATCTCCTCCTTCTATGCCGGCATCGGC
GGCGCGCTCTATGCCCATTATGTGGGGTTCATGTCAGTGGAGGGGCTGGATATCCTGTTCTCCATCCAGTTCCTGGGCAT
GATCATCATCGGCGGGCTCGGCTCCATCATGGGCTCGCTCATGGGCACCATCTTCATGGTGCTTTTGCCCGAGGCCACCG
GCTGGCTCGCCTCCGCGCTGTCGGGTAGCGTCATCGACCAGGTGCTTCACCTGAAGGACGGCCTCACCTACATGCGCGAG
ATGCTGATCGGCCTCACCATCATCCTGTTCCTGATCTTCGAGCCGGACGGCCTCGCCCACCGCTGGCGCCTCATCAAGGC
CTATTGGAAGCTCTATCCCTTCTCTTACTGA

Upstream 100 bases:

>100_bases
CAACCTCTACGAGATCGTCCCGTTCTACGCGCTCATCATCATCCTGATGATCAAGCCCTACGGGCTGTTCGGCACCCGCG
ACATCGAGAGGGTCTGATCC

Downstream 100 bases:

>100_bases
TCAAAAGGACAGAGGCCACGCAGAACGGCCCGTCCAAAACGGAAACCCAAGGAGAGAACAAGATGAAGAAGCTCACCCTG
TCCCTCGTCTCGGCGCTGGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAIDTLRPCGDYRTSYKADQTIFATRLTMLFCVAGVVLACFAPFFFSRYILSLMIQIGYLGIAALGLNILVGFSGQISVG
HAVFFGFGGFASAFLADKGVPVPLAIVLAGLWTTAVGLLFGLPAARVKGLYLAIATLAAQFILQDFFVRAEWFTGGTHGA
MAAPFTLFGYDLSGDDRYFYVVLFFVVVMYLGAANLMRSRDGRALIAVRDHYLSAEMMGVNLTKYRTMAFGISSFYAGIG
GALYAHYVGFMSVEGLDILFSIQFLGMIIIGGLGSIMGSLMGTIFMVLLPEATGWLASALSGSVIDQVLHLKDGLTYMRE
MLIGLTIILFLIFEPDGLAHRWRLIKAYWKLYPFSY

Sequences:

>Translated_356_residues
MAIDTLRPCGDYRTSYKADQTIFATRLTMLFCVAGVVLACFAPFFFSRYILSLMIQIGYLGIAALGLNILVGFSGQISVG
HAVFFGFGGFASAFLADKGVPVPLAIVLAGLWTTAVGLLFGLPAARVKGLYLAIATLAAQFILQDFFVRAEWFTGGTHGA
MAAPFTLFGYDLSGDDRYFYVVLFFVVVMYLGAANLMRSRDGRALIAVRDHYLSAEMMGVNLTKYRTMAFGISSFYAGIG
GALYAHYVGFMSVEGLDILFSIQFLGMIIIGGLGSIMGSLMGTIFMVLLPEATGWLASALSGSVIDQVLHLKDGLTYMRE
MLIGLTIILFLIFEPDGLAHRWRLIKAYWKLYPFSY
>Mature_355_residues
AIDTLRPCGDYRTSYKADQTIFATRLTMLFCVAGVVLACFAPFFFSRYILSLMIQIGYLGIAALGLNILVGFSGQISVGH
AVFFGFGGFASAFLADKGVPVPLAIVLAGLWTTAVGLLFGLPAARVKGLYLAIATLAAQFILQDFFVRAEWFTGGTHGAM
AAPFTLFGYDLSGDDRYFYVVLFFVVVMYLGAANLMRSRDGRALIAVRDHYLSAEMMGVNLTKYRTMAFGISSFYAGIGG
ALYAHYVGFMSVEGLDILFSIQFLGMIIIGGLGSIMGSLMGTIFMVLLPEATGWLASALSGSVIDQVLHLKDGLTYMREM
LIGLTIILFLIFEPDGLAHRWRLIKAYWKLYPFSY

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=321, Percent_Identity=28.3489096573209, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38884; Mature: 38752

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDTLRPCGDYRTSYKADQTIFATRLTMLFCVAGVVLACFAPFFFSRYILSLMIQIGYL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAALGLNILVGFSGQISVGHAVFFGFGGFASAFLADKGVPVPLAIVLAGLWTTAVGLLF
HHHHHHHHHHCCCCCCEEECHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GLPAARVKGLYLAIATLAAQFILQDFFVRAEWFTGGTHGAMAAPFTLFGYDLSGDDRYFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCHHH
VVLFFVVVMYLGAANLMRSRDGRALIAVRDHYLSAEMMGVNLTKYRTMAFGISSFYAGIG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GALYAHYVGFMSVEGLDILFSIQFLGMIIIGGLGSIMGSLMGTIFMVLLPEATGWLASAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SGSVIDQVLHLKDGLTYMREMLIGLTIILFLIFEPDGLAHRWRLIKAYWKLYPFSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AIDTLRPCGDYRTSYKADQTIFATRLTMLFCVAGVVLACFAPFFFSRYILSLMIQIGYL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAALGLNILVGFSGQISVGHAVFFGFGGFASAFLADKGVPVPLAIVLAGLWTTAVGLLF
HHHHHHHHHHCCCCCCEEECHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GLPAARVKGLYLAIATLAAQFILQDFFVRAEWFTGGTHGAMAAPFTLFGYDLSGDDRYFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCHHH
VVLFFVVVMYLGAANLMRSRDGRALIAVRDHYLSAEMMGVNLTKYRTMAFGISSFYAGIG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GALYAHYVGFMSVEGLDILFSIQFLGMIIIGGLGSIMGSLMGTIFMVLLPEATGWLASAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SGSVIDQVLHLKDGLTYMREMLIGLTIILFLIFEPDGLAHRWRLIKAYWKLYPFSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]