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The map label for this gene is pcrA [H]

Identifier: 154244623

GI number: 154244623

Start: 755313

End: 757100

Strand: Reverse

Name: pcrA [H]

Synonym: Xaut_0672

Alternate gene names: 154244623

Gene position: 757100-755313 (Counterclockwise)

Preceding gene: 154244624

Following gene: 154244620

Centisome position: 14.26

GC content: 61.24

Gene sequence:

>1788_bases
ATGGAGGCGGACGGCCATCTCCTGGTCACAGGCGGGCCCGGGTCCGGCAAGACTACCATCTCCATCCTCAAGGCGGCGCA
AGTCGCCGAGCGGGACCTTCGCCCCGGTCAAAAGATCCTGTTCCTCAGCTTTGCCCGCGCCACCGTTTCCCGCGTAGTCG
AGGCGATCGAATACGAGCAGCAGATCCCGCCTGCCCAAAAGCGCCACATCGACGTCGAAACCTACCATTCCTTTTTCTGG
CGCATCCTGAAGGCCCACGGCTATCTCATCGGCCTGCCACGGCGGCTCTTCATTCTTACGCCGCCCGGCGAGGCCATTGC
GCTCTCGGCCGCCCGATCAGGTTTTCCCGCCCGGAATCTAACGGACGCGCAGAAAGCAGCGAAAAAGGTCGCGGAAGAGA
CGGAACGCGCGCGCCTTGCAACGGAAGACGGCCGTGTGTGCTTTGATCTTTATGCCCCCTATGTGGGCGACCCTCTCCAT
GGCAGCGCGCGTATTCGGCGCCTTATCGCCAGCATGTACCCTGTCATCATCCTCGATGAGTTTCAGGACACCAACGAGGC
GCAGTGGCGTGTTGTCCAGGCGCTGGGCGAAGTCTGCCGCCTGATCGCCTTGGCGGACCCGGAGCAACGAATTTACGACT
GGATCGGCGCCGATCCTGCCCGGCTCGACCATTTCCGGGAAGCCTTCAAGCCCACGGAAGTGGACCTGAGCACAGACAAT
CACCGTAGCGCCGGAACCGAGATCGCCCTGTTCGGTAATGACGTGCTCACCGGGAAATTCCGGCAGGATTCCTATAAGGG
CATCGACTTCGATGTCTTCGACCCGTTTCCCGATCCGGCGATGACCAAGCTCGTGACGACGGTTTACAAGGCGCGGAAGC
GTCTCGTGGATGCGGGCGTCGTCAATTGGTCACTGGCCGTTCTGGTGCCGACAAAGAAAATGACTCGCCTGGTATCCGAT
GCCTTCCGCCAGCCTCCTGCCGGCATGGTCGAGGTGCCCCACTCGGCGGTCATCGAACTGGAGGCCGCCATTCTCGGCGC
GGAAATCATCGCCCTTCTCATGCAGCCGGCCGACGACCGCCATTTCGGCCAGTTCATCGACCTGATGTGCAACTACTTTC
AGGGCAAGGGCGGCGATGGGCCGACACAGGGGGCACTCAAGGAGGCCGCGAACATCCGCAAGGCCTACGACGAGTGGCTG
GCCTGTCGGGCGGCAGGCAAGGCGATCCGCAAAAACAGCATGCTCGTCAACATGATTGCCGTCTACGAGCAGGCCCGTAC
CGCTGTGCTGACGGGTGACCCCGACGAGGATTGGCGCGCAGTGCGGCGGGTTCTGGAGGATGGGGGCTGCGCGCGCCTGA
AGGAGATTGGGCAAGAGGTGCGCAATCTCCGCATCCTAGAGCGCGGCACCCAACTTCGTCAGGAGCTGTCCCAAGATTGG
CGTGACAGTGGCGGCTATCGCAATGCCCTAGCTATCACGCGGCAGGCTTTTATTCAGGAGCACTTCTCGACGAACGCGAA
GCCCGAATCCGGGGTCGTCGTGATGAACATGCACAAGGCGAAGGGAAAGCAGTTCGACGAGGTCATCATCTTTGAACACT
GGCCGATCAAGCGAAAGGGGCAGCCGCCCTACAATGGCGACAGGATCGTGCGCTTCAATTCCAGGGAGCAGATCGACGAT
CAGGCGCGGCAGAATTTCCGTGTCAGCGTGACTAGAGGCAAACGGCAGACGACCATTTTGACCCCCAAATGCGATCCCTG
CGTTCTCCTTTTGGGAGATGACGAGTAG

Upstream 100 bases:

>100_bases
TGTCTTCCCATCCCTGCGGGCGGTGGCCAGGCTGCCGCACCCGCTGCGGACGAGCACCCGGAAGCGGTCGATGGAGCTGA
CTAAGGGCCAGCGGGATGTC

Downstream 100 bases:

>100_bases
GTTGAATTCCCCCACATCCCTTGATCGGTCTCTGAGGGGAGCACGTCTCAATGCAATTGAATCCGATCCAAGCTGCGTTC
GCTTCCTACGCTGCTGTTCG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MEADGHLLVTGGPGSGKTTISILKAAQVAERDLRPGQKILFLSFARATVSRVVEAIEYEQQIPPAQKRHIDVETYHSFFW
RILKAHGYLIGLPRRLFILTPPGEAIALSAARSGFPARNLTDAQKAAKKVAEETERARLATEDGRVCFDLYAPYVGDPLH
GSARIRRLIASMYPVIILDEFQDTNEAQWRVVQALGEVCRLIALADPEQRIYDWIGADPARLDHFREAFKPTEVDLSTDN
HRSAGTEIALFGNDVLTGKFRQDSYKGIDFDVFDPFPDPAMTKLVTTVYKARKRLVDAGVVNWSLAVLVPTKKMTRLVSD
AFRQPPAGMVEVPHSAVIELEAAILGAEIIALLMQPADDRHFGQFIDLMCNYFQGKGGDGPTQGALKEAANIRKAYDEWL
ACRAAGKAIRKNSMLVNMIAVYEQARTAVLTGDPDEDWRAVRRVLEDGGCARLKEIGQEVRNLRILERGTQLRQELSQDW
RDSGGYRNALAITRQAFIQEHFSTNAKPESGVVVMNMHKAKGKQFDEVIIFEHWPIKRKGQPPYNGDRIVRFNSREQIDD
QARQNFRVSVTRGKRQTTILTPKCDPCVLLLGDDE

Sequences:

>Translated_595_residues
MEADGHLLVTGGPGSGKTTISILKAAQVAERDLRPGQKILFLSFARATVSRVVEAIEYEQQIPPAQKRHIDVETYHSFFW
RILKAHGYLIGLPRRLFILTPPGEAIALSAARSGFPARNLTDAQKAAKKVAEETERARLATEDGRVCFDLYAPYVGDPLH
GSARIRRLIASMYPVIILDEFQDTNEAQWRVVQALGEVCRLIALADPEQRIYDWIGADPARLDHFREAFKPTEVDLSTDN
HRSAGTEIALFGNDVLTGKFRQDSYKGIDFDVFDPFPDPAMTKLVTTVYKARKRLVDAGVVNWSLAVLVPTKKMTRLVSD
AFRQPPAGMVEVPHSAVIELEAAILGAEIIALLMQPADDRHFGQFIDLMCNYFQGKGGDGPTQGALKEAANIRKAYDEWL
ACRAAGKAIRKNSMLVNMIAVYEQARTAVLTGDPDEDWRAVRRVLEDGGCARLKEIGQEVRNLRILERGTQLRQELSQDW
RDSGGYRNALAITRQAFIQEHFSTNAKPESGVVVMNMHKAKGKQFDEVIIFEHWPIKRKGQPPYNGDRIVRFNSREQIDD
QARQNFRVSVTRGKRQTTILTPKCDPCVLLLGDDE
>Mature_595_residues
MEADGHLLVTGGPGSGKTTISILKAAQVAERDLRPGQKILFLSFARATVSRVVEAIEYEQQIPPAQKRHIDVETYHSFFW
RILKAHGYLIGLPRRLFILTPPGEAIALSAARSGFPARNLTDAQKAAKKVAEETERARLATEDGRVCFDLYAPYVGDPLH
GSARIRRLIASMYPVIILDEFQDTNEAQWRVVQALGEVCRLIALADPEQRIYDWIGADPARLDHFREAFKPTEVDLSTDN
HRSAGTEIALFGNDVLTGKFRQDSYKGIDFDVFDPFPDPAMTKLVTTVYKARKRLVDAGVVNWSLAVLVPTKKMTRLVSD
AFRQPPAGMVEVPHSAVIELEAAILGAEIIALLMQPADDRHFGQFIDLMCNYFQGKGGDGPTQGALKEAANIRKAYDEWL
ACRAAGKAIRKNSMLVNMIAVYEQARTAVLTGDPDEDWRAVRRVLEDGGCARLKEIGQEVRNLRILERGTQLRQELSQDW
RDSGGYRNALAITRQAFIQEHFSTNAKPESGVVVMNMHKAKGKQFDEVIIFEHWPIKRKGQPPYNGDRIVRFNSREQIDD
QARQNFRVSVTRGKRQTTILTPKCDPCVLLLGDDE

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66676; Mature: 66676

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADGHLLVTGGPGSGKTTISILKAAQVAERDLRPGQKILFLSFARATVSRVVEAIEYEQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QIPPAQKRHIDVETYHSFFWRILKAHGYLIGLPRRLFILTPPGEAIALSAARSGFPARNL
HCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEHHHCCCCCCCC
TDAQKAAKKVAEETERARLATEDGRVCFDLYAPYVGDPLHGSARIRRLIASMYPVIILDE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEEC
FQDTNEAQWRVVQALGEVCRLIALADPEQRIYDWIGADPARLDHFREAFKPTEVDLSTDN
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCC
HRSAGTEIALFGNDVLTGKFRQDSYKGIDFDVFDPFPDPAMTKLVTTVYKARKRLVDAGV
CCCCCCEEEEECCCEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VNWSLAVLVPTKKMTRLVSDAFRQPPAGMVEVPHSAVIELEAAILGAEIIALLMQPADDR
EEEEEEEEECCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
HFGQFIDLMCNYFQGKGGDGPTQGALKEAANIRKAYDEWLACRAAGKAIRKNSMLVNMIA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VYEQARTAVLTGDPDEDWRAVRRVLEDGGCARLKEIGQEVRNLRILERGTQLRQELSQDW
HHHHHCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSGGYRNALAITRQAFIQEHFSTNAKPESGVVVMNMHKAKGKQFDEVIIFEHWPIKRKG
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEEEEECCCCCCCC
QPPYNGDRIVRFNSREQIDDQARQNFRVSVTRGKRQTTILTPKCDPCVLLLGDDE
CCCCCCCEEEEECCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MEADGHLLVTGGPGSGKTTISILKAAQVAERDLRPGQKILFLSFARATVSRVVEAIEYEQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QIPPAQKRHIDVETYHSFFWRILKAHGYLIGLPRRLFILTPPGEAIALSAARSGFPARNL
HCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEHHHCCCCCCCC
TDAQKAAKKVAEETERARLATEDGRVCFDLYAPYVGDPLHGSARIRRLIASMYPVIILDE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEEC
FQDTNEAQWRVVQALGEVCRLIALADPEQRIYDWIGADPARLDHFREAFKPTEVDLSTDN
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCC
HRSAGTEIALFGNDVLTGKFRQDSYKGIDFDVFDPFPDPAMTKLVTTVYKARKRLVDAGV
CCCCCCEEEEECCCEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VNWSLAVLVPTKKMTRLVSDAFRQPPAGMVEVPHSAVIELEAAILGAEIIALLMQPADDR
EEEEEEEEECCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
HFGQFIDLMCNYFQGKGGDGPTQGALKEAANIRKAYDEWLACRAAGKAIRKNSMLVNMIA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VYEQARTAVLTGDPDEDWRAVRRVLEDGGCARLKEIGQEVRNLRILERGTQLRQELSQDW
HHHHHCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSGGYRNALAITRQAFIQEHFSTNAKPESGVVVMNMHKAKGKQFDEVIIFEHWPIKRKG
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEEEEECCCCCCCC
QPPYNGDRIVRFNSREQIDDQARQNFRVSVTRGKRQTTILTPKCDPCVLLLGDDE
CCCCCCCEEEEECCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]