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The map label for this gene is alkK [H]

Identifier: 154244574

GI number: 154244574

Start: 695423

End: 697075

Strand: Reverse

Name: alkK [H]

Synonym: Xaut_0622

Alternate gene names: 154244574

Gene position: 697075-695423 (Counterclockwise)

Preceding gene: 154244575

Following gene: 154244572

Centisome position: 13.13

GC content: 66.73

Gene sequence:

>1653_bases
ATGCTCGGAACGATGATGGATGTCCCGCTGCTGTTGTCCTCGATCCTGCGGCATGCGGAGGCCTACCACAGCGACAGCGA
GGTGGTTTCGGCGACGGTGGAGGGCGGGCTTCACCGTTACACCTACGGCGAGATGTCGAAGCGCAGCCGCAAGCTCGCCA
ACGCCCTGGCGCGCCTTGGCCTGAAAACCGGTGACCGCGTCGGCACCCTGGCCTGGAACGGCTACTGCCACCTGGAGCTC
TACTACGGCGTCTCCGGCTCCGGCTTCGTGTGCCACACCATCAATCCGCGCCTGTTCCGCGAGCAGATCGCCTACATCAT
CGCCCATGCCGAAGACAGCGTGCTGTTCTTCGACCTGACCTTCCTGCCCATCGTGGAAGAACTGGCGGACCAGCTGAAAG
GGCTCAAGGCCCTGGTGGCCATGACCGACGAGGCGCACATGCCCGCCTCCCAGGTCCTTCCGGGTCTGCAGTGCTACGAG
ACGCTCATCGCCGGCGAGCCGGATACGTTCGACTGGCCGGCCTTCAGCGAGAACACCGCCTCCGGCCTGTGCTACACCTC
CGGCACCACGGGCGACCCCAAGGGCGTGCTCTATTCCCATCGCTCATGCGTGCTGCACGCCATGGCGATCGCCATGCCCG
ACGCGCTGGGGCTCTCCGCCAGCGACGTGGTCTGCCCCATCGTGCCCATGTTCCATGTGAACGCCTGGGGCCTGCCGTTC
GCCGCGCCCATGGTGGGGGCAAAGCTGGTGCTGCCCGGCGCCCACCTCGACGGCGCCAGCCTGCACGCTTTGTTCGAGGG
CGAGGGCGTCTCCTTCACCGCCGGGGTGCCCACCGTGTGGCTCGGCCTGCTCGACTGGATGGACACCCACGCCCGCAGCT
TCTCGGCGCTGAAGCGGGTGGTGATCGGCGGGTCGGCCATCCCACCCATCATGATCAGCCGGTTCCACAACATGGGCGTG
GAAGTGCGCCAGGCATGGGGCATGACGGAGACCAGTCCGGTGGGTCTCTCCGCTGCGCTCAAGCCCAAGCACAGGACGTT
GCCGCCGGAAGAGCGCCTCGCCCTCGAGTGCAAGCAGGGCCGGCCGTTGTTCGGCATGGAATTCCGCGTGGCCGGGTCCG
GCGGCAGCGAGGTGGCGCACGACGGCAGGAGCTTCGGCTCCATGCTGGTCCGCGGCCCCTGGGTGGCGCAGGCCTATTTC
AATACGCCGACGAGCGCAGCCCATGCGGACTACCCCGGCTGGTTCGATACCGGCGACGTGGTGACCATGGACCAGGAGGG
CTTCATCCAGATCGTCGACCGCACCAAGGATGTGGTGAAGTCCGGCGGCGAATGGATCTCCTCCATCGACCTCGAAAACA
TCGCCCAGGCTCATCCGGCCATCCAGGAAGCCGCCATCGTCGCGCGCCCCGATGCACGCTGGGGCGAACGGCCGGTGCTG
GTGGCGGTGCTGAAGCCGGGCGCCACCTTCTCCCGCGCCGACATGCGCGCCCATTACGAAGGCAAGATCTCCAAGTGGTG
CATGCCGGATGACGTGTTGATCGTGCCCGAGCTGCCTCACACCGCCACCGGGAAGCTCTCCAAGAAGGCGATCCGCGAGA
TCATCCTCGATGAGATCAAGCATCGCTCGCCCGACCTCGGTATCGGCTCCTGA

Upstream 100 bases:

>100_bases
ACGGCGCGCCCTCGCTGATGGCGCTCCACGCCGACGCCTTTTGACCCACCACACGCCACACGACCAACGGCCAATAGAAA
ATCAACGACGGAGGGAAGCG

Downstream 100 bases:

>100_bases
AGGGGCCGCGACGCTCCTGCCCCCATCTGCGGCCCGAGGTGGCGCTGAACGCCACCGGTCTCCTTCATCTTCACAAAGGG
GGGCATGCTCGACTGACGTG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MLGTMMDVPLLLSSILRHAEAYHSDSEVVSATVEGGLHRYTYGEMSKRSRKLANALARLGLKTGDRVGTLAWNGYCHLEL
YYGVSGSGFVCHTINPRLFREQIAYIIAHAEDSVLFFDLTFLPIVEELADQLKGLKALVAMTDEAHMPASQVLPGLQCYE
TLIAGEPDTFDWPAFSENTASGLCYTSGTTGDPKGVLYSHRSCVLHAMAIAMPDALGLSASDVVCPIVPMFHVNAWGLPF
AAPMVGAKLVLPGAHLDGASLHALFEGEGVSFTAGVPTVWLGLLDWMDTHARSFSALKRVVIGGSAIPPIMISRFHNMGV
EVRQAWGMTETSPVGLSAALKPKHRTLPPEERLALECKQGRPLFGMEFRVAGSGGSEVAHDGRSFGSMLVRGPWVAQAYF
NTPTSAAHADYPGWFDTGDVVTMDQEGFIQIVDRTKDVVKSGGEWISSIDLENIAQAHPAIQEAAIVARPDARWGERPVL
VAVLKPGATFSRADMRAHYEGKISKWCMPDDVLIVPELPHTATGKLSKKAIREIILDEIKHRSPDLGIGS

Sequences:

>Translated_550_residues
MLGTMMDVPLLLSSILRHAEAYHSDSEVVSATVEGGLHRYTYGEMSKRSRKLANALARLGLKTGDRVGTLAWNGYCHLEL
YYGVSGSGFVCHTINPRLFREQIAYIIAHAEDSVLFFDLTFLPIVEELADQLKGLKALVAMTDEAHMPASQVLPGLQCYE
TLIAGEPDTFDWPAFSENTASGLCYTSGTTGDPKGVLYSHRSCVLHAMAIAMPDALGLSASDVVCPIVPMFHVNAWGLPF
AAPMVGAKLVLPGAHLDGASLHALFEGEGVSFTAGVPTVWLGLLDWMDTHARSFSALKRVVIGGSAIPPIMISRFHNMGV
EVRQAWGMTETSPVGLSAALKPKHRTLPPEERLALECKQGRPLFGMEFRVAGSGGSEVAHDGRSFGSMLVRGPWVAQAYF
NTPTSAAHADYPGWFDTGDVVTMDQEGFIQIVDRTKDVVKSGGEWISSIDLENIAQAHPAIQEAAIVARPDARWGERPVL
VAVLKPGATFSRADMRAHYEGKISKWCMPDDVLIVPELPHTATGKLSKKAIREIILDEIKHRSPDLGIGS
>Mature_550_residues
MLGTMMDVPLLLSSILRHAEAYHSDSEVVSATVEGGLHRYTYGEMSKRSRKLANALARLGLKTGDRVGTLAWNGYCHLEL
YYGVSGSGFVCHTINPRLFREQIAYIIAHAEDSVLFFDLTFLPIVEELADQLKGLKALVAMTDEAHMPASQVLPGLQCYE
TLIAGEPDTFDWPAFSENTASGLCYTSGTTGDPKGVLYSHRSCVLHAMAIAMPDALGLSASDVVCPIVPMFHVNAWGLPF
AAPMVGAKLVLPGAHLDGASLHALFEGEGVSFTAGVPTVWLGLLDWMDTHARSFSALKRVVIGGSAIPPIMISRFHNMGV
EVRQAWGMTETSPVGLSAALKPKHRTLPPEERLALECKQGRPLFGMEFRVAGSGGSEVAHDGRSFGSMLVRGPWVAQAYF
NTPTSAAHADYPGWFDTGDVVTMDQEGFIQIVDRTKDVVKSGGEWISSIDLENIAQAHPAIQEAAIVARPDARWGERPVL
VAVLKPGATFSRADMRAHYEGKISKWCMPDDVLIVPELPHTATGKLSKKAIREIILDEIKHRSPDLGIGS

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=26.6666666666667, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=26.2172284644195, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=26.2172284644195, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI42544132, Length=518, Percent_Identity=22.972972972973, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI38505220, Length=516, Percent_Identity=23.2558139534884, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28416953, Length=533, Percent_Identity=23.4521575984991, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=23.4521575984991, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=510, Percent_Identity=21.9607843137255, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=510, Percent_Identity=21.9607843137255, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI13375727, Length=578, Percent_Identity=22.318339100346, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI8923896, Length=565, Percent_Identity=21.5929203539823, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI253970489, Length=379, Percent_Identity=23.7467018469657, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI115511026, Length=516, Percent_Identity=21.7054263565891, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI145693145, Length=525, Percent_Identity=26.8571428571429, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=24.8565965583174, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1788107, Length=520, Percent_Identity=24.2307692307692, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786810, Length=358, Percent_Identity=28.4916201117318, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789201, Length=493, Percent_Identity=22.920892494929, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788595, Length=354, Percent_Identity=24.5762711864407, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790505, Length=381, Percent_Identity=23.3595800524934, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=560, Percent_Identity=25.8928571428571, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=560, Percent_Identity=25.8928571428571, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=560, Percent_Identity=25.8928571428571, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=24.65483234714, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=555, Percent_Identity=22.7027027027027, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=25.2988047808765, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=541, Percent_Identity=25.5083179297597, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=22.3300970873786, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=26.4462809917355, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=513, Percent_Identity=21.8323586744639, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=21.6117216117216, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=20.6963249516441, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=27.5324675324675, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=378, Percent_Identity=24.3386243386243, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=546, Percent_Identity=21.0622710622711, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=564, Percent_Identity=26.063829787234, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=25.7253384912959, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=22.7364185110664, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=23.4714003944773, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653035, Length=529, Percent_Identity=20.0378071833648, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648257, Length=516, Percent_Identity=23.062015503876, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355181, Length=507, Percent_Identity=21.6962524654832, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62472339, Length=393, Percent_Identity=22.3918575063613, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24667955, Length=393, Percent_Identity=22.3918575063613, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161076582, Length=533, Percent_Identity=19.1369606003752, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24667959, Length=393, Percent_Identity=22.3918575063613, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20130357, Length=362, Percent_Identity=22.6519337016575, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356441, Length=502, Percent_Identity=21.3147410358566, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356947, Length=511, Percent_Identity=19.3737769080235, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 59566; Mature: 59566

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTMMDVPLLLSSILRHAEAYHSDSEVVSATVEGGLHRYTYGEMSKRSRKLANALARLG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHCCEEEEECHHHHHHHHHHHHHHHHHC
LKTGDRVGTLAWNGYCHLELYYGVSGSGFVCHTINPRLFREQIAYIIAHAEDSVLFFDLT
CCCCCCEEEEEECCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHEEECCCCEEEEEHH
FLPIVEELADQLKGLKALVAMTDEAHMPASQVLPGLQCYETLIAGEPDTFDWPAFSENTA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SGLCYTSGTTGDPKGVLYSHRSCVLHAMAIAMPDALGLSASDVVCPIVPMFHVNAWGLPF
CCEEEECCCCCCCCCEEECHHHHHHHHHHHHCCHHCCCCCCCCHHCCCHHEECCCCCCCH
AAPMVGAKLVLPGAHLDGASLHALFEGEGVSFTAGVPTVWLGLLDWMDTHARSFSALKRV
HHHHHCCEEEECCCCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
VIGGSAIPPIMISRFHNMGVEVRQAWGMTETSPVGLSAALKPKHRTLPPEERLALECKQG
HHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHEEEECCC
RPLFGMEFRVAGSGGSEVAHDGRSFGSMLVRGPWVAQAYFNTPTSAAHADYPGWFDTGDV
CCEECEEEEEECCCCCHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCE
VTMDQEGFIQIVDRTKDVVKSGGEWISSIDLENIAQAHPAIQEAAIVARPDARWGERPVL
EEECCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHEEEECCCCCCCCCCEE
VAVLKPGATFSRADMRAHYEGKISKWCMPDDVLIVPELPHTATGKLSKKAIREIILDEIK
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
HRSPDLGIGS
CCCCCCCCCC
>Mature Secondary Structure
MLGTMMDVPLLLSSILRHAEAYHSDSEVVSATVEGGLHRYTYGEMSKRSRKLANALARLG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHCCEEEEECHHHHHHHHHHHHHHHHHC
LKTGDRVGTLAWNGYCHLELYYGVSGSGFVCHTINPRLFREQIAYIIAHAEDSVLFFDLT
CCCCCCEEEEEECCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHEEECCCCEEEEEHH
FLPIVEELADQLKGLKALVAMTDEAHMPASQVLPGLQCYETLIAGEPDTFDWPAFSENTA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SGLCYTSGTTGDPKGVLYSHRSCVLHAMAIAMPDALGLSASDVVCPIVPMFHVNAWGLPF
CCEEEECCCCCCCCCEEECHHHHHHHHHHHHCCHHCCCCCCCCHHCCCHHEECCCCCCCH
AAPMVGAKLVLPGAHLDGASLHALFEGEGVSFTAGVPTVWLGLLDWMDTHARSFSALKRV
HHHHHCCEEEECCCCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
VIGGSAIPPIMISRFHNMGVEVRQAWGMTETSPVGLSAALKPKHRTLPPEERLALECKQG
HHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHEEEECCC
RPLFGMEFRVAGSGGSEVAHDGRSFGSMLVRGPWVAQAYFNTPTSAAHADYPGWFDTGDV
CCEECEEEEEECCCCCHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCE
VTMDQEGFIQIVDRTKDVVKSGGEWISSIDLENIAQAHPAIQEAAIVARPDARWGERPVL
EEECCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHEEEECCCCCCCCCCEE
VAVLKPGATFSRADMRAHYEGKISKWCMPDDVLIVPELPHTATGKLSKKAIREIILDEIK
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
HRSPDLGIGS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]