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The map label for this gene is pinE [H]

Identifier: 154244491

GI number: 154244491

Start: 605232

End: 605843

Strand: Reverse

Name: pinE [H]

Synonym: Xaut_0536

Alternate gene names: 154244491

Gene position: 605843-605232 (Counterclockwise)

Preceding gene: 154244497

Following gene: 154244490

Centisome position: 11.41

GC content: 68.79

Gene sequence:

>612_bases
ATGCTGATCGGCTACGCCCGCGTTTCCACCGGCGACCAAAATCTCGACCTCCAAAGGCAGGCGCTAATAGCGGCGGGATG
CCAGCGGCTCCACGAGGACAGCGGCGTTTCGGGCGCGATGGTGATCCGCCCCGGCCTGCGCCGGGCGCTGAAAACAGCGC
GCGCGGGCGACGTTTTGGTCGTTTGGCGTCTCGACCGCCTGGGGCGGTCGATCAAGGATTTGATAGACATCGTCGGGCAA
TTGCAGCAGCGCGGCATCGGCTTCCTGTCGCTGCAAGAGCAGATCGACACGACCAGCGCCGCGGGCCGCTTCTACCTGCA
CATGCTGGCGGCGCTGGCTGAATTCGAGCGCGAGCTGATCCGGGACCGGACGCGCGCGGGGTTGGACGCCGCCCGCAGCC
GCGGCGTGGTCATGGGCCGGCCGCGCAAGCTGAGCCCGGCGCAGATTGCCGATGCCCGCAACCTTATCGCCAGCGGCCGG
ACCGTCACGAGCGTTGCTGCCGCTTGCGGCGTTACCCGGACAACGCTGCGGCGGCTGCTGGGCGAACCGGCTTGCGCTAT
TTTCGCCAGCGCAAAGTCTGACCGCGCCGCGAGGCGGGACGAGGCGCGCTGA

Upstream 100 bases:

>100_bases
AATATCATTCAATAAATACAATGCATTACAAGTAGATTTTTCGTATTGCGGCCCATCTTTCGGCGATGGTCCGAAAAGGA
ACATTTATGAACCTCCCGCC

Downstream 100 bases:

>100_bases
TCCGCGCGAAGGATCGTCACCGGATGGCCGAGACCCTGCTCCGCCGAAGGCAACGCAGGGCTCGGGGCGAAGCCTAGAGC
CTGGTCCCGTAAGGGGACGC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLIGYARVSTGDQNLDLQRQALIAAGCQRLHEDSGVSGAMVIRPGLRRALKTARAGDVLVVWRLDRLGRSIKDLIDIVGQ
LQQRGIGFLSLQEQIDTTSAAGRFYLHMLAALAEFERELIRDRTRAGLDAARSRGVVMGRPRKLSPAQIADARNLIASGR
TVTSVAAACGVTRTTLRRLLGEPACAIFASAKSDRAARRDEAR

Sequences:

>Translated_203_residues
MLIGYARVSTGDQNLDLQRQALIAAGCQRLHEDSGVSGAMVIRPGLRRALKTARAGDVLVVWRLDRLGRSIKDLIDIVGQ
LQQRGIGFLSLQEQIDTTSAAGRFYLHMLAALAEFERELIRDRTRAGLDAARSRGVVMGRPRKLSPAQIADARNLIASGR
TVTSVAAACGVTRTTLRRLLGEPACAIFASAKSDRAARRDEAR
>Mature_203_residues
MLIGYARVSTGDQNLDLQRQALIAAGCQRLHEDSGVSGAMVIRPGLRRALKTARAGDVLVVWRLDRLGRSIKDLIDIVGQ
LQQRGIGFLSLQEQIDTTSAAGRFYLHMLAALAEFERELIRDRTRAGLDAARSRGVVMGRPRKLSPAQIADARNLIASGR
TVTSVAAACGVTRTTLRRLLGEPACAIFASAKSDRAARRDEAR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=53.3707865168539, Blast_Score=181, Evalue=2e-47,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=35.5191256830601, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=35.5191256830601, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22047; Mature: 22047

Theoretical pI: Translated: 11.65; Mature: 11.65

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYARVSTGDQNLDLQRQALIAAGCQRLHEDSGVSGAMVIRPGLRRALKTARAGDVLV
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEE
VWRLDRLGRSIKDLIDIVGQLQQRGIGFLSLQEQIDTTSAAGRFYLHMLAALAEFERELI
EEEHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRTRAGLDAARSRGVVMGRPRKLSPAQIADARNLIASGRTVTSVAAACGVTRTTLRRLL
HHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
GEPACAIFASAKSDRAARRDEAR
CCCHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MLIGYARVSTGDQNLDLQRQALIAAGCQRLHEDSGVSGAMVIRPGLRRALKTARAGDVLV
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEE
VWRLDRLGRSIKDLIDIVGQLQQRGIGFLSLQEQIDTTSAAGRFYLHMLAALAEFERELI
EEEHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRTRAGLDAARSRGVVMGRPRKLSPAQIADARNLIASGRTVTSVAAACGVTRTTLRRLL
HHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
GEPACAIFASAKSDRAARRDEAR
CCCHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]