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The map label for this gene is hemK [H]

Identifier: 153951215

GI number: 153951215

Start: 1132141

End: 1132956

Strand: Direct

Name: hemK [H]

Synonym: JJD26997_1284

Alternate gene names: 153951215

Gene position: 1132141-1132956 (Clockwise)

Preceding gene: 153951342

Following gene: 153952153

Centisome position: 61.36

GC content: 26.1

Gene sequence:

>816_bases
TTGACTATAAAAAATGCTCTTATAGAAACTAAATCTAACTTAAAAGGTTATGAAAATGAAGCAGTATTTATACTTTGTGA
ATATCTAAAAAAAGATAAAGCTTGGTTATTTTTAAATCAAGATATAGAAATAGATCATGAACCTTATTTTGAGCTTGTAA
GGCGTTTTAAATCTGGAGAACCTTTTGAATATATATTTGAAAAAGTGGATTTTTGGGGCTTAGAATTTAAGATAAAAAAG
GGTGTTCTAATCCCTCGCTACGATAGTGAAATTTTACTTTTTCAAATTTTAAATTTATGCAAAAAAAATACTTTTAATGG
GATTTTAGAAATAGGATTTGGAAGCGGAATTTTAAGCATTATTTTAGCCAAAGAACTTGGGTTAAAAATCACAGCCTGTG
ATATCAATCCCAAAGCCTTAGAATTAGCTTTAGAGAATGCCAAATTACACAAAGTCGATCACTTGATTGACTTTAAACTT
TGTAATTTTAAGCAAATTGAAGAAAATTATGATTTTATTTTTTCAAATCCACCTTATATTAAAAATTCATATCCTATAGA
TATTTGGGTGCAAAAAGAACCCAAAGAGGCTTTATTTGGAGGAGAAAAAGGATATGAAATTTTAGAAGAAATCATACATT
TTTCCCTTGATAAAAAAGTTAAATTTTTAGCTTGCGAATTTGGCTATGATCAAAAAGAAATTTTGGAAAAAATTTTATAT
CAAAATAATTTCATTGTTGATTTTTTCAAAGATGAACAAGATTATAATCGTGCTTTTATAGCTAAATTTGCAAATATGCG
TTATGATAAAAAATAA

Upstream 100 bases:

>100_bases
CTCTAGCAAGAGAAAATAAAGCTTTTATCAAAGCAAGTAAAATATATACATTTTTTTATCTCAGTCATCCAAGTATTAGC
GATAGAATCAAGGCTCTTTC

Downstream 100 bases:

>100_bases
AAGGAGAAACTCTTGAAAGCTGTTAATTTATTTTTACTTGCCTCCATCATAGGGGTTGAACTTATACTAGGAATAGTTGT
TGCACCAACAATTTTTTTTC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.AaoHemKP [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTIKNALIETKSNLKGYENEAVFILCEYLKKDKAWLFLNQDIEIDHEPYFELVRRFKSGEPFEYIFEKVDFWGLEFKIKK
GVLIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINPKALELALENAKLHKVDHLIDFKL
CNFKQIEENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
QNNFIVDFFKDEQDYNRAFIAKFANMRYDKK

Sequences:

>Translated_271_residues
MTIKNALIETKSNLKGYENEAVFILCEYLKKDKAWLFLNQDIEIDHEPYFELVRRFKSGEPFEYIFEKVDFWGLEFKIKK
GVLIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINPKALELALENAKLHKVDHLIDFKL
CNFKQIEENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
QNNFIVDFFKDEQDYNRAFIAKFANMRYDKK
>Mature_270_residues
TIKNALIETKSNLKGYENEAVFILCEYLKKDKAWLFLNQDIEIDHEPYFELVRRFKSGEPFEYIFEKVDFWGLEFKIKKG
VLIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINPKALELALENAKLHKVDHLIDFKLC
NFKQIEENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILYQ
NNFIVDFFKDEQDYNRAFIAKFANMRYDKK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=219, Percent_Identity=28.7671232876712, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082085, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31987; Mature: 31856

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKNALIETKSNLKGYENEAVFILCEYLKKDKAWLFLNQDIEIDHEPYFELVRRFKSGE
CCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
PFEYIFEKVDFWGLEFKIKKGVLIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSI
CHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHH
ILAKELGLKITACDINPKALELALENAKLHKVDHLIDFKLCNFKQIEENYDFIFSNPPYI
HHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCC
KNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
CCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
QNNFIVDFFKDEQDYNRAFIAKFANMRYDKK
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIKNALIETKSNLKGYENEAVFILCEYLKKDKAWLFLNQDIEIDHEPYFELVRRFKSGE
CCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
PFEYIFEKVDFWGLEFKIKKGVLIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSI
CHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHH
ILAKELGLKITACDINPKALELALENAKLHKVDHLIDFKLCNFKQIEENYDFIFSNPPYI
HHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCC
KNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
CCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
QNNFIVDFFKDEQDYNRAFIAKFANMRYDKK
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]