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The map label for this gene is ushA [H]

Identifier: 153949856

GI number: 153949856

Start: 3411153

End: 3412808

Strand: Reverse

Name: ushA [H]

Synonym: YpsIP31758_3032

Alternate gene names: 153949856

Gene position: 3412808-3411153 (Counterclockwise)

Preceding gene: 153949229

Following gene: 153948285

Centisome position: 72.25

GC content: 46.68

Gene sequence:

>1656_bases
ATGCGCTTTTCATTAACAACCACATTAGGTGCTTTGGCGGTGTCATTAGCGCTTGCTCCGGGGTGGGCGAGTGCCTGGGA
AAAAGATAAAACTTACGACATCACCATATTGCACACCAATGATCATCATGGTCACTTCTGGCAAAACGAGCAAGGTGAGT
ATGGTCTTGCTGCACAGAAAACCGTGGTGGATGAAATTCGTAAACAAGTTGCGGCAAAAGGAGGAAGCTTATTGCTACTC
TCGGGCGGTGATATTAATACTGGTGTCCCTGAATCCGATTTGCAGGATGCCGAACCCGATTTTCGCGGCATGAATTTGGT
CGGTTATGATGCGATGGCTATTGGTAACCATGAATTTGATAATCCCTTGAGTGTGCTGCGTCAACAAGAGAAGTGGGCGA
AGTTCCCGCTGCTGTCCGCCAATATCTATCAGAAAGGTACGCAACAGCGGTTATTCAAACCTTATGCTTTGTTTGATAAG
CAAGGTATCAAAATTGCCGTTCTTGGTTTGACCACAGATGACACCCCGAAGGTGAGTAACCCCGAGAATGTTGCTGATAT
AGAATTTCGGGTTCCTGCCGTTGAAGCGAAACACGTGGTTGAACAACTGCGGAAAACTGAAAAACCAGACATCATTATTG
CTGCCACACATATGGGACATTACGATGATGGTAAACACGGATCCAACGCCCCAGGTGATGTGGAAATGGCGCGTAGCTTA
CCGGCAGGCTATCTGGATATGATTGTGGGTGGGCACTCACAGAATCCAGTCTGTATGGCCAGTGAGAATCATAGGCAAGT
TGATTATGTTCCAGGTAGTCCATGCGTACCCGATCGGCAGAATGGTACCTGGATCGTGCAGGCCCATGAGTGGGGTAAAT
ATGTCGGGCGGGCTGACTTCACATTCCGTAATGGCGAATTAACATTGGTCAACTACCAGTTAATCCCGATCAATTTAAAG
AAAAAAATTGAGAAAGCTGATGGCACTCGCGAGCACGTCTTTTATACCCAAGAGATTGCTCAGGACCCCACAATGCTGAA
ATTACTGACGCCATTTGAAGAGCAAGGTAAAGCGCAACTGGGGGTTAGGATCGGCAGTGTTAATGGCAAATTAGAGGGTG
ATCGCAGTAAGGTCCGTTTTGAGCAAACCAATATGGCGCGTTTATTATTAGCCTCTCAAATAGAACGCTCAGGAGCTGAC
TTTGCCGTAATGAGTGGCGGGGGAGTTCGTGACTCTATCGGTGCAGGTGATATCACTTATAAAGATGTGCTCAAGGTTCA
GCCCTTTGGTAATACGCTGGTTTACGTTGATATGAAAGGTAGCGAGGTTGAGAAATACCTGGCGGTGGTAGCCAATAAGC
AGCCAGATTCAGGGGCTTATGCGCAATTTGCCAACGTCAGTCTGGTCGCTGATGGTCAGGGTGTTAGCAATGTGAAAATC
CAAGGTGAACCGTTAGATCCTAATAAAACGTACCGTATGGCTACGCTCAATTTCAATGCACTGGGCGGGGATGGTTATCC
AAGAATCGACAATTCACCTAGTTATGTTAATACCGGATTTATCGATGCTGAAGTACTAAAAGAATATATTGAAAAACACT
CGCCACTGGATGCTGAGCAGTATCAGCCGAAGGGGGAGATCGTTTATAACCAGTAG

Upstream 100 bases:

>100_bases
GTTTAGATCTTTCGTATTCTTACTCATAAAACCAGTCCTTATCCGAGCAATTTTTAGCGACAGACAGTATTTTTAACGAC
AGACAATATGGAGTTTTACC

Downstream 100 bases:

>100_bases
GCTGTTTATTAAAGATGTACTTATTTATAATCTGCCGCAAACCAAGCTGTAAAAATTAAGGGTATCGCCCAGTGGCACTG
AGTGGCTGGGCTATACCGGT

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MRFSLTTTLGALAVSLALAPGWASAWEKDKTYDITILHTNDHHGHFWQNEQGEYGLAAQKTVVDEIRKQVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWAKFPLLSANIYQKGTQQRLFKPYALFDK
QGIKIAVLGLTTDDTPKVSNPENVADIEFRVPAVEAKHVVEQLRKTEKPDIIIAATHMGHYDDGKHGSNAPGDVEMARSL
PAGYLDMIVGGHSQNPVCMASENHRQVDYVPGSPCVPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLVNYQLIPINLK
KKIEKADGTREHVFYTQEIAQDPTMLKLLTPFEEQGKAQLGVRIGSVNGKLEGDRSKVRFEQTNMARLLLASQIERSGAD
FAVMSGGGVRDSIGAGDITYKDVLKVQPFGNTLVYVDMKGSEVEKYLAVVANKQPDSGAYAQFANVSLVADGQGVSNVKI
QGEPLDPNKTYRMATLNFNALGGDGYPRIDNSPSYVNTGFIDAEVLKEYIEKHSPLDAEQYQPKGEIVYNQ

Sequences:

>Translated_551_residues
MRFSLTTTLGALAVSLALAPGWASAWEKDKTYDITILHTNDHHGHFWQNEQGEYGLAAQKTVVDEIRKQVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWAKFPLLSANIYQKGTQQRLFKPYALFDK
QGIKIAVLGLTTDDTPKVSNPENVADIEFRVPAVEAKHVVEQLRKTEKPDIIIAATHMGHYDDGKHGSNAPGDVEMARSL
PAGYLDMIVGGHSQNPVCMASENHRQVDYVPGSPCVPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLVNYQLIPINLK
KKIEKADGTREHVFYTQEIAQDPTMLKLLTPFEEQGKAQLGVRIGSVNGKLEGDRSKVRFEQTNMARLLLASQIERSGAD
FAVMSGGGVRDSIGAGDITYKDVLKVQPFGNTLVYVDMKGSEVEKYLAVVANKQPDSGAYAQFANVSLVADGQGVSNVKI
QGEPLDPNKTYRMATLNFNALGGDGYPRIDNSPSYVNTGFIDAEVLKEYIEKHSPLDAEQYQPKGEIVYNQ
>Mature_551_residues
MRFSLTTTLGALAVSLALAPGWASAWEKDKTYDITILHTNDHHGHFWQNEQGEYGLAAQKTVVDEIRKQVAAKGGSLLLL
SGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLSVLRQQEKWAKFPLLSANIYQKGTQQRLFKPYALFDK
QGIKIAVLGLTTDDTPKVSNPENVADIEFRVPAVEAKHVVEQLRKTEKPDIIIAATHMGHYDDGKHGSNAPGDVEMARSL
PAGYLDMIVGGHSQNPVCMASENHRQVDYVPGSPCVPDRQNGTWIVQAHEWGKYVGRADFTFRNGELTLVNYQLIPINLK
KKIEKADGTREHVFYTQEIAQDPTMLKLLTPFEEQGKAQLGVRIGSVNGKLEGDRSKVRFEQTNMARLLLASQIERSGAD
FAVMSGGGVRDSIGAGDITYKDVLKVQPFGNTLVYVDMKGSEVEKYLAVVANKQPDSGAYAQFANVSLVADGQGVSNVKI
QGEPLDPNKTYRMATLNFNALGGDGYPRIDNSPSYVNTGFIDAEVLKEYIEKHSPLDAEQYQPKGEIVYNQ

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=583, Percent_Identity=25.3859348198971, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1786687, Length=539, Percent_Identity=74.582560296846, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=591, Percent_Identity=22.673434856176, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922446, Length=551, Percent_Identity=25.7713248638838, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24654424, Length=551, Percent_Identity=25.7713248638838, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28573524, Length=560, Percent_Identity=24.1071428571429, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922444, Length=540, Percent_Identity=23.7037037037037, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641187, Length=576, Percent_Identity=23.6111111111111, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221329836, Length=571, Percent_Identity=22.0665499124343, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60657; Mature: 60657

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSLTTTLGALAVSLALAPGWASAWEKDKTYDITILHTNDHHGHFWQNEQGEYGLAAQK
CEEEEHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TVVDEIRKQVAAKGGSLLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWAKFPLLSANIYQKGTQQRLFKPYALFDKQGIKIAVLGLTTDDTPKVSN
CHHHHHHHHHHHHCCCEEECHHHHCCCHHHHCCCHHHCCCCCCEEEEEEECCCCCCCCCC
PENVADIEFRVPAVEAKHVVEQLRKTEKPDIIIAATHMGHYDDGKHGSNAPGDVEMARSL
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCC
PAGYLDMIVGGHSQNPVCMASENHRQVDYVPGSPCVPDRQNGTWIVQAHEWGKYVGRADF
CCCCCHHEECCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
TFRNGELTLVNYQLIPINLKKKIEKADGTREHVFYTQEIAQDPTMLKLLTPFEEQGKAQL
EEECCEEEEEEEEEEEECHHHHHHHCCCCHHEEEEEHHHHCCCCEEEEECCHHHCCCEEE
GVRIGSVNGKLEGDRSKVRFEQTNMARLLLASQIERSGADFAVMSGGGVRDSIGAGDITY
EEEEECCCCEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
KDVLKVQPFGNTLVYVDMKGSEVEKYLAVVANKQPDSGAYAQFANVSLVADGQGVSNVKI
HHEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCEEEE
QGEPLDPNKTYRMATLNFNALGGDGYPRIDNSPSYVNTGFIDAEVLKEYIEKHSPLDAEQ
ECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHH
YQPKGEIVYNQ
CCCCCCEEECC
>Mature Secondary Structure
MRFSLTTTLGALAVSLALAPGWASAWEKDKTYDITILHTNDHHGHFWQNEQGEYGLAAQK
CEEEEHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHH
TVVDEIRKQVAAKGGSLLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLSVLRQQEKWAKFPLLSANIYQKGTQQRLFKPYALFDKQGIKIAVLGLTTDDTPKVSN
CHHHHHHHHHHHHCCCEEECHHHHCCCHHHHCCCHHHCCCCCCEEEEEEECCCCCCCCCC
PENVADIEFRVPAVEAKHVVEQLRKTEKPDIIIAATHMGHYDDGKHGSNAPGDVEMARSL
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCC
PAGYLDMIVGGHSQNPVCMASENHRQVDYVPGSPCVPDRQNGTWIVQAHEWGKYVGRADF
CCCCCHHEECCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
TFRNGELTLVNYQLIPINLKKKIEKADGTREHVFYTQEIAQDPTMLKLLTPFEEQGKAQL
EEECCEEEEEEEEEEEECHHHHHHHCCCCHHEEEEEHHHHCCCCEEEEECCHHHCCCEEE
GVRIGSVNGKLEGDRSKVRFEQTNMARLLLASQIERSGADFAVMSGGGVRDSIGAGDITY
EEEEECCCCEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
KDVLKVQPFGNTLVYVDMKGSEVEKYLAVVANKQPDSGAYAQFANVSLVADGQGVSNVKI
HHEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCEEEE
QGEPLDPNKTYRMATLNFNALGGDGYPRIDNSPSYVNTGFIDAEVLKEYIEKHSPLDAEQ
ECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHHH
YQPKGEIVYNQ
CCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA