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The map label for this gene is amiD [H]

Identifier: 153949509

GI number: 153949509

Start: 1957131

End: 1957982

Strand: Direct

Name: amiD [H]

Synonym: YpsIP31758_1684

Alternate gene names: 153949509

Gene position: 1957131-1957982 (Clockwise)

Preceding gene: 153948128

Following gene: 153948396

Centisome position: 41.44

GC content: 48.36

Gene sequence:

>852_bases
ATGAGGAAGCTATTGAGTGGGGGGCTATTATTGTTGCTGGCCGGCTGCAGTAGTTCAGATCATCGCAGCTCGAATGAGTT
GATCGACCGTGGCGCATATCAAATTGATACCCATTATCCCTCTGTTGCAAAAAATGAGCGGGTACGGTTTTTAGTCCTTC
ATTACACTGCAGTCGATGACGCCGAGTCACTAAGATTGCTCACTCAGGGTGAGGTGAGTGCCCATTATTTAATCCCTACT
CACCCAAAGAAAGCTGGTGGAAAAGCCATTGCTTTGCAATTAGTGCCAGAGGCGCAGAGAGCCTGGCATGCGGGTGTCAG
CTCTTGGCAGGGGCGTAACAACCTCAACGACACCTCGATTGGTATCGAAATCGTCAACCTAGGCTTCACGGAAAAGATGC
TAGGAAGAACCTGGTATCCCTACAACGAATCACAGATTGAATTGATAGAGCAGCTCACCAAAGACATTGTACAACGTTAC
AATATTTCTCCGTCTGATGTTGTGGCCCACAGTGATATTGCTCCACTGAGAAAATCTGATCCCGGCCCATTATTCCCATG
GAAGCGCTTGGCCGAGAAGGGCGTAGGTGCCTGGCCAGATGATGCGACAGTGGCAAAATATATTGGTGGAAGAGATAAGA
AGGGCGCTGCTTCAGTAGCCGTCATTCAGCAGGCATTAGCGGCTTATGGCTACAAAATCCCCCAGAATGGCCAGTTGGAT
ACTGAAACTCGCCAAGTGATCAAAGCGTTCCAGATGCATTTCCGGCCTCAGGATTTCAGTGGTGTGCCTGATGTTGAAAC
TGAAGCCATTGCATTAGCATTAGTTGAAAAATATCGTACCCTTAGCACTTGA

Upstream 100 bases:

>100_bases
TTTGGCCTTTTTACTGATTATTGATAAGTATTGTTTATAATTTGTTTAATTTTTTTAGGTCATGATAACCGCGATTAATT
AAATTATAGGGAGATAAATA

Downstream 100 bases:

>100_bases
GGCTGCCGGGGGGAGTTGTGTCCACTCATCCGAATCACTGATTTGAGTAAATAATTCGTCTACTCAAATAGCTAACACAG
CGTGGTGAAGGGCAGTTTAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRKLLSGGLLLLLAGCSSSDHRSSNELIDRGAYQIDTHYPSVAKNERVRFLVLHYTAVDDAESLRLLTQGEVSAHYLIPT
HPKKAGGKAIALQLVPEAQRAWHAGVSSWQGRNNLNDTSIGIEIVNLGFTEKMLGRTWYPYNESQIELIEQLTKDIVQRY
NISPSDVVAHSDIAPLRKSDPGPLFPWKRLAEKGVGAWPDDATVAKYIGGRDKKGAASVAVIQQALAAYGYKIPQNGQLD
TETRQVIKAFQMHFRPQDFSGVPDVETEAIALALVEKYRTLST

Sequences:

>Translated_283_residues
MRKLLSGGLLLLLAGCSSSDHRSSNELIDRGAYQIDTHYPSVAKNERVRFLVLHYTAVDDAESLRLLTQGEVSAHYLIPT
HPKKAGGKAIALQLVPEAQRAWHAGVSSWQGRNNLNDTSIGIEIVNLGFTEKMLGRTWYPYNESQIELIEQLTKDIVQRY
NISPSDVVAHSDIAPLRKSDPGPLFPWKRLAEKGVGAWPDDATVAKYIGGRDKKGAASVAVIQQALAAYGYKIPQNGQLD
TETRQVIKAFQMHFRPQDFSGVPDVETEAIALALVEKYRTLST
>Mature_283_residues
MRKLLSGGLLLLLAGCSSSDHRSSNELIDRGAYQIDTHYPSVAKNERVRFLVLHYTAVDDAESLRLLTQGEVSAHYLIPT
HPKKAGGKAIALQLVPEAQRAWHAGVSSWQGRNNLNDTSIGIEIVNLGFTEKMLGRTWYPYNESQIELIEQLTKDIVQRY
NISPSDVVAHSDIAPLRKSDPGPLFPWKRLAEKGVGAWPDDATVAKYIGGRDKKGAASVAVIQQALAAYGYKIPQNGQLD
TETRQVIKAFQMHFRPQDFSGVPDVETEAIALALVEKYRTLST

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=264, Percent_Identity=46.5909090909091, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1786300, Length=119, Percent_Identity=39.4957983193277, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31292; Mature: 31292

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLSGGLLLLLAGCSSSDHRSSNELIDRGAYQIDTHYPSVAKNERVRFLVLHYTAVDD
CCCHHHCCEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCC
AESLRLLTQGEVSAHYLIPTHPKKAGGKAIALQLVPEAQRAWHAGVSSWQGRNNLNDTSI
HHHHEEEECCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCHHCCCCCCCCCCEE
GIEIVNLGFTEKMLGRTWYPYNESQIELIEQLTKDIVQRYNISPSDVVAHSDIAPLRKSD
EEEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCC
PGPLFPWKRLAEKGVGAWPDDATVAKYIGGRDKKGAASVAVIQQALAAYGYKIPQNGQLD
CCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TETRQVIKAFQMHFRPQDFSGVPDVETEAIALALVEKYRTLST
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLLSGGLLLLLAGCSSSDHRSSNELIDRGAYQIDTHYPSVAKNERVRFLVLHYTAVDD
CCCHHHCCEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCC
AESLRLLTQGEVSAHYLIPTHPKKAGGKAIALQLVPEAQRAWHAGVSSWQGRNNLNDTSI
HHHHEEEECCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCHHCCCCCCCCCCEE
GIEIVNLGFTEKMLGRTWYPYNESQIELIEQLTKDIVQRYNISPSDVVAHSDIAPLRKSD
EEEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCC
PGPLFPWKRLAEKGVGAWPDDATVAKYIGGRDKKGAASVAVIQQALAAYGYKIPQNGQLD
CCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TETRQVIKAFQMHFRPQDFSGVPDVETEAIALALVEKYRTLST
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]