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The map label for this gene is panE

Identifier: 153946955

GI number: 153946955

Start: 3497424

End: 3498335

Strand: Direct

Name: panE

Synonym: YpsIP31758_3106

Alternate gene names: 153946955

Gene position: 3497424-3498335 (Clockwise)

Preceding gene: 153947421

Following gene: 153947464

Centisome position: 74.05

GC content: 46.49

Gene sequence:

>912_bases
ATGAAAATTACTGTGCTTGGTTGTGGAGCTCTCGGGCAGTTATGGCTTTCAATGTTGCATCAGCAAGACCATGATGTGCA
GGGCTGGCTTCGTGTGCCACAGCCCTTTTGTTCAGTTAATGTGATCATGTTAAACGGTGAGTCGTTTAATCGAAATCTTA
CAACGAATGACCCAAAGCATTTGTTGCAAAGTGAATTATTACTGGTGTGTTTAAAAGCCTGGCAAGTTTCCAGTGCGGTT
ACCGCCTTGCTGCCTAAACTAAACCCAGAATGTAAAATATTACTGTTACATAACGGCATGGGGACACAGGAAGAGTTACC
ACTGAATGAACATGTTTTTCTGCACGGCGTGACCACTCATGCCGCACGCCGGGACGGCAATACGATTATTCATGTCGCCA
GTGGCATGACCCATATTGGCCCAACTTCATCGGTTATAATAGATGACAACCATCTGGCCGATACACTCCATCAGGCGCTA
CCAGATGTCGCTTGGCATAATGATATCTCAGCTGCCTGTTGGCAAAAACTGGCGGTCAATTGTGTCATCAATCCTCTTAC
CGGTCTGTATAACTGCCGCAATGGTGATGTACAACGTTATCCTGAGCTGATTGAACGTTTATGTGCTGAAGTTGCCAGCG
TCATGGAGATGGAGGGGTATCACACCTCCACCGAAAGTCTGCTCAGCTATGTCAACAATGTTATCCGGAGCACTGCCGAT
AATACCTCATCACTGTTACAGGATTTACGTAGCCAACGGCATACCGAAATCGACTATATAACCGGTTATTTACTGCGGCG
CGCCCGTAGTCATGGTATGGCATTACCAGAAAATGCTCGACTGTATGAATTAATCAAACGTAAGGAAAGTGATTATGAGC
GTATCGGCGCTGGTTTGCCTGGCTCCTGGTAG

Upstream 100 bases:

>100_bases
TTTATCGCTGACCCTGCGTTTAGTGAAAAAAAAGTCTATAATCAATGCATAACGCTTAAAAATAAGCAGTGTATTTTCTT
TTCATACCAGGGGGATCCCA

Downstream 100 bases:

>100_bases
CGAGGAAACCGAGGCAGTCACAACTATTGATATTCTGGTGCGCGCAGGCATCAATGTCACAACCGCCAGTGTCGCGGGTG
ATGGCGCACTGGAGATTATC

Product: 2-dehydropantoate 2-reductase

Products: NA

Alternate protein names: Ketopantoate reductase; KPA reductase; KPR

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKITVLGCGALGQLWLSMLHQQDHDVQGWLRVPQPFCSVNVIMLNGESFNRNLTTNDPKHLLQSELLLVCLKAWQVSSAV
TALLPKLNPECKILLLHNGMGTQEELPLNEHVFLHGVTTHAARRDGNTIIHVASGMTHIGPTSSVIIDDNHLADTLHQAL
PDVAWHNDISAACWQKLAVNCVINPLTGLYNCRNGDVQRYPELIERLCAEVASVMEMEGYHTSTESLLSYVNNVIRSTAD
NTSSLLQDLRSQRHTEIDYITGYLLRRARSHGMALPENARLYELIKRKESDYERIGAGLPGSW

Sequences:

>Translated_303_residues
MKITVLGCGALGQLWLSMLHQQDHDVQGWLRVPQPFCSVNVIMLNGESFNRNLTTNDPKHLLQSELLLVCLKAWQVSSAV
TALLPKLNPECKILLLHNGMGTQEELPLNEHVFLHGVTTHAARRDGNTIIHVASGMTHIGPTSSVIIDDNHLADTLHQAL
PDVAWHNDISAACWQKLAVNCVINPLTGLYNCRNGDVQRYPELIERLCAEVASVMEMEGYHTSTESLLSYVNNVIRSTAD
NTSSLLQDLRSQRHTEIDYITGYLLRRARSHGMALPENARLYELIKRKESDYERIGAGLPGSW
>Mature_303_residues
MKITVLGCGALGQLWLSMLHQQDHDVQGWLRVPQPFCSVNVIMLNGESFNRNLTTNDPKHLLQSELLLVCLKAWQVSSAV
TALLPKLNPECKILLLHNGMGTQEELPLNEHVFLHGVTTHAARRDGNTIIHVASGMTHIGPTSSVIIDDNHLADTLHQAL
PDVAWHNDISAACWQKLAVNCVINPLTGLYNCRNGDVQRYPELIERLCAEVASVMEMEGYHTSTESLLSYVNNVIRSTAD
NTSSLLQDLRSQRHTEIDYITGYLLRRARSHGMALPENARLYELIKRKESDYERIGAGLPGSW

Specific function: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family

Homologues:

Organism=Escherichia coli, GI1786627, Length=303, Percent_Identity=63.036303630363, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PANE_YERPE (Q8ZC51)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0385
- RefSeq:   NP_668344.1
- RefSeq:   NP_992148.1
- RefSeq:   YP_002348076.1
- ProteinModelPortal:   Q8ZC51
- SMR:   Q8ZC51
- IntAct:   Q8ZC51
- GeneID:   1145961
- GeneID:   1175986
- GeneID:   2764026
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3171
- KEGG:   ypk:y1014
- KEGG:   ypm:YP_0760
- HOGENOM:   HBG668370
- OMA:   QCHILLL
- ProtClustDB:   PRK06522
- BioCyc:   YPES187410:Y1014-MONOMER
- BioCyc:   YPES214092:YPO3171-MONOMER
- BRENDA:   1.1.1.169
- GO:   GO:0005737
- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR21708:SF21
- TIGRFAMs:   TIGR00745

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C; SSF48179 6DGDH_C_like

EC number: =1.1.1.169

Molecular weight: Translated: 33844; Mature: 33844

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 31-31 BINDING 98-98 BINDING 98-98 BINDING 122-122 BINDING 180-180 BINDING 184-184 BINDING 194-194 BINDING 241-241 BINDING 244-244 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVLGCGALGQLWLSMLHQQDHDVQGWLRVPQPFCSVNVIMLNGESFNRNLTTNDPKH
CEEEEEECCHHHHHHHHHHHCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCCCCHHH
LLQSELLLVCLKAWQVSSAVTALLPKLNPECKILLLHNGMGTQEELPLNEHVFLHGVTTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEHHHH
AARRDGNTIIHVASGMTHIGPTSSVIIDDNHLADTLHQALPDVAWHNDISAACWQKLAVN
HHHCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
CVINPLTGLYNCRNGDVQRYPELIERLCAEVASVMEMEGYHTSTESLLSYVNNVIRSTAD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
NTSSLLQDLRSQRHTEIDYITGYLLRRARSHGMALPENARLYELIKRKESDYERIGAGLP
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCC
GSW
CCC
>Mature Secondary Structure
MKITVLGCGALGQLWLSMLHQQDHDVQGWLRVPQPFCSVNVIMLNGESFNRNLTTNDPKH
CEEEEEECCHHHHHHHHHHHCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCCCCHHH
LLQSELLLVCLKAWQVSSAVTALLPKLNPECKILLLHNGMGTQEELPLNEHVFLHGVTTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEHHHH
AARRDGNTIIHVASGMTHIGPTSSVIIDDNHLADTLHQALPDVAWHNDISAACWQKLAVN
HHHCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHH
CVINPLTGLYNCRNGDVQRYPELIERLCAEVASVMEMEGYHTSTESLLSYVNNVIRSTAD
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
NTSSLLQDLRSQRHTEIDYITGYLLRRARSHGMALPENARLYELIKRKESDYERIGAGLP
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCC
GSW
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430