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The map label for this gene is spaR [H]

Identifier: 153937086

GI number: 153937086

Start: 1685890

End: 1686549

Strand: Direct

Name: spaR [H]

Synonym: CLC_1615

Alternate gene names: 153937086

Gene position: 1685890-1686549 (Clockwise)

Preceding gene: 153936123

Following gene: 153934620

Centisome position: 44.83

GC content: 32.58

Gene sequence:

>660_bases
ATGTCGAAAATACTTGTAATTGATGATGAAGAAGATATTTTAGCCCTTGTTAAAAATGTTCTTTCCAAGGATAATCACTT
AGTAACTACAGTAAAGGAAGCAAAAGATATTCCGTTAAATGATTTTGTAAAATTTGATCTGATTTTACTAGATGTAATGA
TGCCTGATATTGATGGCTTCACTTTGTGTAAGGAAATTAGAGAGATGGTAGACTGTCCAATTCTTTTTTTAACAGCAAAA
ACTATGGAAAGTGACATTATGTTTGGATTAGGAATTGGTGGAGATGATTATATAACGAAACCTTTTGGAGTAGGAGAACT
AAGAGCTAGAGTAAATGCTCATCTACGCAGGGAACATCGTGAAAAACGTCATATTTTTGTAGCTTCAGATATTATATTTA
ATCTATCCAGTAAGGAAATTAAAATAGGCGATGTAAAGGTGGCCTTCACAAAGGGAGAATATGCTATATGTGAATACCTT
GCAAGAAATTCAGGACAGGTGTTTTCTAAAGAACAAATATATGAAGCGGTTTATGGGTATGATGGAGAAAGTGATAGCTC
TGCTATTACAGAGCATATTAAAAATATTAGAGCTAAGTTAAAAGTTTTTGATATATCACCAATAGAAACAGTATGGGGGA
TTGGTTACAAATGGGTATAA

Upstream 100 bases:

>100_bases
AAATAGAAAATTTCAGTAATTATCAATAACAAGCTGTATTTTTTTCTAATAGGGTAGTTTATAATTAAAAATGAAGATAT
TATAAAAATAGGAGGATATT

Downstream 100 bases:

>100_bases
ATAAAAAACAGGTCACATTAAAAACTGTTTTTTTTAGATATCTATTAGCATTAGCTCTTGCTTTTTTTGTAGCAATAATA
ATTGAAATAATTATAGCAAG

Product: putative antibiotic transporter response regulator

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSKILVIDDEEDILALVKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREMVDCPILFLTAK
TMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVAFTKGEYAICEYL
ARNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDISPIETVWGIGYKWV

Sequences:

>Translated_219_residues
MSKILVIDDEEDILALVKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREMVDCPILFLTAK
TMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVAFTKGEYAICEYL
ARNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDISPIETVWGIGYKWV
>Mature_218_residues
SKILVIDDEEDILALVKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREMVDCPILFLTAKT
MESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVAFTKGEYAICEYLA
RNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDISPIETVWGIGYKWV

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=35.5855855855856, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=31.6742081447964, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=32.0175438596491, Blast_Score=116, Evalue=9e-28,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=30.3571428571429, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=30.9417040358744, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=29.4117647058824, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1786784, Length=177, Percent_Identity=33.8983050847458, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=31.1926605504587, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=32.3008849557522, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787375, Length=218, Percent_Identity=32.1100917431193, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=24.1228070175439, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787229, Length=208, Percent_Identity=25.4807692307692, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=23.4042553191489, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24841; Mature: 24710

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKILVIDDEEDILALVKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGF
CCEEEEEECCHHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHH
TLCKEIREMVDCPILFLTAKTMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKRHIFVASDIIFNLSSKEIKIGDVKVAFTKGEYAICEYLARNSGQVFSKEQIYEAVYGY
HHHEEEEEHHEEEECCCCEEEECEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
DGESDSSAITEHIKNIRAKLKVFDISPIETVWGIGYKWV
CCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHCCCCCCC
>Mature Secondary Structure 
SKILVIDDEEDILALVKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGF
CEEEEEECCHHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHH
TLCKEIREMVDCPILFLTAKTMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKRHIFVASDIIFNLSSKEIKIGDVKVAFTKGEYAICEYLARNSGQVFSKEQIYEAVYGY
HHHEEEEEHHEEEECCCCEEEECEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
DGESDSSAITEHIKNIRAKLKVFDISPIETVWGIGYKWV
CCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]