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The map label for this gene is spaK [H]

Identifier: 153934620

GI number: 153934620

Start: 1686540

End: 1687919

Strand: Direct

Name: spaK [H]

Synonym: CLC_1616

Alternate gene names: 153934620

Gene position: 1686540-1687919 (Clockwise)

Preceding gene: 153937086

Following gene: 153935569

Centisome position: 44.85

GC content: 27.75

Gene sequence:

>1380_bases
ATGGGTATAAATAAAAAACAGGTCACATTAAAAACTGTTTTTTTTAGATATCTATTAGCATTAGCTCTTGCTTTTTTTGT
AGCAATAATAATTGAAATAATTATAGCAAGCTTAGGGATGCAAATGAATCTTTTTAATAGTGCTAATTATAGTGAAGATT
TAGCAAGACGAGCAAAGCCTATACTTCAATCTTCGGAAAAAATTACAGAAGACATGATTCCTAAGGGGTGTAAATTTGCT
GTATTTGATAAAGAATTTAAGGTCATTAAGACAGATCTTAAGGGGGAAGATTTAAAACAAGCAACTTTATATGCTAAAGG
CATTGATAGAAAAAATGGGTATAAGAAAAGTTATTATTTTATTGAAAGAAAAGATGGCTTTTGTGTATTGCAATATTATA
TCAAAATGAGCTATTCTTCAGAATGGATGAATGAACATTTTCCTAATCCAGAATTTTTATTAATTACAACTCTTATTTTT
GGATGTTTAATTGGAGCTTTTATTATTTCCATAGCTTTTGCAAAAAATTTAAAAAATAATTTAATTCCTTTAATGGAGGC
TACTGAAAAAATAAAAGAGCAGGATTTAGATTTTGACATTGGAAATTCTCCTATAAAGGAATTTAATGATGTGTTATGTT
CTATTTCTGATATGAAAGAGGAATTAAAGAAATCTCTAAAGGAGCAGTGGAACTTAGAGAAGACTAAAAAAGAGCAGATA
TCTTCTTTAGCTCATGATATCAAAACTCCTTTAACTATTATTAAAGGAAATACTGAACTTTTAAAAGACTCTGATCTTAA
TAATGAGCAGAAAGAATATATTGAGTTTATAGAAAAGAATTCAATACAGATTGAAAAGTATATAAAATTGCTTATTTATA
TGTCAAAAGGTAAATTAGAGTTTTTAGGAAAATTACAGAAAACTAATACAAAGGAATTTGCTTATGAGATTTATAATCAA
TTAATTGCTCTTGCGGGACCAAAGGAACTACAAATTAAATTCGAACAAAAAGATATACCAGAAAATATAATTATAAATAA
AGAAGCTATGCATAGAGCTATAATAAATGTAATCTCTAATGCAGTGGATTATTCACCTTTTAATAGTGAATTATATTTTT
ATGTTGAAGGTAAAAATGACTCTGTAGAGTTTTCGGTTATTGACAGTGGTAAAGGATTCTCTGGTGAGGATTTAAAAGCT
GCTAAGAAACAGTTCTATATGGGAGATTCAAGTAGAACATCAAAAACACATTGGGGTATGGGGCTCTATATTGCAGATTC
TATTGTAAAACAACATAATGGAGTTTTGACTCTTAAAAATTCACATAAAATAGGAGGGGCAAAGGTAATAATTAAAATAC
CTTTATTAAGTGACTTTTAG

Upstream 100 bases:

>100_bases
GTGATAGCTCTGCTATTACAGAGCATATTAAAAATATTAGAGCTAAGTTAAAAGTTTTTGATATATCACCAATAGAAACA
GTATGGGGGATTGGTTACAA

Downstream 100 bases:

>100_bases
GTCTGCTAAAGTTTATTTAGAATAATTATCTATAAGTAAAAATGACACAAAATAAATATTATAAAAATAAATCACTAAAA
ATTAGTTAGATTTCTGATTA

Product: putative antibiotic transporter sensor protein

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIIASLGMQMNLFNSANYSEDLARRAKPILQSSEKITEDMIPKGCKFA
VFDKEFKVIKTDLKGEDLKQATLYAKGIDRKNGYKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLITTLIF
GCLIGAFIISIAFAKNLKNNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLEKTKKEQI
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIEFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFAYEIYNQ
LIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPFNSELYFYVEGKNDSVEFSVIDSGKGFSGEDLKA
AKKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF

Sequences:

>Translated_459_residues
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIIASLGMQMNLFNSANYSEDLARRAKPILQSSEKITEDMIPKGCKFA
VFDKEFKVIKTDLKGEDLKQATLYAKGIDRKNGYKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLITTLIF
GCLIGAFIISIAFAKNLKNNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLEKTKKEQI
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIEFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFAYEIYNQ
LIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPFNSELYFYVEGKNDSVEFSVIDSGKGFSGEDLKA
AKKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
>Mature_458_residues
GINKKQVTLKTVFFRYLLALALAFFVAIIIEIIIASLGMQMNLFNSANYSEDLARRAKPILQSSEKITEDMIPKGCKFAV
FDKEFKVIKTDLKGEDLKQATLYAKGIDRKNGYKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLITTLIFG
CLIGAFIISIAFAKNLKNNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLEKTKKEQIS
SLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIEFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFAYEIYNQL
IALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPFNSELYFYVEGKNDSVEFSVIDSGKGFSGEDLKAA
KKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=253, Percent_Identity=26.8774703557312, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786783, Length=346, Percent_Identity=22.5433526011561, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=23.4513274336283, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=29.2237442922374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=21.9512195121951, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52541; Mature: 52410

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIIASLGMQMNLFNSANYSEDLARRAKP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
ILQSSEKITEDMIPKGCKFAVFDKEFKVIKTDLKGEDLKQATLYAKGIDRKNGYKKSYYF
HHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEE
IERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLITTLIFGCLIGAFIISIAFAKNLKNN
EECCCCEEEEEEHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLEKTKKEQI
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIEFIEKNSIQIEKYIKLLIYMSKGKLE
HHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
FLGKLQKTNTKEFAYEIYNQLIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISN
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
AVDYSPFNSELYFYVEGKNDSVEFSVIDSGKGFSGEDLKAAKKQFYMGDSSRTSKTHWGM
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
GLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
GINKKQVTLKTVFFRYLLALALAFFVAIIIEIIIASLGMQMNLFNSANYSEDLARRAKP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
ILQSSEKITEDMIPKGCKFAVFDKEFKVIKTDLKGEDLKQATLYAKGIDRKNGYKKSYYF
HHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEE
IERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLITTLIFGCLIGAFIISIAFAKNLKNN
EECCCCEEEEEEHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLEKTKKEQI
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIEFIEKNSIQIEKYIKLLIYMSKGKLE
HHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
FLGKLQKTNTKEFAYEIYNQLIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISN
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
AVDYSPFNSELYFYVEGKNDSVEFSVIDSGKGFSGEDLKAAKKQFYMGDSSRTSKTHWGM
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
GLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]