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The map label for this gene is gltA-1 [H]

Identifier: 153936605

GI number: 153936605

Start: 1760614

End: 1761996

Strand: Direct

Name: gltA-1 [H]

Synonym: CLC_1671

Alternate gene names: 153936605

Gene position: 1760614-1761996 (Clockwise)

Preceding gene: 153935790

Following gene: 153937365

Centisome position: 46.82

GC content: 34.78

Gene sequence:

>1383_bases
ATGGATAGAATGAAAAAGGTATCAGTTAGAGAACAAGATCCAAAGGTAAGAGCTACTAATTTTGAAGAAGTTTGTTTAGG
ATATAATGAAGAAGAAGCTGTTAAAGAAGCTAGTAGATGTTTAAATTGTAAAAAACCAATGTGTGTAACAAAATGTCCAG
TTTCAATAGGAATACCGGAATTTATACAACATGTAAAAGATAAAGAGTTTGAAGAGGCTGCAAAAACTATAGCTAAATAT
AGTGCATTACCAGCAGTCTGTGGAAGAGTCTGTCCTCAAGAAAGTCAATGTGAAGGAAAATGTGTACTTGGCATAAAAGG
GGAACCTGTAGCTATAGGTAAACTTGAAAGATTCGTTGCAGATTGGTCAAGGGAACATAATGTAGATTTATCCGAAAGAG
AAGAAAGTAAAAATAAAAAGGTAGCAGTAATAGGAAGTGGTCCAGCAGGACTTACTTGTGCAGGAGATTTAGCTAAAAAA
GGATACGATGTTACTATATTTGAAGCTCTTCATGAACCAGGTGGAGTTTTAGTATACGGAATACCAGAATTTAGATTACC
AAAGGAAACTGTGGTAAAACATGAAATAGAAAACGTTAAAAAATTAGGCGTTAAAATAGAAACTAATGTAATTATAGGAA
GAACAGTAACTATAGATGAACTTATAGAAGAAGAAAAATTTGATGCAGTATTTATAGGATCAGGAGCAGGACTTCCAAGA
TTTATGGGAATACCAGGAGAAAATGCAAATGGAGTGTTCTCAGCAAATGAATTTTTAACAAGAAATAATTTAATGAAAGC
TTTCAAGGATGAATATGATACACCTATAAAAGTAGGACAGAAGGTAGCTGTAGTAGGTGGCGGAAATGTTGCTATGGATG
CTGCTAGAACAGCCCTAAGACTTGGAGCAGAAGTTCATATAGTATATAGGAGATCAGAAGAAGAACTTCCAGCAAGGGTA
GAAGAAGTACACCATGCAAAGGAAGAAGGAATAATATTTAATCTTTTAACTAATCCAGTAGAAATATTAGAGGATGAAAA
AGGATGGGTTAAAGGAATGAAATGTATAAAGATGGAACTAGGAGAACCAGATGAGTCTGGCAGAAGGAGACCAGTAGAAA
TAAAAGATTCAGAATTTGTAATGGATGTAGATACAGTAATAATGTCTTTAGGAACTTCTCCAAACCCATTAATATCTTCT
ACTACAAAGGGATTAGAAATAAATAAGCGTAAATGCCTAGTAGCAGAAGAGGAAACAGGATTAACTACAAGAGAAGGGGT
ATATGCAGGTGGAGATGCAGTAACAGGCGCTGCTACTGTAATTTTAGCTATGGGAGCTGGAAAACAAGCTGCTACAGCCA
TAGATGAATATTTAAGTAAATAA

Upstream 100 bases:

>100_bases
GACAAGCTTTATATAAAACAGAAGAAGGAAAAAGGCTTCATAAAATAGAAGAAGAAAAAGAAGGTCACGTATGTCATATC
GGAGCGGGGGAGAATGCGTA

Downstream 100 bases:

>100_bases
ATCAATTATAAAAAACTTCAACAAAATGATGTTGAAGTTTTTTATTTTAATAATGATTTTAAAAATTTATTTTATAAATA
TTGTTAGATATAATATAGTA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDRMKKVSVREQDPKVRATNFEEVCLGYNEEEAVKEASRCLNCKKPMCVTKCPVSIGIPEFIQHVKDKEFEEAAKTIAKY
SALPAVCGRVCPQESQCEGKCVLGIKGEPVAIGKLERFVADWSREHNVDLSEREESKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKETVVKHEIENVKKLGVKIETNVIIGRTVTIDELIEEEKFDAVFIGSGAGLPR
FMGIPGENANGVFSANEFLTRNNLMKAFKDEYDTPIKVGQKVAVVGGGNVAMDAARTALRLGAEVHIVYRRSEEELPARV
EEVHHAKEEGIIFNLLTNPVEILEDEKGWVKGMKCIKMELGEPDESGRRRPVEIKDSEFVMDVDTVIMSLGTSPNPLISS
TTKGLEINKRKCLVAEEETGLTTREGVYAGGDAVTGAATVILAMGAGKQAATAIDEYLSK

Sequences:

>Translated_460_residues
MDRMKKVSVREQDPKVRATNFEEVCLGYNEEEAVKEASRCLNCKKPMCVTKCPVSIGIPEFIQHVKDKEFEEAAKTIAKY
SALPAVCGRVCPQESQCEGKCVLGIKGEPVAIGKLERFVADWSREHNVDLSEREESKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKETVVKHEIENVKKLGVKIETNVIIGRTVTIDELIEEEKFDAVFIGSGAGLPR
FMGIPGENANGVFSANEFLTRNNLMKAFKDEYDTPIKVGQKVAVVGGGNVAMDAARTALRLGAEVHIVYRRSEEELPARV
EEVHHAKEEGIIFNLLTNPVEILEDEKGWVKGMKCIKMELGEPDESGRRRPVEIKDSEFVMDVDTVIMSLGTSPNPLISS
TTKGLEINKRKCLVAEEETGLTTREGVYAGGDAVTGAATVILAMGAGKQAATAIDEYLSK
>Mature_460_residues
MDRMKKVSVREQDPKVRATNFEEVCLGYNEEEAVKEASRCLNCKKPMCVTKCPVSIGIPEFIQHVKDKEFEEAAKTIAKY
SALPAVCGRVCPQESQCEGKCVLGIKGEPVAIGKLERFVADWSREHNVDLSEREESKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKETVVKHEIENVKKLGVKIETNVIIGRTVTIDELIEEEKFDAVFIGSGAGLPR
FMGIPGENANGVFSANEFLTRNNLMKAFKDEYDTPIKVGQKVAVVGGGNVAMDAARTALRLGAEVHIVYRRSEEELPARV
EEVHHAKEEGIIFNLLTNPVEILEDEKGWVKGMKCIKMELGEPDESGRRRPVEIKDSEFVMDVDTVIMSLGTSPNPLISS
TTKGLEINKRKCLVAEEETGLTTREGVYAGGDAVTGAATVILAMGAGKQAATAIDEYLSK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=33.1905781584582, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI237757300, Length=106, Percent_Identity=36.7924528301887, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788811, Length=472, Percent_Identity=37.5, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789606, Length=464, Percent_Identity=36.2068965517241, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI87082180, Length=447, Percent_Identity=38.9261744966443, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=34.1628959276018, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789243, Length=419, Percent_Identity=33.4128878281623, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787114, Length=351, Percent_Identity=25.0712250712251, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984108, Length=450, Percent_Identity=30.8888888888889, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=488, Percent_Identity=28.2786885245902, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17543860, Length=177, Percent_Identity=35.0282485875706, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320030, Length=472, Percent_Identity=29.0254237288136, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320584, Length=176, Percent_Identity=32.9545454545455, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665547, Length=483, Percent_Identity=30.2277432712215, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24665543, Length=483, Percent_Identity=30.2277432712215, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI28574881, Length=483, Percent_Identity=30.2277432712215, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24665539, Length=483, Percent_Identity=30.2277432712215, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24640763, Length=444, Percent_Identity=31.3063063063063, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18858217, Length=444, Percent_Identity=31.3063063063063, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50213; Mature: 50213

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRMKKVSVREQDPKVRATNFEEVCLGYNEEEAVKEASRCLNCKKPMCVTKCPVSIGIPE
CCCCCCCCCCCCCCCEECCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
FIQHVKDKEFEEAAKTIAKYSALPAVCGRVCPQESQCEGKCVLGIKGEPVAIGKLERFVA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHH
DWSREHNVDLSEREESKNKKVAVIGSGPAGLTCAGDLAKKGYDVTIFEALHEPGGVLVYG
HHHHCCCCCCHHHHHCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEHHHCCCCCEEEEC
IPEFRLPKETVVKHEIENVKKLGVKIETNVIIGRTVTIDELIEEEKFDAVFIGSGAGLPR
CCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEEECEEEHHHHHHHCCCCEEEEECCCCCCC
FMGIPGENANGVFSANEFLTRNNLMKAFKDEYDTPIKVGQKVAVVGGGNVAMDAARTALR
EECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCHHHHHHHHHH
LGAEVHIVYRRSEEELPARVEEVHHAKEEGIIFNLLTNPVEILEDEKGWVKGMKCIKMEL
CCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCCHHHHHHHHEEEEC
GEPDESGRRRPVEIKDSEFVMDVDTVIMSLGTSPNPLISSTTKGLEINKRKCLVAEEETG
CCCCCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCC
LTTREGVYAGGDAVTGAATVILAMGAGKQAATAIDEYLSK
CCCCCCEEECCCCHHHHEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDRMKKVSVREQDPKVRATNFEEVCLGYNEEEAVKEASRCLNCKKPMCVTKCPVSIGIPE
CCCCCCCCCCCCCCCEECCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
FIQHVKDKEFEEAAKTIAKYSALPAVCGRVCPQESQCEGKCVLGIKGEPVAIGKLERFVA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHH
DWSREHNVDLSEREESKNKKVAVIGSGPAGLTCAGDLAKKGYDVTIFEALHEPGGVLVYG
HHHHCCCCCCHHHHHCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEHHHCCCCCEEEEC
IPEFRLPKETVVKHEIENVKKLGVKIETNVIIGRTVTIDELIEEEKFDAVFIGSGAGLPR
CCCCCCCHHHHHHHHHHHHHHHCEEEEEEEEEECEEEHHHHHHHCCCCEEEEECCCCCCC
FMGIPGENANGVFSANEFLTRNNLMKAFKDEYDTPIKVGQKVAVVGGGNVAMDAARTALR
EECCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCHHHHHHHHHH
LGAEVHIVYRRSEEELPARVEEVHHAKEEGIIFNLLTNPVEILEDEKGWVKGMKCIKMEL
CCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCCHHHHHHHHEEEEC
GEPDESGRRRPVEIKDSEFVMDVDTVIMSLGTSPNPLISSTTKGLEINKRKCLVAEEETG
CCCCCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCC
LTTREGVYAGGDAVTGAATVILAMGAGKQAATAIDEYLSK
CCCCCCEEECCCCHHHHEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]