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The map label for this gene is hisF

Identifier: 153936004

GI number: 153936004

Start: 1674340

End: 1675101

Strand: Direct

Name: hisF

Synonym: CLC_1603

Alternate gene names: 153936004

Gene position: 1674340-1675101 (Clockwise)

Preceding gene: 153936729

Following gene: 153936352

Centisome position: 44.52

GC content: 33.46

Gene sequence:

>762_bases
GTGATTACAAAGAGGATAATACCTTGCTTAGATGTAGATATGGGGAGAGTTGTAAAGGGAGTTAATTTTGTTAATCTAAA
GGATGTAGGTGATCCTGTAGAAATAGCAGAGTTTTATAACAAAGAAGGAGCAGATGAAATAGTATTTCTAGATATATCAG
CTACTCATGAAGGAAGAGCTACAATGATAGATGTGGTTAGAAAAACAGCAGAGAAACTATTTATCCCTCTTACTGTAGGA
GGGGGAATTAAAAATATAAATGATTTTAGAGACATATTAAGAGCTGGGGCAGATAAAATATCAGTTAATTCATCCGCTAT
AAGAAATCCTAAACTTATAAAAAAGGCAGCAGAATGTTTTGGATCACAATGTGTTGTAGTGGCTATAGATGGTAAAAAAC
GAAAAGATAAGGATGGATGGAATGTATTTATAAATGGAGGCAGAATAGATACAGGCCTTGATGCTATAGAGTGGGCAAGA
AAGGTTGAGAAATTAGGAGCCGGTGAGATATTATTAACAAGTATGGATGCAGATGGTACAAAAGAAGGTTATGATTTAGA
GTTTACAAATGAAGTTTCAAAGGCAGTAAATATTCCTGTAATTGCTTCAGGAGGATGCGGAAAACTGAAACATTTTGGAG
AAATTTTTGAAAAAAGTTCAGCAGATGCAGCATTAGCAGCTTCATTATTTCATTTTAAAGAATTGAGCATAAAGGAAGTT
AAAAACTATTTAAAAAAAGAAGGCTTTAGTGTAAGACTTTAG

Upstream 100 bases:

>100_bases
AAAAATCGGGTAAAAGTGTTATTGAAGTATAAAATTATGAAAAAAGAATATATGACTAAAAAATAGAAGTTTAAAAATAT
GCTAAAAAGGGGGAGTATAT

Downstream 100 bases:

>100_bases
CTTCATATATTTAAATTTTAAAATTTTATAGATAAATTTCATTCTATATAAAGATTATAAATGATTACAAATAGATGTTG
TAATTCTCATCTCCATATTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGLDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL

Sequences:

>Translated_253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGLDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL
>Mature_253_residues
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRATMIDVVRKTAEKLFIPLTVG
GGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECFGSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGLDAIEWAR
KVEKLGAGEILLTSMDADGTKEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
KNYLKKEGFSVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=45.3488372093023, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI87082028, Length=209, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.3503184713376, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CLOB1 (A7FU82)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383915.1
- ProteinModelPortal:   A7FU82
- SMR:   A7FU82
- STRING:   A7FU82
- GeneID:   5397917
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1592
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   CBOT441770:CLB_1592-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27610; Mature: 27610

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRA
CCCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
TMIDVVRKTAEKLFIPLTVGGGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHC
GSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGLDAIEWARKVEKLGAGEILLTSMDADGT
CCCEEEEEECCCCCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
CCCCCEEEHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHH
KNYLKKEGFSVRL
HHHHHHCCCEECC
>Mature Secondary Structure
MITKRIIPCLDVDMGRVVKGVNFVNLKDVGDPVEIAEFYNKEGADEIVFLDISATHEGRA
CCCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCH
TMIDVVRKTAEKLFIPLTVGGGIKNINDFRDILRAGADKISVNSSAIRNPKLIKKAAECF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHC
GSQCVVVAIDGKKRKDKDGWNVFINGGRIDTGLDAIEWARKVEKLGAGEILLTSMDADGT
CCCEEEEEECCCCCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KEGYDLEFTNEVSKAVNIPVIASGGCGKLKHFGEIFEKSSADAALAASLFHFKELSIKEV
CCCCCEEEHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHH
KNYLKKEGFSVRL
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA