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The map label for this gene is opuD [H]

Identifier: 153934602

GI number: 153934602

Start: 1218474

End: 1219988

Strand: Reverse

Name: opuD [H]

Synonym: CLC_1180

Alternate gene names: 153934602

Gene position: 1219988-1218474 (Counterclockwise)

Preceding gene: 153935613

Following gene: 153936932

Centisome position: 32.44

GC content: 29.44

Gene sequence:

>1515_bases
ATGATTAAAAAATTAAAAAAAGAAAAGGTTTTTTATGTATCAATTATAAGCTTATTATTTCTATTGTTATTGGCCTTCTT
TAATATTGATGCTTTTGCAAAATCCACTAAAAATATATTAAATTTCTTATTGGATAGGTTTTCTTGGTTTTATATACTGG
TTATGCTATCTTTTTTTATTTTTTGTATGTGGGCAGCTTTCAGTAAATATGGAAAACTAAAATTAGGAAAAGATAATGAA
GATCCCGAATATAGTTTAATTTCTTGGTTTACAATGCTTTTTAGTGCTGGTATGGGTATAGGCTTAATCTTCTGGGGAGT
TGCAGAACCTTTGAATCATTATATTCACCCATTTAATTTACAAGGATTTACAGAAGAAGCAAAAACCTTTGCTATGACAA
AATCATTTCTACATTGGGGTATTTCAGCCTGGTCTTGTTATGCTATTCTAGCTCTAGCATTAACTTATTTTCAGTTTAGA
AAAGGTAAACCAGCTTTAATGAGTAGTTTATTAATACCTCTAATTGGTGAAAAAAAAGCTTCCGGATGGATTGGAAATAC
AATAGATATTTTTACTATATTTGCAACTGTAGCGGGAGTAATTACTTCTCTTGGACTTGGTGCACTTCAAATTAATGCAG
GTTTAAATTACTTGCTTAATATTCCTGAGAACATTAAAGTTCAACTTATTATAATAATTTTAGTTACTATATGCTTTATA
GCTTCCGCATCTTCAGGTCTTAATAAAGGAATTCAAACTTTATCTAATTTAAATATGCTATTGGCAGCAGTTTTGTTAAT
ATTTGTAATATTAACAGGTCCTTCTTTAGAAATGAATAAAAATTTATTTAAAGGATTAGGTGTTTATGCAAAAGAACTTC
TAATAGATAATAATAACATATTTGTTACTGGTGATTGGTATAAAAAATGGACTATATTTTATTGGGGTTGGTGGATAGCT
TGGGCTCCTCCTGTGGGAATATTTATAGCTAGAATCTCTAAAGGGAGGACTATAAAAGAGTTTTTACTAGGAGTATTATT
TGTTCCATCAATAATATGTTTTATATGGTTTGCAGTATTTGGAACTATGGGCTTTAATGTAAGTCATACTGTTGCACTTA
CTGCAATTACAAGAGCTGAAACAGCTTTCTTTGTTGTTATGAATGAATATCATTTTGGATATATCATCTCCTTAATAGCT
ATAATGCTTCTAGGAACTTTCTTTGTAACTTCAGCGGACTCTGCAACTTTTGTATTGGGTATGCTTTCCTCTAATGGAGA
CTTAAATCCTAAAACTTCTAAAAAGTTTATTTGGGGAGTTCTTCAGGCTTCTTTAACTGTTGTTTTTCTAATAGCCGGAG
GCTTAGATATGATACAGACAGTTTCTATAATAGCAGCCTTTCCATTTGCTTTTATAATGATAATTGCAATGATTTCTCTA
AGGAAAAGTTTAAAAAATGAATCTCTTGAAATATCTTCACTTTCCAATAATAAAAAAATTGAAAAAGTTATATAA

Upstream 100 bases:

>100_bases
TTTTAGTAAATTTTACAATTTTATGCTTATATGCTATAATTAGAATCACAATCTGACACTCTTATATATTTTGTAAGTTG
TCAGTTATGGGAGGGTTTGT

Downstream 100 bases:

>100_bases
TTAATATATTAAGGGAGGATTCTCTTATTTTAGTGTCCTCCCTTAATATTTAATTTACTTATTTCAAATTTTATTAGCTA
AATCCCTTATTTTTTATTTC

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MIKKLKKEKVFYVSIISLLFLLLLAFFNIDAFAKSTKNILNFLLDRFSWFYILVMLSFFIFCMWAAFSKYGKLKLGKDNE
DPEYSLISWFTMLFSAGMGIGLIFWGVAEPLNHYIHPFNLQGFTEEAKTFAMTKSFLHWGISAWSCYAILALALTYFQFR
KGKPALMSSLLIPLIGEKKASGWIGNTIDIFTIFATVAGVITSLGLGALQINAGLNYLLNIPENIKVQLIIIILVTICFI
ASASSGLNKGIQTLSNLNMLLAAVLLIFVILTGPSLEMNKNLFKGLGVYAKELLIDNNNIFVTGDWYKKWTIFYWGWWIA
WAPPVGIFIARISKGRTIKEFLLGVLFVPSIICFIWFAVFGTMGFNVSHTVALTAITRAETAFFVVMNEYHFGYIISLIA
IMLLGTFFVTSADSATFVLGMLSSNGDLNPKTSKKFIWGVLQASLTVVFLIAGGLDMIQTVSIIAAFPFAFIMIIAMISL
RKSLKNESLEISSLSNNKKIEKVI

Sequences:

>Translated_504_residues
MIKKLKKEKVFYVSIISLLFLLLLAFFNIDAFAKSTKNILNFLLDRFSWFYILVMLSFFIFCMWAAFSKYGKLKLGKDNE
DPEYSLISWFTMLFSAGMGIGLIFWGVAEPLNHYIHPFNLQGFTEEAKTFAMTKSFLHWGISAWSCYAILALALTYFQFR
KGKPALMSSLLIPLIGEKKASGWIGNTIDIFTIFATVAGVITSLGLGALQINAGLNYLLNIPENIKVQLIIIILVTICFI
ASASSGLNKGIQTLSNLNMLLAAVLLIFVILTGPSLEMNKNLFKGLGVYAKELLIDNNNIFVTGDWYKKWTIFYWGWWIA
WAPPVGIFIARISKGRTIKEFLLGVLFVPSIICFIWFAVFGTMGFNVSHTVALTAITRAETAFFVVMNEYHFGYIISLIA
IMLLGTFFVTSADSATFVLGMLSSNGDLNPKTSKKFIWGVLQASLTVVFLIAGGLDMIQTVSIIAAFPFAFIMIIAMISL
RKSLKNESLEISSLSNNKKIEKVI
>Mature_504_residues
MIKKLKKEKVFYVSIISLLFLLLLAFFNIDAFAKSTKNILNFLLDRFSWFYILVMLSFFIFCMWAAFSKYGKLKLGKDNE
DPEYSLISWFTMLFSAGMGIGLIFWGVAEPLNHYIHPFNLQGFTEEAKTFAMTKSFLHWGISAWSCYAILALALTYFQFR
KGKPALMSSLLIPLIGEKKASGWIGNTIDIFTIFATVAGVITSLGLGALQINAGLNYLLNIPENIKVQLIIIILVTICFI
ASASSGLNKGIQTLSNLNMLLAAVLLIFVILTGPSLEMNKNLFKGLGVYAKELLIDNNNIFVTGDWYKKWTIFYWGWWIA
WAPPVGIFIARISKGRTIKEFLLGVLFVPSIICFIWFAVFGTMGFNVSHTVALTAITRAETAFFVVMNEYHFGYIISLIA
IMLLGTFFVTSADSATFVLGMLSSNGDLNPKTSKKFIWGVLQASLTVVFLIAGGLDMIQTVSIIAAFPFAFIMIIAMISL
RKSLKNESLEISSLSNNKKIEKVI

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=507, Percent_Identity=35.5029585798817, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1788102, Length=444, Percent_Identity=28.6036036036036, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786224, Length=503, Percent_Identity=29.2246520874751, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56326; Mature: 56326

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLKKEKVFYVSIISLLFLLLLAFFNIDAFAKSTKNILNFLLDRFSWFYILVMLSFFI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCMWAAFSKYGKLKLGKDNEDPEYSLISWFTMLFSAGMGIGLIFWGVAEPLNHYIHPFNL
HHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
QGFTEEAKTFAMTKSFLHWGISAWSCYAILALALTYFQFRKGKPALMSSLLIPLIGEKKA
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
SGWIGNTIDIFTIFATVAGVITSLGLGALQINAGLNYLLNIPENIKVQLIIIILVTICFI
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHH
ASASSGLNKGIQTLSNLNMLLAAVLLIFVILTGPSLEMNKNLFKGLGVYAKELLIDNNNI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCE
FVTGDWYKKWTIFYWGWWIAWAPPVGIFIARISKGRTIKEFLLGVLFVPSIICFIWFAVF
EEEECCCEEEEEEEEEHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GTMGFNVSHTVALTAITRAETAFFVVMNEYHFGYIISLIAIMLLGTFFVTSADSATFVLG
HHCCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
MLSSNGDLNPKTSKKFIWGVLQASLTVVFLIAGGLDMIQTVSIIAAFPFAFIMIIAMISL
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSLKNESLEISSLSNNKKIEKVI
HHHHCCCCEEEEECCCCCCHHHCC
>Mature Secondary Structure
MIKKLKKEKVFYVSIISLLFLLLLAFFNIDAFAKSTKNILNFLLDRFSWFYILVMLSFFI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCMWAAFSKYGKLKLGKDNEDPEYSLISWFTMLFSAGMGIGLIFWGVAEPLNHYIHPFNL
HHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
QGFTEEAKTFAMTKSFLHWGISAWSCYAILALALTYFQFRKGKPALMSSLLIPLIGEKKA
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
SGWIGNTIDIFTIFATVAGVITSLGLGALQINAGLNYLLNIPENIKVQLIIIILVTICFI
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHH
ASASSGLNKGIQTLSNLNMLLAAVLLIFVILTGPSLEMNKNLFKGLGVYAKELLIDNNNI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCE
FVTGDWYKKWTIFYWGWWIAWAPPVGIFIARISKGRTIKEFLLGVLFVPSIICFIWFAVF
EEEECCCEEEEEEEEEHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GTMGFNVSHTVALTAITRAETAFFVVMNEYHFGYIISLIAIMLLGTFFVTSADSATFVLG
HHCCCCCHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
MLSSNGDLNPKTSKKFIWGVLQASLTVVFLIAGGLDMIQTVSIIAAFPFAFIMIIAMISL
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSLKNESLEISSLSNNKKIEKVI
HHHHCCCCEEEEECCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]