Click here to switch to the map view.

The map label for this gene is sigG [H]

Identifier: 153933176

GI number: 153933176

Start: 2542472

End: 2543245

Strand: Reverse

Name: sigG [H]

Synonym: CLB_2408

Alternate gene names: 153933176

Gene position: 2543245-2542472 (Counterclockwise)

Preceding gene: 153932419

Following gene: 153933553

Centisome position: 65.83

GC content: 29.59

Gene sequence:

>774_bases
ATGGTTATTAATAAAGTTGAAATTTGTGGAGTAAATACATCAAAATTACCAGTATTAAAGGATAAAGAGATGAAAAAATT
ACTGGTAAGGATTAGGAACGGTGAAACGGAATGTAGAGAAGAATTTATACAAGGAAATTTAAGGTTAGTATTAAGTGTAA
TAAAAAGATTTAACAATCGAGGGGAAAATGTGGATGATCTTTTTCAAGTTGGATGTATAGGCCTTATAAAAGCCATAGAC
AATTTTGATTTAAGTCAAAATGTAAAATTCTCTACTTATGCTGTTCCTATGATAATAGGAGAAATAAGAAGATACCTAAG
AGATAATAATTCCATAAGAGTTAGTAGATCTTTAAGAGATATAGCCTATAAAGCTCTACAGGCTAGAGACAAATTAATAA
AAAATAATAATAAAGAACCAACAGTGTCTCAAATAGCTAAAGAATTAGAACTACCAAGAGAAGAGGTAGTGTTTGCTTTA
GACGCTATACAGGATCCAGTGTCTTTGTTTGAACCTATATATCATGATGGTGGTGACGCCATATTTGTTATGGATCAAAT
AAGTGACACTAAAAATATAGATGAGAATTGGATAGAAAATATATCTATAAAAGAAGCTATGAAAAAATTAAATGACAGAG
AAAAACTAATACTTAATTTAAGGTTTTTTGATGGAAGAACTCAAATGGAAGTAGCCGATGAAATAGGCATATCTCAAGCA
CAGGTATCTAGGTTAGAAAAAACTGCTTTAAAACATATGAGAAAGTATGTTTAA

Upstream 100 bases:

>100_bases
AAAAAGAAATAAATAAAATGGTTTAGTTTGTCCTTAGTATAAAATTAGATTCCTTTGGAAACAATTTAAATGCAATCATT
CTGAAGGGACTGATGACTTT

Downstream 100 bases:

>100_bases
TCAAAAAATGTTCTTAAATTATTAGAGAACACTTTAAGCTACTGATAAATATTTTTTATCAGTAGCTTTTTATTTTTTAA
TGTTTTTCATAGAATAGGAT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MVINKVEICGVNTSKLPVLKDKEMKKLLVRIRNGETECREEFIQGNLRLVLSVIKRFNNRGENVDDLFQVGCIGLIKAID
NFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQARDKLIKNNNKEPTVSQIAKELELPREEVVFAL
DAIQDPVSLFEPIYHDGGDAIFVMDQISDTKNIDENWIENISIKEAMKKLNDREKLILNLRFFDGRTQMEVADEIGISQA
QVSRLEKTALKHMRKYV

Sequences:

>Translated_257_residues
MVINKVEICGVNTSKLPVLKDKEMKKLLVRIRNGETECREEFIQGNLRLVLSVIKRFNNRGENVDDLFQVGCIGLIKAID
NFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQARDKLIKNNNKEPTVSQIAKELELPREEVVFAL
DAIQDPVSLFEPIYHDGGDAIFVMDQISDTKNIDENWIENISIKEAMKKLNDREKLILNLRFFDGRTQMEVADEIGISQA
QVSRLEKTALKHMRKYV
>Mature_257_residues
MVINKVEICGVNTSKLPVLKDKEMKKLLVRIRNGETECREEFIQGNLRLVLSVIKRFNNRGENVDDLFQVGCIGLIKAID
NFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQARDKLIKNNNKEPTVSQIAKELELPREEVVFAL
DAIQDPVSLFEPIYHDGGDAIFVMDQISDTKNIDENWIENISIKEAMKKLNDREKLILNLRFFDGRTQMEVADEIGISQA
QVSRLEKTALKHMRKYV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789098, Length=249, Percent_Identity=27.710843373494, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789871, Length=259, Percent_Identity=27.027027027027, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788231, Length=223, Percent_Identity=27.3542600896861, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29629; Mature: 29629

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVINKVEICGVNTSKLPVLKDKEMKKLLVRIRNGETECREEFIQGNLRLVLSVIKRFNNR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
GENVDDLFQVGCIGLIKAIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
IAYKALQARDKLIKNNNKEPTVSQIAKELELPREEVVFALDAIQDPVSLFEPIYHDGGDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCE
IFVMDQISDTKNIDENWIENISIKEAMKKLNDREKLILNLRFFDGRTQMEVADEIGISQA
EEEEECCCCCCCCCHHHHHCCCHHHHHHHHCCCHHEEEEEEEECCCCHHHHHHHHCCCHH
QVSRLEKTALKHMRKYV
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVINKVEICGVNTSKLPVLKDKEMKKLLVRIRNGETECREEFIQGNLRLVLSVIKRFNNR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
GENVDDLFQVGCIGLIKAIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
IAYKALQARDKLIKNNNKEPTVSQIAKELELPREEVVFALDAIQDPVSLFEPIYHDGGDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCE
IFVMDQISDTKNIDENWIENISIKEAMKKLNDREKLILNLRFFDGRTQMEVADEIGISQA
EEEEECCCCCCCCCHHHHHCCCHHHHHHHHCCCHHEEEEEEEECCCCHHHHHHHHCCCHH
QVSRLEKTALKHMRKYV
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961408; 7883192; 11466286 [H]