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The map label for this gene is sigE [H]

Identifier: 153932419

GI number: 153932419

Start: 2543320

End: 2544027

Strand: Reverse

Name: sigE [H]

Synonym: CLB_2409

Alternate gene names: 153932419

Gene position: 2544027-2543320 (Counterclockwise)

Preceding gene: 153931338

Following gene: 153933176

Centisome position: 65.85

GC content: 26.84

Gene sequence:

>708_bases
ATGATAAATTTAAAGGTATTATTAAACAGAATATTAATAAAATTTAAATTGTTTTTTAAAAGCGTTTATTACATAGGTGG
GAATGATGCACTTCCACCGCCACTTTCAAAAGAAGAAGAAGAATATTTTGTACAAAGGTTAATAAATGGAGATGAAAAAG
TTCGATCCGTTTTAATAGAAAGAAATTTAAGATTAGTGGTTTATATAGCTAGAAAATTTGAAAATACAGGCATATGTATA
GAAGATCTAGTATCCGTAGGAACTATAGGACTAATTAAGGCAGTAAATACTTTTAAACCAGACAAAAAAATTAAATTAGC
AACCTATGCTTCAAGATGTATAGAAAATGAGATATTAATGTATTTAAGAAGAAATAGTAAAGTAAAAGCAGAAATATCCT
TTTATGAACCTTTAAATATTGATTGGGATGGAAATGAGCTATTACTTTCAGATATATTGGGAACAGACAATGATGAGGTC
TATAATTTAATAGAAGATGAAGTAGATAAACAATTACTATTATTAGCTATGAAAAAATTGAACGAAAGAGAAAAGGAAAT
AGTAAGATTAAGATTTGGTCTTAATGGGAAGAGAGAAAAAACTCAAAAAGAAGTAGCAGATATGCTGGGTATATCTCAGT
CCTATATATCAAGGCTAGAAAAAAGAATAATTAAGACTCTAAAAAAAGAAATAAATAAAATGGTTTAG

Upstream 100 bases:

>100_bases
AAAAAAAAGAAGTTATAATAGCACTTTCAGAAGGTAAACTTAGTGGTATAAAAGACTATAGAGCACTTTTATCCCGAGGA
ATTATATAGGAGGGACTTTT

Downstream 100 bases:

>100_bases
TTTGTCCTTAGTATAAAATTAGATTCCTTTGGAAACAATTTAAATGCAATCATTCTGAAGGGACTGATGACTTTATGGTT
ATTAATAAAGTTGAAATTTG

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MINLKVLLNRILIKFKLFFKSVYYIGGNDALPPPLSKEEEEYFVQRLINGDEKVRSVLIERNLRLVVYIARKFENTGICI
EDLVSVGTIGLIKAVNTFKPDKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDNDEV
YNLIEDEVDKQLLLLAMKKLNEREKEIVRLRFGLNGKREKTQKEVADMLGISQSYISRLEKRIIKTLKKEINKMV

Sequences:

>Translated_235_residues
MINLKVLLNRILIKFKLFFKSVYYIGGNDALPPPLSKEEEEYFVQRLINGDEKVRSVLIERNLRLVVYIARKFENTGICI
EDLVSVGTIGLIKAVNTFKPDKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDNDEV
YNLIEDEVDKQLLLLAMKKLNEREKEIVRLRFGLNGKREKTQKEVADMLGISQSYISRLEKRIIKTLKKEINKMV
>Mature_235_residues
MINLKVLLNRILIKFKLFFKSVYYIGGNDALPPPLSKEEEEYFVQRLINGDEKVRSVLIERNLRLVVYIARKFENTGICI
EDLVSVGTIGLIKAVNTFKPDKKIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDNDEV
YNLIEDEVDKQLLLLAMKKLNEREKEIVRLRFGLNGKREKTQKEVADMLGISQSYISRLEKRIIKTLKKEINKMV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=28.5198555956679, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=37.7358490566038, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27348; Mature: 27348

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLKVLLNRILIKFKLFFKSVYYIGGNDALPPPLSKEEEEYFVQRLINGDEKVRSVLIE
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
RNLRLVVYIARKFENTGICIEDLVSVGTIGLIKAVNTFKPDKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
YLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDNDEVYNLIEDEVDKQLLLLAMKKL
HHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NEREKEIVRLRFGLNGKREKTQKEVADMLGISQSYISRLEKRIIKTLKKEINKMV
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINLKVLLNRILIKFKLFFKSVYYIGGNDALPPPLSKEEEEYFVQRLINGDEKVRSVLIE
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
RNLRLVVYIARKFENTGICIEDLVSVGTIGLIKAVNTFKPDKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
YLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDNDEVYNLIEDEVDKQLLLLAMKKL
HHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NEREKEIVRLRFGLNGKREKTQKEVADMLGISQSYISRLEKRIIKTLKKEINKMV
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883192; 11466286; 7961408 [H]