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The map label for this gene is rlmN

Identifier: 153932990

GI number: 153932990

Start: 2513726

End: 2514754

Strand: Reverse

Name: rlmN

Synonym: CLB_2379

Alternate gene names: 153932990

Gene position: 2514754-2513726 (Counterclockwise)

Preceding gene: 153931256

Following gene: 153931990

Centisome position: 65.09

GC content: 27.31

Gene sequence:

>1029_bases
ATGGAAAACATATTAGACTTTACATTAGAAGAACTAAAAGAATGGCTAATATCTAAAGAAGAAAAGGCTTTTAGAGCAAA
ACAAGTTTTTGATTGGATTTATAATAAACTTATTTTTGATTTTAATAATATGAAAAACATACCTTATAAAACTAAGAATT
TGCTTTCTGATAATTTTTATGTTGGTGTACCAAAAGTAGTTAAAAAATTAATGTCACAGGATAAAAACACCTATAAATTT
TTATTTGAGTATAAAGATGGAAATATAATAGAATCTGTGGTGATGAAATATAAACATGGTAATTCTATTTGTGTATCTAC
TCAAGTTGGATGTAGAATGGGCTGCAAATTTTGTGCATCTACTTTAGATGGCGTTATAAGAAATCTAACTAGTGGAGAAA
TATTATCTCAGATAATGGCAGCACAAAAAGAAATAGGTGAAAGAATTTCTAATGTGGTTTTAATGGGAAGTGGAGAACCT
TTAGATAATTTTGAAAATGTGACTAAATTTTTAGATTTAGTTACATCTGATACTACACTAAATATAGGACAAAGACATAT
AACCCTATCTACTTGTGGTATAGTACCCAAAATAAAAGAATTAGCAGACAAGAATTATAATATAACTTTGGCCATATCCC
TGCATTCACCAGAAGATTTATTAAGAAAAGAAATGATGCCCATAGCTAATAAATATTCTATAAAAGAGCTTATGGAAGCT
TGTGATTATTATATAAATAAAACTAACAGAAGAATAACTTTTGAATATGCATTGGTAAAAGGTAAAAATGATTCTATCAA
AGAAGCTAAAAAATTAAGTACAGTGTTAAAAGGAAAACTTTGTCATGTAAACTTAATCCCTGTAAATGAAATAAAAGAAA
ATAGCTATGAAAAATCCACATTAAAAAATATAGAATCCTTTGGTAATATTTTAAAAGAGAATGGGATAGAAACAACTATA
AGAAGAGAAATGGGGGCAGATATAAATGCTGCCTGTGGACAACTAAGAAGAAGTTATGTTTCAAAATAG

Upstream 100 bases:

>100_bases
ATATAATATATCATAAAGAAGGATTTGTATCCATATTGCCTAATGATAAAATGGATGGCTTTTTTATAGCTAAATTGAAG
AAATGTTAGTAGGTGTTAAG

Downstream 100 bases:

>100_bases
AGCAAAGGGGTGGAAAATATGTTGGGGATACTATCTGATATAGGTAATGTTAGAGAGATAAATGAAGACTTTGTAGATTA
TTATCAAGGAGACCGATTTA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYVGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK

Sequences:

>Translated_342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYVGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK
>Mature_342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYVGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=36.734693877551, Blast_Score=227, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CLOB1 (A7FW72)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384683.1
- ProteinModelPortal:   A7FW72
- STRING:   A7FW72
- GeneID:   5397078
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2379
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14454
- BioCyc:   CBOT441770:CLB_2379-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39029; Mature: 39029

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
VGVPKVVKKLMSQDKNTYKFLFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCAS
ECHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
TLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEPLDNFENVTKFLDLVTSDTTL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHCCCEE
NIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
ECCCEEEEEEHHCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHH
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKST
HHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
LKNIESFGNILKENGIETTIRREMGADINAACGQLRRSYVSK
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
VGVPKVVKKLMSQDKNTYKFLFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCAS
ECHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
TLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEPLDNFENVTKFLDLVTSDTTL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHCCCEE
NIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
ECCCEEEEEEHHCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHH
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKST
HHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
LKNIESFGNILKENGIETTIRREMGADINAACGQLRRSYVSK
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA