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The map label for this gene is eno [H]

Identifier: 152977353

GI number: 152977353

Start: 3737234

End: 3738526

Strand: Reverse

Name: eno [H]

Synonym: Bcer98_3678

Alternate gene names: 152977353

Gene position: 3738526-3737234 (Counterclockwise)

Preceding gene: 152977354

Following gene: 152977352

Centisome position: 91.47

GC content: 41.07

Gene sequence:

>1293_bases
ATGTCAACAATCATTGATGTTTATGCTCGCGAAGTCCTTGATTCTCGTGGTAACCCAACTGTAGAAGTAGAAGTTTACAC
AGAAGGCGGCGCATTCGGACGCGCATTAGTACCAAGTGGTGCATCTACTGGTGAATATGAAGCAGTAGAATTACGTGATG
GTGACAAATCTCGTTACCTAGGTAAAGGTGTGCTTAACGCAGTTAAAAACGTTAACGAAATTATCGCTCCAGAAATTGTT
GGTTTCGATGTAACTGACCAAGCTGGTATCGACCGTGCTATGATCGAATTAGATGGCACTCCAAACAAAGGTAAATTAGG
TGCAAACGCGATCCTTGGTGTTTCTATGGCAGTAGCTCACGCAGCAGCTGATTTCGTAGGTCTTCCATTATATCGTTACC
TTGGTGGATTCAATGCAAAACAATTACCAACTCCAATGATGAACATCATCAACGGTGGTTCTCACGCTGATAACAACGTG
GACTTCCAAGAATTCATGATTCTACCAGTTGGTGCTCCAACATTCAAAGAATCAATCCGTATGGGTGCTGAAGTATTCCA
TGCATTAAAAGCTGTATTACATGACAAAGGTCTTAACACTGCAGTAGGTGACGAAGGTGGATTCGCTCCAAACCTTGGTT
CTAACCGCGAAGCATTAGAAGTAATTATCGAAGCAATCGAAAAAGCGGGTTACAAAGCTGGTGAGGACGTATTCTTAGGA
ATGGACGTTGCTTCTTCTGAGTTCTACAACAAAGAAACTGGTAAATATGATCTTTCTGGTGAAGGTCGCAGCATGACTTC
TGCAGAAATGGTTGATTTCTACGAAGAACTTTGCAAAGACTTCCCAATCATCTCTATCGAAGATGGTTTAGACGAAAACG
ACTGGGATGGTCACAAATTATTAACTGAGCGTCTTGGCAAAAAAGTTCAATTAGTAGGTGACGACTTATTCGTAACAAAT
ACGAAGAAACTTGCTGAAGGTATCGAAAAAGGTATCTCTAACTCAATCTTAATTAAAGTGAACCAAATCGGTACTTTAAC
TGAAACTTTCGAAGCAATCGAAATGGCTAAACGTGCTGGTTACACAGCAGTTGTATCTCACCGTTCTGGTGAAACTGAAG
ATGCTACAATCGCTGACATCGCAGTTGCAACAAACGCTGGTCAAATCAAAACTGGTTCTATGAGCCGTACTGACCGTATT
GCTAAGTACAACCAATTATTACGCATCGAAGACGAACTAGGCGAAATCGCTGTTTACGATGGTTTAAAATCTTTCTACAA
CCTTAAAAAATAA

Upstream 100 bases:

>100_bases
CTCCAACAATGCTAACACTTCTAGGTGTTGAACAACCTAAAGAAATGACAGGTAAAACAATTATTAAATAATTTATGTAA
TTTAAAGGAGAGAAAATAGT

Downstream 100 bases:

>100_bases
TGGTAGAAAGTAAGAGCAGACCGTGAGAAATCACGGTCTGTTTTTTTATGTAAATGAAATGTGAGATAATGTTTTATCGC
AATAGCGAATGCTAAAATTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSTIIDVYAREVLDSRGNPTVEVEVYTEGGAFGRALVPSGASTGEYEAVELRDGDKSRYLGKGVLNAVKNVNEIIAPEIV
GFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNV
DFQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGEDVFLG
MDVASSEFYNKETGKYDLSGEGRSMTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERLGKKVQLVGDDLFVTN
TKKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRI
AKYNQLLRIEDELGEIAVYDGLKSFYNLKK

Sequences:

>Translated_430_residues
MSTIIDVYAREVLDSRGNPTVEVEVYTEGGAFGRALVPSGASTGEYEAVELRDGDKSRYLGKGVLNAVKNVNEIIAPEIV
GFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNV
DFQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGEDVFLG
MDVASSEFYNKETGKYDLSGEGRSMTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERLGKKVQLVGDDLFVTN
TKKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRI
AKYNQLLRIEDELGEIAVYDGLKSFYNLKK
>Mature_429_residues
STIIDVYAREVLDSRGNPTVEVEVYTEGGAFGRALVPSGASTGEYEAVELRDGDKSRYLGKGVLNAVKNVNEIIAPEIVG
FDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVD
FQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGEDVFLGM
DVASSEFYNKETGKYDLSGEGRSMTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERLGKKVQLVGDDLFVTNT
KKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRIA
KYNQLLRIEDELGEIAVYDGLKSFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=54.0603248259861, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.8604118993135, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=53.3333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=53.3333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=48.729792147806, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=67.2897196261682, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.292343387471, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.292343387471, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=47.1204188481675, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=430, Percent_Identity=51.3953488372093, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=49.7706422018349, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=49.7706422018349, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=49.7706422018349, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=51.1627906976744, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=53.2110091743119, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=53.2110091743119, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=53.2110091743119, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=53.2110091743119, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=53.2110091743119, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=53.2110091743119, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46402; Mature: 46271

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIDVYAREVLDSRGNPTVEVEVYTEGGAFGRALVPSGASTGEYEAVELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLNAVKNVNEIIAPEIVGFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAH
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGEDVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVASSEFYNKETGKYDLSGEGRSMTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKL
CCHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGKKVQLVGDDLFVTNTKKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRIAKYNQLLRIEDELGEIAVYD
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHEEHHHHCCCHHHH
GLKSFYNLKK
HHHHHHCCCC
>Mature Secondary Structure 
STIIDVYAREVLDSRGNPTVEVEVYTEGGAFGRALVPSGASTGEYEAVELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHHH
GKGVLNAVKNVNEIIAPEIVGFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAH
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGEDVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVASSEFYNKETGKYDLSGEGRSMTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKL
CCHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERLGKKVQLVGDDLFVTNTKKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRIAKYNQLLRIEDELGEIAVYD
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHEEHHHHCCCHHHH
GLKSFYNLKK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA