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The map label for this gene is gcvH [H]

Identifier: 152977265

GI number: 152977265

Start: 3644847

End: 3645230

Strand: Reverse

Name: gcvH [H]

Synonym: Bcer98_3586

Alternate gene names: 152977265

Gene position: 3645230-3644847 (Counterclockwise)

Preceding gene: 152977266

Following gene: 152977264

Centisome position: 89.19

GC content: 38.54

Gene sequence:

>384_bases
ATGAGCATTCCAAATCATTTACGTTACTCTGAAGAACACGAATGGGTAAAAACTGAAGGGAATCAAGTTGTTATCGGTAT
TACTCATTTCGCACAAAGTGAGCTAGGCGATATCGTATTCGTTGAACTTCCTGAAGTAGGTGCAACACTTGAAGCTAATG
AGCCATTCGGAAGCGTAGAATCTGTTAAAACAGTTTCTGAATTATACGCACCTGTAAGTGGTAAAGTTGTAGCAGTAAAC
GAAGAATTAAGCGATCAACCAGAACTTGTTAACGAGTCTCCATACGAAGGTGCATGGATGGTGAAAGTTGAACTTTCTGA
TGCAAGTGAAGTTGAAAAACTATTAACTGCAGAAAAATACGCAGAAATGACAAACCAAGACTAA

Upstream 100 bases:

>100_bases
ATGTAACACTTGGCTTTAATGAAGAGCAGTTTGAAAGTATGTGGAAAAAGTACCAATAAGGTATTTTTACATAATTAATA
CATTCACTGGAGGTACAGTC

Downstream 100 bases:

>100_bases
TTTGATTCTAGATAAAAGAGGCAGCCTATGTGCTGTCTTTTTTAATTTTTATAGTACATTGTTATCAAAAGGAATGAGAA
ACCATATCCCCCTTACATTC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSIPNHLRYSEEHEWVKTEGNQVVIGITHFAQSELGDIVFVELPEVGATLEANEPFGSVESVKTVSELYAPVSGKVVAVN
EELSDQPELVNESPYEGAWMVKVELSDASEVEKLLTAEKYAEMTNQD

Sequences:

>Translated_127_residues
MSIPNHLRYSEEHEWVKTEGNQVVIGITHFAQSELGDIVFVELPEVGATLEANEPFGSVESVKTVSELYAPVSGKVVAVN
EELSDQPELVNESPYEGAWMVKVELSDASEVEKLLTAEKYAEMTNQD
>Mature_126_residues
SIPNHLRYSEEHEWVKTEGNQVVIGITHFAQSELGDIVFVELPEVGATLEANEPFGSVESVKTVSELYAPVSGKVVAVNE
ELSDQPELVNESPYEGAWMVKVELSDASEVEKLLTAEKYAEMTNQD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=48.6725663716814, Blast_Score=123, Evalue=4e-29,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=47.787610619469, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=54.1666666666667, Blast_Score=135, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=40.650406504065, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=44.3478260869565, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=40.495867768595, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=47.3684210526316, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14040; Mature: 13908

Theoretical pI: Translated: 3.91; Mature: 3.91

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNHLRYSEEHEWVKTEGNQVVIGITHFAQSELGDIVFVELPEVGATLEANEPFGSVE
CCCCCCCCCCCCCCEEECCCCEEEEEEHHHCHHCCCCEEEEEECCCCCEEECCCCCCCHH
SVKTVSELYAPVSGKVVAVNEELSDQPELVNESPYEGAWMVKVELSDASEVEKLLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
AEMTNQD
HHHCCCC
>Mature Secondary Structure 
SIPNHLRYSEEHEWVKTEGNQVVIGITHFAQSELGDIVFVELPEVGATLEANEPFGSVE
CCCCCCCCCCCCCEEECCCCEEEEEEHHHCHHCCCCEEEEEECCCCCEEECCCCCCCHH
SVKTVSELYAPVSGKVVAVNEELSDQPELVNESPYEGAWMVKVELSDASEVEKLLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
AEMTNQD
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA