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The map label for this gene is rluF [H]

Identifier: 152976935

GI number: 152976935

Start: 3303929

End: 3304618

Strand: Reverse

Name: rluF [H]

Synonym: Bcer98_3238

Alternate gene names: 152976935

Gene position: 3304618-3303929 (Counterclockwise)

Preceding gene: 152976936

Following gene: 152976934

Centisome position: 80.86

GC content: 37.39

Gene sequence:

>690_bases
ATGAAAATTAATCAATTTATAAGTGAAGCCCTCTCTTGTTCAAGGCGAGAAACAGATCGTCTTATTAAAGCTGGGCGCGT
TGCAATGAATGATAAAGTTTGTACGCATGGTGTACTTGTAAAACCCGATGATGTTGTCACGGTTGATGGGAAAGTTATTG
AAAAAGAAGAAAAAGAAAAAGTATATATAGCTTTCCATAAACCAGTTGGCATCACTTGCACAGCTGCGAAACATATCGAA
GGGAATATTATTGCTTATATCAACTATCCCGAAAGAATCTTTCCAGTTGGCCGTTTAGATAAAGCATCAGAAGGATTAAT
TTTACTAACAAACGATGGGAAGATTGCCAATCAAATTTTACATGGAGATCATGGACATGAGAAAGAATATGTCGTAACTG
TTGATAAGTCGTTTACGGATGGGTTTATTCATGGCATGAGTTGCGGTGTGAAAATTAAAGATGGAATGACAAAGCCTTGT
AAAGTAACGCGAGTAGATGACTCTACATTTCGCATCGTTTTAACACAAGGAATGAATCGACAAATTCGGAGAATGAGCCG
TGCTTTTGGTTATACAGTAACAAAACTAAAGCGCGTTCGAATTATGAATATAAAATTAGGTGAGCTTGAGGTTGGAAAGT
GGCGCTATCTTACCGATGAAGAGTTACAAGAGTTACGAGGAGCATTATAG

Upstream 100 bases:

>100_bases
GGTTTTTGCAAAGTAAAGAAGTGTCAATCTCGGCATCATACTATGCTTTGAGATTGATGCTTTTTCTTTTTGGACAATCT
ATAAGAAAAAGGAATAGACA

Downstream 100 bases:

>100_bases
GGAGAGTTGTTTGCTCCGTATGTTTTTTGTCAGAAACAATAAAACTGTTGCTTTTTTTTAAAATACCCCATATAATAAAA
AACAACAAGTTTCGACAATA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKINQFISEALSCSRRETDRLIKAGRVAMNDKVCTHGVLVKPDDVVTVDGKVIEKEEKEKVYIAFHKPVGITCTAAKHIE
GNIIAYINYPERIFPVGRLDKASEGLILLTNDGKIANQILHGDHGHEKEYVVTVDKSFTDGFIHGMSCGVKIKDGMTKPC
KVTRVDDSTFRIVLTQGMNRQIRRMSRAFGYTVTKLKRVRIMNIKLGELEVGKWRYLTDEELQELRGAL

Sequences:

>Translated_229_residues
MKINQFISEALSCSRRETDRLIKAGRVAMNDKVCTHGVLVKPDDVVTVDGKVIEKEEKEKVYIAFHKPVGITCTAAKHIE
GNIIAYINYPERIFPVGRLDKASEGLILLTNDGKIANQILHGDHGHEKEYVVTVDKSFTDGFIHGMSCGVKIKDGMTKPC
KVTRVDDSTFRIVLTQGMNRQIRRMSRAFGYTVTKLKRVRIMNIKLGELEVGKWRYLTDEELQELRGAL
>Mature_229_residues
MKINQFISEALSCSRRETDRLIKAGRVAMNDKVCTHGVLVKPDDVVTVDGKVIEKEEKEKVYIAFHKPVGITCTAAKHIE
GNIIAYINYPERIFPVGRLDKASEGLILLTNDGKIANQILHGDHGHEKEYVVTVDKSFTDGFIHGMSCGVKIKDGMTKPC
KVTRVDDSTFRIVLTQGMNRQIRRMSRAFGYTVTKLKRVRIMNIKLGELEVGKWRYLTDEELQELRGAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=226, Percent_Identity=52.6548672566372, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=33.4728033472803, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788510, Length=228, Percent_Identity=28.0701754385965, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=30.8219178082192, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 25878; Mature: 25878

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINQFISEALSCSRRETDRLIKAGRVAMNDKVCTHGVLVKPDDVVTVDGKVIEKEEKEK
CCHHHHHHHHHHCCHHHHHHHHHHCCEEECCCEECCCEEECCCCEEEECCEEECCCCCCE
VYIAFHKPVGITCTAAKHIEGNIIAYINYPERIFPVGRLDKASEGLILLTNDGKIANQIL
EEEEEECCCCEEEEEEEECCCCEEEEEECCHHCCCCCCCCCCCCCEEEEECCCHHHHHHH
HGDHGHEKEYVVTVDKSFTDGFIHGMSCGVKIKDGMTKPCKVTRVDDSTFRIVLTQGMNR
CCCCCCCCCEEEEEECCCCCCEEEEEECCEEECCCCCCCEEEEEECCCEEEEEEECCCCH
QIRRMSRAFGYTVTKLKRVRIMNIKLGELEVGKWRYLTDEELQELRGAL
HHHHHHHHHCHHHHHEEEEEEEEEEECCEECCCEEECCHHHHHHHHCCC
>Mature Secondary Structure
MKINQFISEALSCSRRETDRLIKAGRVAMNDKVCTHGVLVKPDDVVTVDGKVIEKEEKEK
CCHHHHHHHHHHCCHHHHHHHHHHCCEEECCCEECCCEEECCCCEEEECCEEECCCCCCE
VYIAFHKPVGITCTAAKHIEGNIIAYINYPERIFPVGRLDKASEGLILLTNDGKIANQIL
EEEEEECCCCEEEEEEEECCCCEEEEEECCHHCCCCCCCCCCCCCEEEEECCCHHHHHHH
HGDHGHEKEYVVTVDKSFTDGFIHGMSCGVKIKDGMTKPCKVTRVDDSTFRIVLTQGMNR
CCCCCCCCCEEEEEECCCCCCEEEEEECCEEECCCCCCCEEEEEECCCEEEEEEECCCCH
QIRRMSRAFGYTVTKLKRVRIMNIKLGELEVGKWRYLTDEELQELRGAL
HHHHHHHHHCHHHHHEEEEEEEEEEECCEECCCEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]