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The map label for this gene is braB [H]

Identifier: 152976934

GI number: 152976934

Start: 3302137

End: 3303468

Strand: Reverse

Name: braB [H]

Synonym: Bcer98_3237

Alternate gene names: 152976934

Gene position: 3303468-3302137 (Counterclockwise)

Preceding gene: 152976935

Following gene: 152976933

Centisome position: 80.83

GC content: 36.56

Gene sequence:

>1332_bases
ATGAAAGTGAAGTCATCTTTAAAGGTATCTGAAATATTTGCAATTAGTTTAATGTTATTTGCACTTTTCTTTGGTGCAGG
AAATATGATTTTTCCACCAGCATTAGGACAAGGGGCTGGAACAAATGTATGGATTGCGTTATTTGGGTTTGTCATTACAG
GTGTGGGACTTCCGTTATTAGGAGTCATCGCAATCGCCTTAAAGGGAGATATTAATAAACTGGCCGGTCGTGTACATCCT
TTATTTGCACTTGTTTTCATTGTTGCGATTTATTTATGTTTAGGTGTATTTGTAAGTGTACCACGTACAGGCACAGTTGC
TTATGAAATGGCAGTTGCTCCATTTTTGCCAACTGAAGTAGCTGGTGCACAGTATCCACTTGTTATATTTACATTTATTT
TCTTTGCCGTTACATTTTATTTAGCATTGAATCCATCAAAACTAGTGGGACGCATCGGAAAAGTATTAACACCAATTTTA
TTAGCAATTATTGTGATTATTGTTGTGAAAGCATTGATTTCTCCAATTGGAGAGTTTCGTGCACCAACTGAAACTTATAA
AGACCCACTTTTCAAAGGATTTATTGATGGATATTTAACATTAGATGGATTAGCGGCGCTTGTTTTTGGAAATGTCGTTG
TGAATACGTTAAAAGGAAAAGGTGTTACAGATAAGAAAAGGATTGCACGAGTGACAATTATTGCAGGGATTGTTGCTGCG
CTTGCCTTATTTCTTATTTACTTAGCGCTTGCTTATTTAGGAGCGTCTAGTGTTTCTCTTGGAATGGGGGAGAACGGTGG
TGTTATTTTAACGAATATTGTTCATCATTTATTTGGTGAATATGGAACTTTATTACTAGGTGTAGCAATCACAGCAGCAT
GTTTAACAACATCTGTTGGAATCGTTGCAGCGTGCGGAGAGTTCTTTTCAAAACTATTACCAAAATTTCCTTATCAAACA
GTCGTTTTTATTTTCTGTGCATTAGCATTTATGGTGGCAAATCTTGGGTTAACACAGCTCATTGCATTAGCATTACCAAT
TTTAATTACATTGTATCCAATTGGGATTGTGTTGATTATCTTATCTCTTGTGGAAAATTATATTCGGATTCCATTAGCGA
TGTACGTAGGAGGGATGATAGGAGCGTTTGCAATTAGTCTGTTCGATGGTTTGCACAATGCGAATATTGAGATTGCAACA
CTTGCACCTATTTTAGAAAAAATACCACTTTATAGCGTTGGAATGGGCTGGCTAATTCCAGGAATCATTGGTATGTTTGT
CGGATATGTTGTGTCTATGTTTCAAAAGAGGGCAGTTGTGATAGAGGAGTAA

Upstream 100 bases:

>100_bases
GGACGGGTGTTTTTTGCGTTCTTTTGTAAAGAAAGTACGATATGGAAAAGGGGATGTAATGAATTTTGTTTCTGAATATT
CTGTTTATTTAGGGAGGTAA

Downstream 100 bases:

>100_bases
GCGACGAGAGTATTCTGAAAAAGGAGCTCGTTGTACTTACAAAGTGATAATATGGTATAGTGAAACGAGGCTTTTTTTCA
GCCTCGTTTTTCGTGCTTCC

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKVKSSLKVSEIFAISLMLFALFFGAGNMIFPPALGQGAGTNVWIALFGFVITGVGLPLLGVIAIALKGDINKLAGRVHP
LFALVFIVAIYLCLGVFVSVPRTGTVAYEMAVAPFLPTEVAGAQYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPIL
LAIIVIIVVKALISPIGEFRAPTETYKDPLFKGFIDGYLTLDGLAALVFGNVVVNTLKGKGVTDKKRIARVTIIAGIVAA
LALFLIYLALAYLGASSVSLGMGENGGVILTNIVHHLFGEYGTLLLGVAITAACLTTSVGIVAACGEFFSKLLPKFPYQT
VVFIFCALAFMVANLGLTQLIALALPILITLYPIGIVLIILSLVENYIRIPLAMYVGGMIGAFAISLFDGLHNANIEIAT
LAPILEKIPLYSVGMGWLIPGIIGMFVGYVVSMFQKRAVVIEE

Sequences:

>Translated_443_residues
MKVKSSLKVSEIFAISLMLFALFFGAGNMIFPPALGQGAGTNVWIALFGFVITGVGLPLLGVIAIALKGDINKLAGRVHP
LFALVFIVAIYLCLGVFVSVPRTGTVAYEMAVAPFLPTEVAGAQYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPIL
LAIIVIIVVKALISPIGEFRAPTETYKDPLFKGFIDGYLTLDGLAALVFGNVVVNTLKGKGVTDKKRIARVTIIAGIVAA
LALFLIYLALAYLGASSVSLGMGENGGVILTNIVHHLFGEYGTLLLGVAITAACLTTSVGIVAACGEFFSKLLPKFPYQT
VVFIFCALAFMVANLGLTQLIALALPILITLYPIGIVLIILSLVENYIRIPLAMYVGGMIGAFAISLFDGLHNANIEIAT
LAPILEKIPLYSVGMGWLIPGIIGMFVGYVVSMFQKRAVVIEE
>Mature_443_residues
MKVKSSLKVSEIFAISLMLFALFFGAGNMIFPPALGQGAGTNVWIALFGFVITGVGLPLLGVIAIALKGDINKLAGRVHP
LFALVFIVAIYLCLGVFVSVPRTGTVAYEMAVAPFLPTEVAGAQYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPIL
LAIIVIIVVKALISPIGEFRAPTETYKDPLFKGFIDGYLTLDGLAALVFGNVVVNTLKGKGVTDKKRIARVTIIAGIVAA
LALFLIYLALAYLGASSVSLGMGENGGVILTNIVHHLFGEYGTLLLGVAITAACLTTSVGIVAACGEFFSKLLPKFPYQT
VVFIFCALAFMVANLGLTQLIALALPILITLYPIGIVLIILSLVENYIRIPLAMYVGGMIGAFAISLFDGLHNANIEIAT
LAPILEKIPLYSVGMGWLIPGIIGMFVGYVVSMFQKRAVVIEE

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=424, Percent_Identity=37.5, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47072; Mature: 47072

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]