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The map label for this gene is hemL2 [H]

Identifier: 152976878

GI number: 152976878

Start: 3228575

End: 3229864

Strand: Reverse

Name: hemL2 [H]

Synonym: Bcer98_3177

Alternate gene names: 152976878

Gene position: 3229864-3228575 (Counterclockwise)

Preceding gene: 152976879

Following gene: 152976877

Centisome position: 79.03

GC content: 39.77

Gene sequence:

>1290_bases
ATGAAAAAGCTTGATAAATCGATTAAAGCGTTTGAAGAAGCACAAGATTTAATGCCTGGTGGTGTAAATAGTCCAGTTCG
TGCATTTAAATCGGTTGGGATGAATCCATTATTTATGGAGCAAGGAAAAGGTTCTAAAGTATATGATATTGATGGAAATG
AGTACATTGACTACGTATTATCATGGGGACCGCTTATTCATGGACATGCCAATGAGCGTGTTGTAGAGTCGTTAAAAGCT
GTTGCTGAAAAAGGAACAAGCTTTGGAGCGCCAACAGAAATTGAAAATAAGTTAGCTCAGCTTGTGATTGAGCGCGTGCC
ATCTATTGAAATTGTGCGCATGGTAAACTCTGGAACAGAGGCAACGATGAGTGCACTTCGTTTAGCACGTGGCTATACAG
GACGTAATAAAATTTTGAAATTTATCGGTTGTTACCACGGTCATGGTGATTCTCTATTAATTAAAGCTGGCTCTGGCGTT
GCAACACTTGGTTTACCGGATAGCCCTGGTGTACCAGAAGGTGTAGCCCAAAATACAATTACAGTAGCTTATAATGATTT
AGAAAGTGTAAAATATGCTTTTGAACAATTTGGAGATGATATTGCTTGTGTAATTGTTGAACCTGTAGCAGGAAATATGG
GAGTTGTACCACCTATACCAGGATTTTTAGAAGGACTTCGTGAAGTAACTCAGCAATATGGGGCGCTGCTTATCTTTGAT
GAGGTAATGACTGGATTCCGCGTTGCATATAACTGTGGACAAGGATATTACGGTGTAACGCCTGATTTAACTTGCTTAGG
AAAAGTAATCGGTGGCGGTCTACCGGTAGGAGCATACGGTGGTAAAGCAGAAATTATGCGTCAAGTTGCGCCTAGTGGAC
CGATTTATCAAGCGGGTACGTTATCTGGTAACCCACTTGCCATGACAGCAGGATATGAAACATTAATACAGCTAACACCA
GAATGTTATAAAGAGTTTGAACGTAAAGCGGAAATGTTAGAAAATGGTTTGCGCCAAGCTGCTGAAAAACATGGAATACC
GCACCATATTAACCGAGCAGGTTCAATGATTGGTATTTTCTTTACGAATGAACGAGTGATTAACTATGATGCAGCAAAAA
CATCGAATTTAGAGTTTTTTGCGGCTTATTATCGTGAAATGATAGAACAAGGGGTGTTTTTACCACCATCTCAATTTGAA
GGATTATTCTTATCAACAGCACATAGTGATGCTGATATTGAAGCGACAATTGCTGCGGCAGAAATTGCAATGGCGAAATT
AAAAGCATAA

Upstream 100 bases:

>100_bases
TTGAAAAATTATTAAGTATGAAACGTGCGGGAGCAGATTTAATTATTACGTATCATGCAAAAGATGCCGCAAGATGGTTA
CGAGAAGGGGGCGCACAATA

Downstream 100 bases:

>100_bases
ATAAAAAGGTTGTTTGGAATTTTCCAGGCAACCTTTTTCATTTATAAGAAATGAAAAGGGCACATGTGCGATTTTTTTAC
TCATAAAATGTCTCCAAGAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2 [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKKLDKSIKAFEEAQDLMPGGVNSPVRAFKSVGMNPLFMEQGKGSKVYDIDGNEYIDYVLSWGPLIHGHANERVVESLKA
VAEKGTSFGAPTEIENKLAQLVIERVPSIEIVRMVNSGTEATMSALRLARGYTGRNKILKFIGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGVAQNTITVAYNDLESVKYAFEQFGDDIACVIVEPVAGNMGVVPPIPGFLEGLREVTQQYGALLIFD
EVMTGFRVAYNCGQGYYGVTPDLTCLGKVIGGGLPVGAYGGKAEIMRQVAPSGPIYQAGTLSGNPLAMTAGYETLIQLTP
ECYKEFERKAEMLENGLRQAAEKHGIPHHINRAGSMIGIFFTNERVINYDAAKTSNLEFFAAYYREMIEQGVFLPPSQFE
GLFLSTAHSDADIEATIAAAEIAMAKLKA

Sequences:

>Translated_429_residues
MKKLDKSIKAFEEAQDLMPGGVNSPVRAFKSVGMNPLFMEQGKGSKVYDIDGNEYIDYVLSWGPLIHGHANERVVESLKA
VAEKGTSFGAPTEIENKLAQLVIERVPSIEIVRMVNSGTEATMSALRLARGYTGRNKILKFIGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGVAQNTITVAYNDLESVKYAFEQFGDDIACVIVEPVAGNMGVVPPIPGFLEGLREVTQQYGALLIFD
EVMTGFRVAYNCGQGYYGVTPDLTCLGKVIGGGLPVGAYGGKAEIMRQVAPSGPIYQAGTLSGNPLAMTAGYETLIQLTP
ECYKEFERKAEMLENGLRQAAEKHGIPHHINRAGSMIGIFFTNERVINYDAAKTSNLEFFAAYYREMIEQGVFLPPSQFE
GLFLSTAHSDADIEATIAAAEIAMAKLKA
>Mature_429_residues
MKKLDKSIKAFEEAQDLMPGGVNSPVRAFKSVGMNPLFMEQGKGSKVYDIDGNEYIDYVLSWGPLIHGHANERVVESLKA
VAEKGTSFGAPTEIENKLAQLVIERVPSIEIVRMVNSGTEATMSALRLARGYTGRNKILKFIGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGVAQNTITVAYNDLESVKYAFEQFGDDIACVIVEPVAGNMGVVPPIPGFLEGLREVTQQYGALLIFD
EVMTGFRVAYNCGQGYYGVTPDLTCLGKVIGGGLPVGAYGGKAEIMRQVAPSGPIYQAGTLSGNPLAMTAGYETLIQLTP
ECYKEFERKAEMLENGLRQAAEKHGIPHHINRAGSMIGIFFTNERVINYDAAKTSNLEFFAAYYREMIEQGVFLPPSQFE
GLFLSTAHSDADIEATIAAAEIAMAKLKA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=303, Percent_Identity=30.6930693069307, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI13994255, Length=321, Percent_Identity=28.6604361370716, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI24119277, Length=385, Percent_Identity=26.2337662337662, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI284507298, Length=222, Percent_Identity=32.4324324324324, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37574042, Length=354, Percent_Identity=24.5762711864407, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226442705, Length=353, Percent_Identity=24.3626062322946, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226442709, Length=275, Percent_Identity=26.1818181818182, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=56.8075117370892, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1789759, Length=329, Percent_Identity=32.8267477203647, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789016, Length=343, Percent_Identity=29.7376093294461, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788044, Length=356, Percent_Identity=32.3033707865169, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693181, Length=340, Percent_Identity=30.5882352941176, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787560, Length=376, Percent_Identity=26.5957446808511, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786991, Length=289, Percent_Identity=25.6055363321799, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=305, Percent_Identity=28.5245901639344, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=397, Percent_Identity=25.9445843828715, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=34.965034965035, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=313, Percent_Identity=27.4760383386581, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324432, Length=349, Percent_Identity=27.7936962750716, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357415, Length=313, Percent_Identity=27.4760383386581, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46238; Mature: 46238

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLDKSIKAFEEAQDLMPGGVNSPVRAFKSVGMNPLFMEQGKGSKVYDIDGNEYIDYVL
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLIHGHANERVVESLKAVAEKGTSFGAPTEIENKLAQLVIERVPSIEIVRMVNSGTE
HCCCEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCHH
ATMSALRLARGYTGRNKILKFIGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGVAQNTI
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVAYNDLESVKYAFEQFGDDIACVIVEPVAGNMGVVPPIPGFLEGLREVTQQYGALLIFD
EEEECHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEH
EVMTGFRVAYNCGQGYYGVTPDLTCLGKVIGGGLPVGAYGGKAEIMRQVAPSGPIYQAGT
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGYETLIQLTPECYKEFERKAEMLENGLRQAAEKHGIPHHINRAGSMIGIF
CCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEE
FTNERVINYDAAKTSNLEFFAAYYREMIEQGVFLPPSQFEGLFLSTAHSDADIEATIAAA
EECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
EIAMAKLKA
HHHHHHHCC
>Mature Secondary Structure
MKKLDKSIKAFEEAQDLMPGGVNSPVRAFKSVGMNPLFMEQGKGSKVYDIDGNEYIDYVL
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLIHGHANERVVESLKAVAEKGTSFGAPTEIENKLAQLVIERVPSIEIVRMVNSGTE
HCCCEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCHH
ATMSALRLARGYTGRNKILKFIGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGVAQNTI
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVAYNDLESVKYAFEQFGDDIACVIVEPVAGNMGVVPPIPGFLEGLREVTQQYGALLIFD
EEEECHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEH
EVMTGFRVAYNCGQGYYGVTPDLTCLGKVIGGGLPVGAYGGKAEIMRQVAPSGPIYQAGT
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGYETLIQLTPECYKEFERKAEMLENGLRQAAEKHGIPHHINRAGSMIGIF
CCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEE
FTNERVINYDAAKTSNLEFFAAYYREMIEQGVFLPPSQFEGLFLSTAHSDADIEATIAAA
EECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
EIAMAKLKA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA