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The map label for this gene is nadB [H]

Identifier: 152976846

GI number: 152976846

Start: 3193916

End: 3195424

Strand: Reverse

Name: nadB [H]

Synonym: Bcer98_3145

Alternate gene names: 152976846

Gene position: 3195424-3193916 (Counterclockwise)

Preceding gene: 152976850

Following gene: 152976845

Centisome position: 78.18

GC content: 39.76

Gene sequence:

>1509_bases
ATGCCAAGTGCAGATGTTTTAATTATTGGAAGTGGCATTGCGGCATTCCGTGTTGCAAAAGAGATTTGTCGTAAAAAAAA
TGTGATAATCCTTACAAAGAAAACGCGTCGCAATAACAATACATATTTAGCGCAAGGAGGAATTGCTGCAGCAATTGCTA
CATATGACAATCCAAATCATCATTATGAAGATACGTTAAAAGCAGGTTGTCATTATAACAATACAGAAGCTGTGCGTTAC
TTAGTGGAGAATGGACCAAAAGAAATTCACGAGCTCCTTGCAGCTGGTATGCAGTTTGATGGCGATGAAAAGGGACTCCA
CCTCGGGAAAGAAGGTGCGCATCGAAAACGACGGATTTTACATGCGGGAGGAGATACGACAGGGAAATATTTATTAGAGC
ATTTCATTCAAGAAATAACACCGCATGTTACAGTGATAGAACAAGAAAGAGTACTCGATTTCATCATAGAAAATGGGACG
TGTAGAGGCGTTTTAACACGTGATCAAAAAGGACATATGCATCGTTATTTTGCTGAATATACAGTATTAGCAACAGGAGG
CATAGGGGGATTATACACGTTTACTTCCAATGATGAAACGATAACAGGTGATGGAATTGCAATGGTATATCGAGCTGGTG
GTGAGCTTCAAGATTTAGAGTTTATGCAGTTTCACCCTACGATGTTACATGTAGATGGTAGGTGCTGTGGACTTGTTTCT
GAAGCTGTACGAGGCGAAGGAGCTATTCTTGTAAATGGGAAAGGAGAACATTTCATGACCGGAATACATCCGCAGCAAGA
TTTAGCACCGCGTGATGTAGTAGCCCGTGCGATCCATAGTCAGCTTCTCGTTGGTGAAGAAGTATATTTAGATATTTCAT
CTATTCAACATTTTGAAGACCGTTTTCCAACTGTTTCTTCATTATGTCGGATACATGGTGTGAATCTAAAACAAAATCGA
ATTCCAGTTGTACCGGGTGCACATTTTCATATGGGGGGTGTCAAAACAAACTGTGATGGGGAAACATCCATTCCACGTCT
ATATGCTATAGGAGAAACTGCTTGTAATGGTGTTCATGGTGCAAACCGACTAGCGAGCAATTCGTTGCTTGAGGGTCTTG
TATTTGGGAAAAGAATTGGACAGCATATTTTACACAATTCGGTAACAAGGGAAAAGTTTGATAGAGCAGAGCGGGATCAA
AGTCATCAAATCTTATATTTACCAACCAAAAGAGAAATACAAGAACAGATGATGAGGTATGTTGGAATTGTGCGTACAGA
GCAAGGGTTATTGCAAGCGAAAAAATGGTTTGAGCATTATGGTGCGCCTCATTTTGTATTACAATACGAGGCTCTTACAA
ATGAAGAAATTACGATTGTCAATATGTTAACAGTTTGCTGGCTAATGGTAGATGCGGCTTTGCAAAGGAAAGAAAGCATA
GGTGGACATTATCGCAGTGATGATCCAAAAAGAAGTGCAGTAGCAAAAGAAATGATGTTAATAAAATGA

Upstream 100 bases:

>100_bases
TTGTTATGAATAACTTCGAAAAAACTCTTGTCATTATTTTAAAATTATGTAAATATAGGTGTCAAGACAATTGTAATGTG
TAAATTAGGAGGCAATAATT

Downstream 100 bases:

>100_bases
CAAGCACGTTTGCATGAGGGAGAGAAGATAATGAACATATTAAAAGTACAGGAAGCATTAAAACAATTTTTTCTAGAAGA
TATAGGGGAATGGGATATAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPSADVLIIGSGIAAFRVAKEICRKKNVIILTKKTRRNNNTYLAQGGIAAAIATYDNPNHHYEDTLKAGCHYNNTEAVRY
LVENGPKEIHELLAAGMQFDGDEKGLHLGKEGAHRKRRILHAGGDTTGKYLLEHFIQEITPHVTVIEQERVLDFIIENGT
CRGVLTRDQKGHMHRYFAEYTVLATGGIGGLYTFTSNDETITGDGIAMVYRAGGELQDLEFMQFHPTMLHVDGRCCGLVS
EAVRGEGAILVNGKGEHFMTGIHPQQDLAPRDVVARAIHSQLLVGEEVYLDISSIQHFEDRFPTVSSLCRIHGVNLKQNR
IPVVPGAHFHMGGVKTNCDGETSIPRLYAIGETACNGVHGANRLASNSLLEGLVFGKRIGQHILHNSVTREKFDRAERDQ
SHQILYLPTKREIQEQMMRYVGIVRTEQGLLQAKKWFEHYGAPHFVLQYEALTNEEITIVNMLTVCWLMVDAALQRKESI
GGHYRSDDPKRSAVAKEMMLIK

Sequences:

>Translated_502_residues
MPSADVLIIGSGIAAFRVAKEICRKKNVIILTKKTRRNNNTYLAQGGIAAAIATYDNPNHHYEDTLKAGCHYNNTEAVRY
LVENGPKEIHELLAAGMQFDGDEKGLHLGKEGAHRKRRILHAGGDTTGKYLLEHFIQEITPHVTVIEQERVLDFIIENGT
CRGVLTRDQKGHMHRYFAEYTVLATGGIGGLYTFTSNDETITGDGIAMVYRAGGELQDLEFMQFHPTMLHVDGRCCGLVS
EAVRGEGAILVNGKGEHFMTGIHPQQDLAPRDVVARAIHSQLLVGEEVYLDISSIQHFEDRFPTVSSLCRIHGVNLKQNR
IPVVPGAHFHMGGVKTNCDGETSIPRLYAIGETACNGVHGANRLASNSLLEGLVFGKRIGQHILHNSVTREKFDRAERDQ
SHQILYLPTKREIQEQMMRYVGIVRTEQGLLQAKKWFEHYGAPHFVLQYEALTNEEITIVNMLTVCWLMVDAALQRKESI
GGHYRSDDPKRSAVAKEMMLIK
>Mature_501_residues
PSADVLIIGSGIAAFRVAKEICRKKNVIILTKKTRRNNNTYLAQGGIAAAIATYDNPNHHYEDTLKAGCHYNNTEAVRYL
VENGPKEIHELLAAGMQFDGDEKGLHLGKEGAHRKRRILHAGGDTTGKYLLEHFIQEITPHVTVIEQERVLDFIIENGTC
RGVLTRDQKGHMHRYFAEYTVLATGGIGGLYTFTSNDETITGDGIAMVYRAGGELQDLEFMQFHPTMLHVDGRCCGLVSE
AVRGEGAILVNGKGEHFMTGIHPQQDLAPRDVVARAIHSQLLVGEEVYLDISSIQHFEDRFPTVSSLCRIHGVNLKQNRI
PVVPGAHFHMGGVKTNCDGETSIPRLYAIGETACNGVHGANRLASNSLLEGLVFGKRIGQHILHNSVTREKFDRAERDQS
HQILYLPTKREIQEQMMRYVGIVRTEQGLLQAKKWFEHYGAPHFVLQYEALTNEEITIVNMLTVCWLMVDAALQRKESIG
GHYRSDDPKRSAVAKEMMLIK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=32.6254826254826, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1788928, Length=522, Percent_Identity=36.0153256704981, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790597, Length=510, Percent_Identity=29.2156862745098, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786942, Length=526, Percent_Identity=30.7984790874525, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17550100, Length=521, Percent_Identity=31.2859884836852, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=521, Percent_Identity=31.0940499040307, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322701, Length=510, Percent_Identity=32.9411764705882, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322416, Length=523, Percent_Identity=32.5047801147228, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=26.0960334029228, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=472, Percent_Identity=25.8474576271186, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137288, Length=516, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655642, Length=516, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655647, Length=516, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24663005, Length=517, Percent_Identity=31.5280464216634, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56067; Mature: 55936

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSADVLIIGSGIAAFRVAKEICRKKNVIILTKKTRRNNNTYLAQGGIAAAIATYDNPNH
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCC
HYEDTLKAGCHYNNTEAVRYLVENGPKEIHELLAAGMQFDGDEKGLHLGKEGAHRKRRIL
CHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEE
HAGGDTTGKYLLEHFIQEITPHVTVIEQERVLDFIIENGTCRGVLTRDQKGHMHRYFAEY
ECCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHE
TVLATGGIGGLYTFTSNDETITGDGIAMVYRAGGELQDLEFMQFHPTMLHVDGRCCGLVS
EEEEECCCCEEEEEECCCCEECCCCEEEEEECCCCCCCCHHHEECCEEEEECCHHHHHHH
EAVRGEGAILVNGKGEHFMTGIHPQQDLAPRDVVARAIHSQLLVGEEVYLDISSIQHFED
HHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHH
RFPTVSSLCRIHGVNLKQNRIPVVPGAHFHMGGVKTNCDGETSIPRLYAIGETACNGVHG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCEEEEECHHHCCCCCH
ANRLASNSLLEGLVFGKRIGQHILHNSVTREKFDRAERDQSHQILYLPTKREIQEQMMRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
VGIVRTEQGLLQAKKWFEHYGAPHFVLQYEALTNEEITIVNMLTVCWLMVDAALQRKESI
HHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGHYRSDDPKRSAVAKEMMLIK
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSADVLIIGSGIAAFRVAKEICRKKNVIILTKKTRRNNNTYLAQGGIAAAIATYDNPNH
CCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCC
HYEDTLKAGCHYNNTEAVRYLVENGPKEIHELLAAGMQFDGDEKGLHLGKEGAHRKRRIL
CHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHEE
HAGGDTTGKYLLEHFIQEITPHVTVIEQERVLDFIIENGTCRGVLTRDQKGHMHRYFAEY
ECCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHE
TVLATGGIGGLYTFTSNDETITGDGIAMVYRAGGELQDLEFMQFHPTMLHVDGRCCGLVS
EEEEECCCCEEEEEECCCCEECCCCEEEEEECCCCCCCCHHHEECCEEEEECCHHHHHHH
EAVRGEGAILVNGKGEHFMTGIHPQQDLAPRDVVARAIHSQLLVGEEVYLDISSIQHFED
HHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHH
RFPTVSSLCRIHGVNLKQNRIPVVPGAHFHMGGVKTNCDGETSIPRLYAIGETACNGVHG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCEEEEECHHHCCCCCH
ANRLASNSLLEGLVFGKRIGQHILHNSVTREKFDRAERDQSHQILYLPTKREIQEQMMRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
VGIVRTEQGLLQAKKWFEHYGAPHFVLQYEALTNEEITIVNMLTVCWLMVDAALQRKESI
HHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGHYRSDDPKRSAVAKEMMLIK
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]