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The map label for this gene is secDF [H]

Identifier: 152976829

GI number: 152976829

Start: 3174611

End: 3176869

Strand: Reverse

Name: secDF [H]

Synonym: Bcer98_3127

Alternate gene names: 152976829

Gene position: 3176869-3174611 (Counterclockwise)

Preceding gene: 152976830

Following gene: 152976828

Centisome position: 77.73

GC content: 36.7

Gene sequence:

>2259_bases
ATGGCAAAGCGTAGTACAAGAATTGTCGCCTTTTTCCTCACTGTTTTATTAATCGGTGGGATTATTGGTGCGACAGGAAA
AAACATAACAAAAGGCATTAGTCTTGGGTTAGACCTTCGCGGTGGTTTTGAAATCTTGTATCAAGTAAAACCAGCAAAGA
AAGGGGATAAAATTGATCACGATGCACTTGTGAGTACAGTAGGTGCTTTGAATAACCGCGTCAATGTTCTTGGTGTAAGT
GAGCCGAACATTCAAATCGAAGGAAAAGATCGAATTCGTGTTCAGCTTGCTGGTGTAAAAGATCAACAAAAAGCACGTGA
AATTTTATCTACCCAAGCAAAGTTAACATTCCGTGATGTGAATGATAATCTTCTTATGGATGGGGCAGATTTAAAAGGCG
GAGGAGCAAAGCAAACATTTGATGAACAAGGACGACCAAGCGTTGGACTTACATTAAAAAGTGCTGACAAATTCCGCGAA
GTAACAGAAAAAATTTCTAAAATGCCACCACCAACAAACTTGATGGTAATTTGGCTTGACTTTGAAGAAGGTAAGGATTC
TTATAAAGCGGAATCTACAAAACCAAATCCAAAATTTTTATCAGCAGCAACAGTAAATCAAGTATTTAATCAAACGGAAG
TTTCTATTGTAGGTGGAAACTTTACAGTTGAAAGTGCAAAGCAGCTTTCATCTTTATTAAATGCAGGGGCACTTCCTGTT
GATTTAAAAGAAATGTATTCAACATCTGTTGGGGCGAAATTTGGTCAACAAGCATTAGAAGAAACAATTTTTGCTAGTGT
GATTGGCATAGCTCTTATTTTCTTATTCATGCTTGTATTCTATCGTTTACCAGGATTTATCGCAGTGATTATGCTAGGGC
TGTATATTTTTATGACGCTTCTTGTCTTTAACTGGATGCATGCTGTTCTTACATTGCCAGGTATTGCGGCTTTAGTGCTC
GGGGTTGGTATCGCTGTTGATGCGAATATTATTACGTACGAGAGATTGAAGGAAGAATTGCGAATTGGTAAATCCATGAT
GTCAGCATATCGCTCAGGTAACCATCGTTCCTTAGCGACAATTTTGGATGCAAATATTACAACCCTTGCAGCAGCCGGCG
TGCTATTTGTATATGGTACAAGCTCTGTACAAGGGTTTGCAACAAGTTTGATTGTAAGTATTTTTGTTGGTTTTATTACG
AACGTATTCGGTACTCGTTTCTTACTAGGATTACTTGTGAAGAGCCGTTATTTTGATAAAAAACCAGCTTACTTTGGTGT
GAAACAAAAAGAAATTATTCCATTGTCAAAAGGCGTTGAGCATGCACCAACAAAATTTGACCGCATTAACTTTGTAAGTA
TCGGTCACAAATTTTTATTATTCTCAATTGTAGTTGTTATTGCAGGAGCAATTATACTACCAATTTTCAAACTGAACTTA
GGTATTGACTTTGCAAGTGGTACACGTATGGACTTGCAATCAAAACAAACAATTAGTGTTTCTCAAGTTTATAAGGATTT
ACATGAATTGAAAATTGATGTGAAAGAAGAAGATATTGTACCAACAGGTGATGACAATAAAGGATTCGCTGTTCGTACAA
TTGGTGTTTTATCAAAAGAGGAAATCGCAAAAGCGAAAACATTCTTCCATGATAAATATGGTACAGAACCAAATGTAAGC
ACAGTTTCGCCAACAATCGGGAAGGAAATTGCACAAAATGCCTTCATCGCAGTTCTGATTGCTTCAGCGGTTATCATACT
ATATGTAAGCATTCGTTTCCGATTTACATATGCGGTATCTGCTGTCGCTGCATTACTCCATGATGCATTTGTAATGATTG
TGGTGTTTAGTATTTTCCGTTTGGAAGTAGATTTAACATTTATTGCAGCTGTCTTAACCATTATTGGTTATTCTATCAAT
GACTCGATTGTTACATTTGACAGAAACCGTGAGTTATACAAACAGAAAAAACGTATTCGCGATTCAAAAGATCTAGAAGA
AATTGTAAACGCGAGTATTCGTCAAACAATTGGTCGTTCTATTAATACAGTATTAACTGTGCTATTCCCTGTGATTGCAC
TGATGATTTTCGGTAGTGAATCGTTACGAAACTTCTCGTTTGCATTAATGATAGGATTAGTTGTAGGTACGTATTCTTCT
GTTTTCGTTGCATCCCAAATTTGGCTTATGCTTGAAAAGCGCCGTTTGAAAAAAGGAAAGAAAAAGATAGTAGCAGAAGA
ATCTAAACCACAAGTTTAA

Upstream 100 bases:

>100_bases
TACAGAAAGGAAGTCTACTAACTGGACAAATATATATGTGAATATTATTCCATATAAGATTACATTTCTTTATGATGTTA
GTAGCAGAAAGAAGGTACAT

Downstream 100 bases:

>100_bases
AATGAGAAAAATAAAAGATGATGTCTCTTTCGAGGCATCTCTTTTTTCGTCTATGGATACAATAAAGAGATATTTCCTTT
TATGTACCTAGCTAAAGGAA

Product: bifunctional preprotein translocase subunit SecD/SecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MAKRSTRIVAFFLTVLLIGGIIGATGKNITKGISLGLDLRGGFEILYQVKPAKKGDKIDHDALVSTVGALNNRVNVLGVS
EPNIQIEGKDRIRVQLAGVKDQQKAREILSTQAKLTFRDVNDNLLMDGADLKGGGAKQTFDEQGRPSVGLTLKSADKFRE
VTEKISKMPPPTNLMVIWLDFEEGKDSYKAESTKPNPKFLSAATVNQVFNQTEVSIVGGNFTVESAKQLSSLLNAGALPV
DLKEMYSTSVGAKFGQQALEETIFASVIGIALIFLFMLVFYRLPGFIAVIMLGLYIFMTLLVFNWMHAVLTLPGIAALVL
GVGIAVDANIITYERLKEELRIGKSMMSAYRSGNHRSLATILDANITTLAAAGVLFVYGTSSVQGFATSLIVSIFVGFIT
NVFGTRFLLGLLVKSRYFDKKPAYFGVKQKEIIPLSKGVEHAPTKFDRINFVSIGHKFLLFSIVVVIAGAIILPIFKLNL
GIDFASGTRMDLQSKQTISVSQVYKDLHELKIDVKEEDIVPTGDDNKGFAVRTIGVLSKEEIAKAKTFFHDKYGTEPNVS
TVSPTIGKEIAQNAFIAVLIASAVIILYVSIRFRFTYAVSAVAALLHDAFVMIVVFSIFRLEVDLTFIAAVLTIIGYSIN
DSIVTFDRNRELYKQKKRIRDSKDLEEIVNASIRQTIGRSINTVLTVLFPVIALMIFGSESLRNFSFALMIGLVVGTYSS
VFVASQIWLMLEKRRLKKGKKKIVAEESKPQV

Sequences:

>Translated_752_residues
MAKRSTRIVAFFLTVLLIGGIIGATGKNITKGISLGLDLRGGFEILYQVKPAKKGDKIDHDALVSTVGALNNRVNVLGVS
EPNIQIEGKDRIRVQLAGVKDQQKAREILSTQAKLTFRDVNDNLLMDGADLKGGGAKQTFDEQGRPSVGLTLKSADKFRE
VTEKISKMPPPTNLMVIWLDFEEGKDSYKAESTKPNPKFLSAATVNQVFNQTEVSIVGGNFTVESAKQLSSLLNAGALPV
DLKEMYSTSVGAKFGQQALEETIFASVIGIALIFLFMLVFYRLPGFIAVIMLGLYIFMTLLVFNWMHAVLTLPGIAALVL
GVGIAVDANIITYERLKEELRIGKSMMSAYRSGNHRSLATILDANITTLAAAGVLFVYGTSSVQGFATSLIVSIFVGFIT
NVFGTRFLLGLLVKSRYFDKKPAYFGVKQKEIIPLSKGVEHAPTKFDRINFVSIGHKFLLFSIVVVIAGAIILPIFKLNL
GIDFASGTRMDLQSKQTISVSQVYKDLHELKIDVKEEDIVPTGDDNKGFAVRTIGVLSKEEIAKAKTFFHDKYGTEPNVS
TVSPTIGKEIAQNAFIAVLIASAVIILYVSIRFRFTYAVSAVAALLHDAFVMIVVFSIFRLEVDLTFIAAVLTIIGYSIN
DSIVTFDRNRELYKQKKRIRDSKDLEEIVNASIRQTIGRSINTVLTVLFPVIALMIFGSESLRNFSFALMIGLVVGTYSS
VFVASQIWLMLEKRRLKKGKKKIVAEESKPQV
>Mature_751_residues
AKRSTRIVAFFLTVLLIGGIIGATGKNITKGISLGLDLRGGFEILYQVKPAKKGDKIDHDALVSTVGALNNRVNVLGVSE
PNIQIEGKDRIRVQLAGVKDQQKAREILSTQAKLTFRDVNDNLLMDGADLKGGGAKQTFDEQGRPSVGLTLKSADKFREV
TEKISKMPPPTNLMVIWLDFEEGKDSYKAESTKPNPKFLSAATVNQVFNQTEVSIVGGNFTVESAKQLSSLLNAGALPVD
LKEMYSTSVGAKFGQQALEETIFASVIGIALIFLFMLVFYRLPGFIAVIMLGLYIFMTLLVFNWMHAVLTLPGIAALVLG
VGIAVDANIITYERLKEELRIGKSMMSAYRSGNHRSLATILDANITTLAAAGVLFVYGTSSVQGFATSLIVSIFVGFITN
VFGTRFLLGLLVKSRYFDKKPAYFGVKQKEIIPLSKGVEHAPTKFDRINFVSIGHKFLLFSIVVVIAGAIILPIFKLNLG
IDFASGTRMDLQSKQTISVSQVYKDLHELKIDVKEEDIVPTGDDNKGFAVRTIGVLSKEEIAKAKTFFHDKYGTEPNVST
VSPTIGKEIAQNAFIAVLIASAVIILYVSIRFRFTYAVSAVAALLHDAFVMIVVFSIFRLEVDLTFIAAVLTIIGYSIND
SIVTFDRNRELYKQKKRIRDSKDLEEIVNASIRQTIGRSINTVLTVLFPVIALMIFGSESLRNFSFALMIGLVVGTYSSV
FVASQIWLMLEKRRLKKGKKKIVAEESKPQV

Specific function: Required for efficient translocation of secretory pre- proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane [H]

COG id: COG0342

COG function: function code U; Preprotein translocase subunit SecD

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the secD/secF family. SecF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786609, Length=397, Percent_Identity=31.9899244332494, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786610, Length=308, Percent_Identity=29.5454545454545, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005791
- InterPro:   IPR022813
- InterPro:   IPR022646
- InterPro:   IPR022645
- InterPro:   IPR005665 [H]

Pfam domain/function: PF07549 Sec_GG; PF02355 SecD_SecF [H]

EC number: NA

Molecular weight: Translated: 82612; Mature: 82481

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRSTRIVAFFLTVLLIGGIIGATGKNITKGISLGLDLRGGFEILYQVKPAKKGDKIDH
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEECCCCEEEEEEECCCCCCCCCCH
DALVSTVGALNNRVNVLGVSEPNIQIEGKDRIRVQLAGVKDQQKAREILSTQAKLTFRDV
HHHHHHHHHHCCCEEEEEECCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEC
NDNLLMDGADLKGGGAKQTFDEQGRPSVGLTLKSADKFREVTEKISKMPPPTNLMVIWLD
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEE
FEEGKDSYKAESTKPNPKFLSAATVNQVFNQTEVSIVGGNFTVESAKQLSSLLNAGALPV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHCCCCCCC
DLKEMYSTSVGAKFGQQALEETIFASVIGIALIFLFMLVFYRLPGFIAVIMLGLYIFMTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNWMHAVLTLPGIAALVLGVGIAVDANIITYERLKEELRIGKSMMSAYRSGNHRSLAT
HHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILDANITTLAAAGVLFVYGTSSVQGFATSLIVSIFVGFITNVFGTRFLLGLLVKSRYFDK
HHHCCHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPAYFGVKQKEIIPLSKGVEHAPTKFDRINFVSIGHKFLLFSIVVVIAGAIILPIFKLNL
CCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFASGTRMDLQSKQTISVSQVYKDLHELKIDVKEEDIVPTGDDNKGFAVRTIGVLSKE
CEEECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCHH
EIAKAKTFFHDKYGTEPNVSTVSPTIGKEIAQNAFIAVLIASAVIILYVSIRFRFTYAVS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAALLHDAFVMIVVFSIFRLEVDLTFIAAVLTIIGYSINDSIVTFDRNRELYKQKKRIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCC
DSKDLEEIVNASIRQTIGRSINTVLTVLFPVIALMIFGSESLRNFSFALMIGLVVGTYSS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VFVASQIWLMLEKRRLKKGKKKIVAEESKPQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKRSTRIVAFFLTVLLIGGIIGATGKNITKGISLGLDLRGGFEILYQVKPAKKGDKIDH
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEECCCCEEEEEEECCCCCCCCCCH
DALVSTVGALNNRVNVLGVSEPNIQIEGKDRIRVQLAGVKDQQKAREILSTQAKLTFRDV
HHHHHHHHHHCCCEEEEEECCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEC
NDNLLMDGADLKGGGAKQTFDEQGRPSVGLTLKSADKFREVTEKISKMPPPTNLMVIWLD
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEE
FEEGKDSYKAESTKPNPKFLSAATVNQVFNQTEVSIVGGNFTVESAKQLSSLLNAGALPV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHCCCCCCC
DLKEMYSTSVGAKFGQQALEETIFASVIGIALIFLFMLVFYRLPGFIAVIMLGLYIFMTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNWMHAVLTLPGIAALVLGVGIAVDANIITYERLKEELRIGKSMMSAYRSGNHRSLAT
HHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILDANITTLAAAGVLFVYGTSSVQGFATSLIVSIFVGFITNVFGTRFLLGLLVKSRYFDK
HHHCCHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPAYFGVKQKEIIPLSKGVEHAPTKFDRINFVSIGHKFLLFSIVVVIAGAIILPIFKLNL
CCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFASGTRMDLQSKQTISVSQVYKDLHELKIDVKEEDIVPTGDDNKGFAVRTIGVLSKE
CEEECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCHH
EIAKAKTFFHDKYGTEPNVSTVSPTIGKEIAQNAFIAVLIASAVIILYVSIRFRFTYAVS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAALLHDAFVMIVVFSIFRLEVDLTFIAAVLTIIGYSINDSIVTFDRNRELYKQKKRIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCC
DSKDLEEIVNASIRQTIGRSINTVLTVLFPVIALMIFGSESLRNFSFALMIGLVVGTYSS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VFVASQIWLMLEKRRLKKGKKKIVAEESKPQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9694879; 9384377 [H]