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The map label for this gene is mccA [H]

Identifier: 152976787

GI number: 152976787

Start: 3127898

End: 3128821

Strand: Reverse

Name: mccA [H]

Synonym: Bcer98_3085

Alternate gene names: 152976787

Gene position: 3128821-3127898 (Counterclockwise)

Preceding gene: 152976788

Following gene: 152976786

Centisome position: 76.55

GC content: 38.31

Gene sequence:

>924_bases
ATGAAGGTGTATCGTGGGGTTCATGAATTAATTGGTCATACACCGATGGTAGAAATTACTCGTTTTTCACTTCCAGATGG
TGTTCGATTATTTGCGAAACTTGAATTTTATAATCCAGGTGGAAGTGTGAAAGATCGTCTCGGAAAAGAATTAATTGAAG
ATGCATTAGCGAAAGGGCTTGTCACAAAAGGTGGAACGCTTATTGAGCCAACAGCAGGGAATACTGGTATTGGACTGGCA
CTTGCTGCACTAGAGCATGGCTTACATGTTATTGTTTGTGTGCCAGAGAAATTTAGCATTGAAAAACAAGAGCTAATGAA
AGCACTTGGTGCAAAAGTTGTTCACACACCAACTGAACAAGGAATGACAGGGGCAATTGAAAAAGCAAAAGAGTTAGTCA
AAGAAATTCCAAATTCATATTCTCCAAGTCAGTTTGCTAACGAGGCAAACCCACGTGCTTATTTTAAAACACTGGGACCT
GAAATTTGGGAAGCTTTAAACGGAGAAGTAGATATATTTGTAGCAGGTGCAGGTACTGGTGGTACATTTATGGGTACTGC
ATCGTATTTAAAAGAACAAAATTCCAATGTAAAAACGGTTATTGTAGAACCAGAAGGATCTATTTTAAATGGTGGTGAAG
CAGGTTCACATGAAACGGAAGGGATTGGTCTTGAATTTATTCCGCCGTTTTTAAAGCCGTCTTACTTTAATGAAATTTAT
ACGATTTCTGATAAACATGCCTTTAAACGTGTGAAAGAATTAGCACAAAAAGAAGGACTCCTTGTAGGAAGTTCATCAGG
CGCTGCATTTCATGCAAGTTTGCTAGAAGCTGAGAAAGCAAAACCAGGCACAAATATTGTAACGATCTTTCCAGATAGCA
GTGAGCGTTACTTAAGTAAAGATATATACAAAGGATGGGAATAA

Upstream 100 bases:

>100_bases
ATTGAAAGAGTTAAAGTAATGTAACAAAAAGGCAACTGTTTCATATAAAGAATTACATACAGTTGCCTTTTCTTTATTGG
CAAGTAAGGAGGAGAACACG

Downstream 100 bases:

>100_bases
AAATGAGAGCAAAAACAAAATTAATTCATGGTATTCGCATAGGAGAACCTTCAACTGGATCTGTCAATGTGCCCATTTAT
CAAACAAGTACGTACAGACA

Product: pyridoxal-5'-phosphate-dependent protein beta subunit

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKVYRGVHELIGHTPMVEITRFSLPDGVRLFAKLEFYNPGGSVKDRLGKELIEDALAKGLVTKGGTLIEPTAGNTGIGLA
LAALEHGLHVIVCVPEKFSIEKQELMKALGAKVVHTPTEQGMTGAIEKAKELVKEIPNSYSPSQFANEANPRAYFKTLGP
EIWEALNGEVDIFVAGAGTGGTFMGTASYLKEQNSNVKTVIVEPEGSILNGGEAGSHETEGIGLEFIPPFLKPSYFNEIY
TISDKHAFKRVKELAQKEGLLVGSSSGAAFHASLLEAEKAKPGTNIVTIFPDSSERYLSKDIYKGWE

Sequences:

>Translated_307_residues
MKVYRGVHELIGHTPMVEITRFSLPDGVRLFAKLEFYNPGGSVKDRLGKELIEDALAKGLVTKGGTLIEPTAGNTGIGLA
LAALEHGLHVIVCVPEKFSIEKQELMKALGAKVVHTPTEQGMTGAIEKAKELVKEIPNSYSPSQFANEANPRAYFKTLGP
EIWEALNGEVDIFVAGAGTGGTFMGTASYLKEQNSNVKTVIVEPEGSILNGGEAGSHETEGIGLEFIPPFLKPSYFNEIY
TISDKHAFKRVKELAQKEGLLVGSSSGAAFHASLLEAEKAKPGTNIVTIFPDSSERYLSKDIYKGWE
>Mature_307_residues
MKVYRGVHELIGHTPMVEITRFSLPDGVRLFAKLEFYNPGGSVKDRLGKELIEDALAKGLVTKGGTLIEPTAGNTGIGLA
LAALEHGLHVIVCVPEKFSIEKQELMKALGAKVVHTPTEQGMTGAIEKAKELVKEIPNSYSPSQFANEANPRAYFKTLGP
EIWEALNGEVDIFVAGAGTGGTFMGTASYLKEQNSNVKTVIVEPEGSILNGGEAGSHETEGIGLEFIPPFLKPSYFNEIY
TISDKHAFKRVKELAQKEGLLVGSSSGAAFHASLLEAEKAKPGTNIVTIFPDSSERYLSKDIYKGWE

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=316, Percent_Identity=40.5063291139241, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI295821200, Length=316, Percent_Identity=40.5063291139241, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI4557415, Length=316, Percent_Identity=40.5063291139241, Blast_Score=231, Evalue=6e-61,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=42.4920127795527, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=35.9060402684564, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=41.6107382550336, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17562970, Length=298, Percent_Identity=38.255033557047, Blast_Score=206, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=44.4816053511706, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=38.9078498293515, Blast_Score=204, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=298, Percent_Identity=36.9127516778523, Blast_Score=183, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=37.5, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=36.4779874213836, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=38.2075471698113, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71996324, Length=334, Percent_Identity=27.2455089820359, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=322, Percent_Identity=41.304347826087, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=34.3558282208589, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=40.1898734177215, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=40.1898734177215, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33137; Mature: 33137

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYRGVHELIGHTPMVEITRFSLPDGVRLFAKLEFYNPGGSVKDRLGKELIEDALAKGL
CCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VTKGGTLIEPTAGNTGIGLALAALEHGLHVIVCVPEKFSIEKQELMKALGAKVVHTPTEQ
HCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCCCC
GMTGAIEKAKELVKEIPNSYSPSQFANEANPRAYFKTLGPEIWEALNGEVDIFVAGAGTG
CCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHCCHHHHHHHCCCEEEEEEECCCC
GTFMGTASYLKEQNSNVKTVIVEPEGSILNGGEAGSHETEGIGLEFIPPFLKPSYFNEIY
CCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCHHCCHHHHCCEE
TISDKHAFKRVKELAQKEGLLVGSSSGAAFHASLLEAEKAKPGTNIVTIFPDSSERYLSK
EECHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
DIYKGWE
HHHCCCC
>Mature Secondary Structure
MKVYRGVHELIGHTPMVEITRFSLPDGVRLFAKLEFYNPGGSVKDRLGKELIEDALAKGL
CCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VTKGGTLIEPTAGNTGIGLALAALEHGLHVIVCVPEKFSIEKQELMKALGAKVVHTPTEQ
HCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCCCC
GMTGAIEKAKELVKEIPNSYSPSQFANEANPRAYFKTLGPEIWEALNGEVDIFVAGAGTG
CCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHCCHHHHHHHCCCEEEEEEECCCC
GTFMGTASYLKEQNSNVKTVIVEPEGSILNGGEAGSHETEGIGLEFIPPFLKPSYFNEIY
CCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCHHCCHHHHCCEE
TISDKHAFKRVKELAQKEGLLVGSSSGAAFHASLLEAEKAKPGTNIVTIFPDSSERYLSK
EECHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
DIYKGWE
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]