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The map label for this gene is putP [H]

Identifier: 152969598

GI number: 152969598

Start: 1191666

End: 1193174

Strand: Direct

Name: putP [H]

Synonym: KPN_01042

Alternate gene names: 152969598

Gene position: 1191666-1193174 (Clockwise)

Preceding gene: 152969595

Following gene: 152969599

Centisome position: 22.42

GC content: 57.26

Gene sequence:

>1509_bases
ATGGCTATTAGCACACCGATGCTGGTGACATTTATTGTTTATATTTTTGGTATGGTATTGATAGGGTTTATTGCCTGGCG
TTCGACCAAAAACTTCGATGATTATATTCTCGGGGGAAGGAGTCTGGGGCCATTTGTGACCGCGCTTTCCGCAGGGGCGT
CGGATATGAGCGGCTGGCTGCTGATGGGCCTGCCAGGGGCGATTTTTCTCTCCGGGATTTCCGAAAGCTGGATCGCCATT
GGCTTAACGCTGGGGGCGTGGATTAACTGGAAGCTGGTGGCCGGTCGCCTGCGCGTGCATACCGAGGTGAATAATAACGC
CCTGACGCTGCCCGATTATTTTACCGGCCGTTTTGAAGATAAAAGCCGGGTGCTGAGAATTATTTCGGCGCTGGTTATTC
TGCTGTTCTTTACCATCTATTGCGCTTCAGGAATTGTCGCGGGGGCGCGTCTGTTTGAAAGCACCTTCGGCATGAGTTAT
GAAACCGCACTGTGGGCCGGGGCGGCGGCGACGATTATCTATACCTTCGTCGGCGGCTTCCTCGCGGTTAGCTGGACCGA
TACCGTGCAGGCCAGCCTGATGATTTTTGCGCTGATCCTCACCCCGGTGATCGTGATTATTTCCGTCGGCGGCTTTGGCG
ATTCGCTGGAAGTGATTAAGCAGAAGAGCATCGAAAATATCGACATGCTGAAAGGGCTGAACTTCGTGGCGATTATCTCG
CTGATGGGCTGGGGGCTGGGTTATTTCGGTCAGCCGCATATTCTGGCGCGCTTTATGGCGGCGGATTCGCACCACAGCAT
CGTTCACGCACGTCGCATTAGTATGACCTGGATGATCCTCTGCCTCGGCGGGGCGGTGGCCGTCGGCTTCTTTGGCATCG
CCTACTTCAATAACAACCCGAGCCTGGCGGGCGCGGTTAACCAGAACGCCGAGCGCGTGTTTATCGAACTGGCGCAAATC
CTGTTTAACCCGTGGATTGCCGGGATCCTGCTGTCGGCGATTCTGGCGGCGGTGATGTCCACCCTGAGCTGTCAGCTGCT
GGTCTGCTCGAGCGCAATTACCGAAGACCTGTATAAAGCCTTCCTGCGTAAAAACGCCGGTCAGAAAGAGCTGGTGTGGG
TGGGCCGTATGATGGTGCTGGTGGTTGCCCTGGTGGCCATTGCCCTGGCGGCCAATCCGGAAAACCGCGTGCTGGGTCTG
GTGAGCTACGCCTGGGCTGGCTTCGGCGCCGCCTTTGGCCCGGTGGTCCTGTTCTCGGTAATGTGGTCGCGCATGACCCG
TAACGGCGCGCTGGCCGGGATGGTGATTGGCGCCCTGACCGTGATCGTCTGGAAGCAGTTTGGCTGGCTGGGCCTGTATG
AAATCATTCCGGGTTTTGTCTTCGGCAGCATCGGGATTGTGGTCTTTAGCCTGCTGGATAAAGCGCCGTCGGCCAGCATG
CAGCAGCGCTTTGCCGAAGCGGATGCCCACTACCATACGCCGCCGCCGGTACGCGCCACGGCTGAGTAA

Upstream 100 bases:

>100_bases
GATACGGTCGCCCAATTAAACAATGCGCCATTACCCCGTTCCGGTAGTGGAAAAAAACGACGAAGTTGGACGCTGTCGAT
AAAAATTCTGGAGACTTTGC

Downstream 100 bases:

>100_bases
GATCATCTAATGCTCACGAGCCCGCCGCTGGCGGGCTTTTTATTGCCTGGTGCCGTGCCGGAATATTATAACCCTTGCTA
ATTATCTGAAAATCCGCTGA

Product: major sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAISTPMLVTFIVYIFGMVLIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEVNNNALTLPDYFTGRFEDKSRVLRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATIIYTFVGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENIDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLGGAVAVGFFGIAYFNNNPSLAGAVNQNAERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKNAGQKELVWVGRMMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMVIGALTVIVWKQFGWLGLYEIIPGFVFGSIGIVVFSLLDKAPSASM
QQRFAEADAHYHTPPPVRATAE

Sequences:

>Translated_502_residues
MAISTPMLVTFIVYIFGMVLIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEVNNNALTLPDYFTGRFEDKSRVLRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATIIYTFVGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENIDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLGGAVAVGFFGIAYFNNNPSLAGAVNQNAERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKNAGQKELVWVGRMMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMVIGALTVIVWKQFGWLGLYEIIPGFVFGSIGIVVFSLLDKAPSASM
QQRFAEADAHYHTPPPVRATAE
>Mature_501_residues
AISTPMLVTFIVYIFGMVLIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAIG
LTLGAWINWKLVAGRLRVHTEVNNNALTLPDYFTGRFEDKSRVLRIISALVILLFFTIYCASGIVAGARLFESTFGMSYE
TALWAGAAATIIYTFVGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENIDMLKGLNFVAIISL
MGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLGGAVAVGFFGIAYFNNNPSLAGAVNQNAERVFIELAQIL
FNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKNAGQKELVWVGRMMVLVVALVAIALAANPENRVLGLV
SYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMVIGALTVIVWKQFGWLGLYEIIPGFVFGSIGIVVFSLLDKAPSASMQ
QRFAEADAHYHTPPPVRATAE

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=486, Percent_Identity=45.2674897119342, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI110835708, Length=494, Percent_Identity=23.6842105263158, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4507035, Length=374, Percent_Identity=22.9946524064171, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787251, Length=498, Percent_Identity=95.3815261044177, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=451, Percent_Identity=26.3858093126386, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87082237, Length=468, Percent_Identity=27.1367521367521, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115533094, Length=425, Percent_Identity=26.3529411764706, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221459584, Length=386, Percent_Identity=22.5388601036269, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221459588, Length=375, Percent_Identity=24.8, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645928, Length=365, Percent_Identity=26.3013698630137, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650192, Length=399, Percent_Identity=22.0551378446115, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24651741, Length=370, Percent_Identity=23.5135135135135, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221459582, Length=398, Percent_Identity=24.6231155778894, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221459586, Length=377, Percent_Identity=22.0159151193634, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54247; Mature: 54116

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISTPMLVTFIVYIFGMVLIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAIFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEVNNNALTLPDYFTGRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEECEEEEEEEECCCEEECCCHHCCCCCC
KSRVLRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIIYTFVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENIDMLKGLNFVAIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLGGAVAVGFFGIAYFNNNP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
SLAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKNAGQKELVWVGRMMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMVIGALTVIVWKQFGWLGLYEIIPGFVFGSIGIVVFSLLDKAPSASM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QQRFAEADAHYHTPPPVRATAE
HHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISTPMLVTFIVYIFGMVLIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAIFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEVNNNALTLPDYFTGRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEECEEEEEEEECCCEEECCCHHCCCCCC
KSRVLRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIIYTFVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENIDMLKGLNFVAIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLGGAVAVGFFGIAYFNNNP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
SLAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKNAGQKELVWVGRMMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMVIGALTVIVWKQFGWLGLYEIIPGFVFGSIGIVVFSLLDKAPSASM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QQRFAEADAHYHTPPPVRATAE
HHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]