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The map label for this gene is ywkE [H]

Identifier: 151222233

GI number: 151222233

Start: 2237415

End: 2238251

Strand: Reverse

Name: ywkE [H]

Synonym: NWMN_2021

Alternate gene names: 151222233

Gene position: 2238251-2237415 (Counterclockwise)

Preceding gene: 151222234

Following gene: 151222232

Centisome position: 77.75

GC content: 34.53

Gene sequence:

>837_bases
ATGGTGAATTATAAAGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTTGAACAAACACGAGCTGAATG
GTTAATGTTAGATGTATTTCAATGGACGCGTACGGACTTTGTAGTCCACATGCATGATGATATGCCGAAAGCGATGATTA
TGAAGTTCGACTTAGCATTACAACGTATGTTATTAGGAGAGCCTATACAGTATATAGTTGGCTTTGCCTCATTTTATGGT
AGAACGTTTGATGTAAACTCAAATTGTTTGATACCAAGACCTGAAACTGAAGAAGTAATGTTGCATTTCTTACAACAGTT
AGAAGATGATGCAACAATCGTAGATATCGGAACGGGTAGTGGTGTACTTGCAATTACTTTGAAATGTGAAAAGCCGGATT
TAAATGTTATTGCTACTGATATTTCACTTGAAGCAATGAATATGGCTCGTAATAATGCTGAGAAGCATCAATCACAAATA
CAATTTTTAACAGGGGATGCATTAAAGCCCTTAATTAATGAAGGTATCAAGTTGAACGGCTTGATATCTAATCCACCATA
TATAGATGAAAAAGATATGGTTACGATGTCTCCAACGGTTACGAGATTCGAACCACATCAGGCATTGTTTGCAGATAACC
ATGGATATGCTATTTATGAATCAATCATTGAAGATTTACCTCACGTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATT
GGTTACAATCAAGGTGAGGCACTTAAATCAATAATTTTAAATAAATTTCCTGACAAAAAAATCGACATTATTAAAGATAT
AAATGGCCACGATCGAATCGTCTCATTTAAATGGTAA

Upstream 100 bases:

>100_bases
ACGCTTCAAAAATTAGGGCAAATTATGGAAGGCCATTTAGAAGAAATTATAGATGCACTGACTTTATCAGAGCAGACAGA
TAAATTGAAAGAACTTAATA

Downstream 100 bases:

>100_bases
TTAGAAGTTATGCCTTTGCTATGATTAGTTAAGTGCATAGCTTTTTGCTTTATATTATGATAAATAAGAAAGGCGTGATT
AAGTTGGATACTAAAATTTG

Product: modification methylase HemK family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYG
RTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

Sequences:

>Translated_278_residues
MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYG
RTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
>Mature_278_residues
MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYG
RTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787463, Length=280, Percent_Identity=31.4285714285714, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI87082085, Length=210, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582226, Length=213, Percent_Identity=23.943661971831, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31731; Mature: 31731

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
HHHHHCCHHHHHHHHHHHCCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCC
GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
CEEEEEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCEEEEE
LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
EECCCCCCCCCCEEEECCCEEEECCCHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEE
GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
CCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
HHHHHCCHHHHHHHHHHHCCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCC
GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
CEEEEEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCEEEEE
LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
EECCCCCCCCCCEEEECCCEEEECCCHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEE
GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
CCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]