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The map label for this gene is gatD [H]

Identifier: 150401792

GI number: 150401792

Start: 1433254

End: 1434513

Strand: Direct

Name: gatD [H]

Synonym: Maeo_1370

Alternate gene names: 150401792

Gene position: 1433254-1434513 (Clockwise)

Preceding gene: 150401791

Following gene: 150401799

Centisome position: 91.32

GC content: 32.3

Gene sequence:

>1260_bases
ATGGATAATTATGATATTGGAGATTTAATTACAATAGAAACAGAAAATACTACATATACAGGCACTATTATGCCGTCATT
AAATGATAACATAATAGTTATTAAAATGAAAAGTGGATATAATGCAGGAATAGATAAAAATAAAATAAAATTAATAAAAT
TAATAAATAAAGGAGAAAAACCAAATTATAAAATTCCGCCGTTAAAAATATCAGAAAATAATAATTTAAAAAATATATCT
ATTCTATCAACGGGGGGGACAGTAGCTTCAAGAGTGGATTATAACACTGGTGCAGTTCATCCGGCTTTCACAGCAGATGA
TTTAATAAGGGCAGTGCCTGAATTATTGGATATTGCAAATATAAATGGTAAGGTGGTTTTAAACATATTAAGCGAAAATA
TGACACCAACCTATTGGAAAATGATAGCTGAAGAAATAGAAAAAGAAGTAAAAAACGGCGCAGATGGTATTGTAATAGCT
CATGGAACTGATACTATGCACTATACGGCATGTGCCTTATCATTCATGGTTGATGCTGATATTCCAATAGTTCTTGTTGG
GGCTCAGCGAAGTAGTGATAGACCTTCTTCAGACGCGGCACTAAATTTAATAAGTGCTACAATGTGCGCAACAGAGCAAA
TAAAAGGAGTTTTCGTAGTTATGCACGGTGAAAGCGGGGATACAGTTTGCCATCTCCATAGGGGCGTAAAAGTTAGGAAA
TCCCATTCAACTAGGAGAGATGCCTTTAAATCCATAAACTCTATTCCGGTGGCAGAAATAAACCCATTTAAAAAACAAAT
AAAATATTTGACAGAATTGGAAAAAACAAATAAAAATAATATTAAAATAAACACAAATTTGGAGGAAAAAGTAGCTTTAA
TAAAGATATATCCAGGAATAGATGGAAATGTCATAAATTATTATGTAGATAATGGATACAAAGGAATAATAATTGAAGGG
ACAGGATTGGGACACACACCAGAAACAATATTTGAAAATATAAAATATGCAACAGATAAAGGAGTTTTAGTGGCAATGAC
TACCCAGACAATAAATGGAAGAGTAAATATGAATGTTTATTCAAACGGCAGGGAGCTCCAAAAATTAGGGGTAATTAGCT
GTGAAGATATGCTTCCAGAAGTGGCATTAGTTAAAATGATGCATTTACTGGGCAACTACGACACAGAAAAGGCAAAAGAG
TTGATGAATAAAAATTTAGTAGGGGAAATAACAGAAATTAGCAGATTTGACAGTTATTAA

Upstream 100 bases:

>100_bases
AAAATATTATACAATAATTAAATAATATTAAAAAACCAAATAATTTATTTTTTCATATCTATTTTTTATATTTTTTAATT
TCAATTTTATTGGTGAAATA

Downstream 100 bases:

>100_bases
GATTTTTATTTTTATTTTTATTTTTTCCGTTATCTAACAAAAAACATCAATAAAATAACTCCAAATAATATTCTATATAT
TCCAAATGGCACAAAATTAT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDNYDIGDLITIETENTTYTGTIMPSLNDNIIVIKMKSGYNAGIDKNKIKLIKLINKGEKPNYKIPPLKISENNNLKNIS
ILSTGGTVASRVDYNTGAVHPAFTADDLIRAVPELLDIANINGKVVLNILSENMTPTYWKMIAEEIEKEVKNGADGIVIA
HGTDTMHYTACALSFMVDADIPIVLVGAQRSSDRPSSDAALNLISATMCATEQIKGVFVVMHGESGDTVCHLHRGVKVRK
SHSTRRDAFKSINSIPVAEINPFKKQIKYLTELEKTNKNNIKINTNLEEKVALIKIYPGIDGNVINYYVDNGYKGIIIEG
TGLGHTPETIFENIKYATDKGVLVAMTTQTINGRVNMNVYSNGRELQKLGVISCEDMLPEVALVKMMHLLGNYDTEKAKE
LMNKNLVGEITEISRFDSY

Sequences:

>Translated_419_residues
MDNYDIGDLITIETENTTYTGTIMPSLNDNIIVIKMKSGYNAGIDKNKIKLIKLINKGEKPNYKIPPLKISENNNLKNIS
ILSTGGTVASRVDYNTGAVHPAFTADDLIRAVPELLDIANINGKVVLNILSENMTPTYWKMIAEEIEKEVKNGADGIVIA
HGTDTMHYTACALSFMVDADIPIVLVGAQRSSDRPSSDAALNLISATMCATEQIKGVFVVMHGESGDTVCHLHRGVKVRK
SHSTRRDAFKSINSIPVAEINPFKKQIKYLTELEKTNKNNIKINTNLEEKVALIKIYPGIDGNVINYYVDNGYKGIIIEG
TGLGHTPETIFENIKYATDKGVLVAMTTQTINGRVNMNVYSNGRELQKLGVISCEDMLPEVALVKMMHLLGNYDTEKAKE
LMNKNLVGEITEISRFDSY
>Mature_419_residues
MDNYDIGDLITIETENTTYTGTIMPSLNDNIIVIKMKSGYNAGIDKNKIKLIKLINKGEKPNYKIPPLKISENNNLKNIS
ILSTGGTVASRVDYNTGAVHPAFTADDLIRAVPELLDIANINGKVVLNILSENMTPTYWKMIAEEIEKEVKNGADGIVIA
HGTDTMHYTACALSFMVDADIPIVLVGAQRSSDRPSSDAALNLISATMCATEQIKGVFVVMHGESGDTVCHLHRGVKVRK
SHSTRRDAFKSINSIPVAEINPFKKQIKYLTELEKTNKNNIKINTNLEEKVALIKIYPGIDGNVINYYVDNGYKGIIIEG
TGLGHTPETIFENIKYATDKGVLVAMTTQTINGRVNMNVYSNGRELQKLGVISCEDMLPEVALVKMMHLLGNYDTEKAKE
LMNKNLVGEITEISRFDSY

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=374, Percent_Identity=27.807486631016, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1788065, Length=286, Percent_Identity=32.8671328671329, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789327, Length=321, Percent_Identity=29.9065420560748, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17558278, Length=371, Percent_Identity=27.4932614555256, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI193207372, Length=371, Percent_Identity=27.4932614555256, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17558280, Length=371, Percent_Identity=27.4932614555256, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI193207374, Length=371, Percent_Identity=27.4932614555256, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320527, Length=351, Percent_Identity=25.3561253561254, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=308, Percent_Identity=26.9480519480519, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323187, Length=308, Percent_Identity=26.9480519480519, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323186, Length=308, Percent_Identity=26.9480519480519, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323184, Length=308, Percent_Identity=26.9480519480519, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24639609, Length=289, Percent_Identity=30.1038062283737, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24645530, Length=285, Percent_Identity=31.9298245614035, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 46246; Mature: 46246

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYDIGDLITIETENTTYTGTIMPSLNDNIIVIKMKSGYNAGIDKNKIKLIKLINKGEK
CCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCC
PNYKIPPLKISENNNLKNISILSTGGTVASRVDYNTGAVHPAFTADDLIRAVPELLDIAN
CCCCCCCEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
INGKVVLNILSENMTPTYWKMIAEEIEKEVKNGADGIVIAHGTDTMHYTACALSFMVDAD
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHEEEEEECCC
IPIVLVGAQRSSDRPSSDAALNLISATMCATEQIKGVFVVMHGESGDTVCHLHRGVKVRK
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCEEEEC
SHSTRRDAFKSINSIPVAEINPFKKQIKYLTELEKTNKNNIKINTNLEEKVALIKIYPGI
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHEEEEEEEECCC
DGNVINYYVDNGYKGIIIEGTGLGHTPETIFENIKYATDKGVLVAMTTQTINGRVNMNVY
CCCEEEEEEECCEEEEEEECCCCCCCHHHHHHCCEEECCCCEEEEEEEEEECCEEEEEEE
SNGRELQKLGVISCEDMLPEVALVKMMHLLGNYDTEKAKELMNKNLVGEITEISRFDSY
CCCCHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MDNYDIGDLITIETENTTYTGTIMPSLNDNIIVIKMKSGYNAGIDKNKIKLIKLINKGEK
CCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCC
PNYKIPPLKISENNNLKNISILSTGGTVASRVDYNTGAVHPAFTADDLIRAVPELLDIAN
CCCCCCCEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
INGKVVLNILSENMTPTYWKMIAEEIEKEVKNGADGIVIAHGTDTMHYTACALSFMVDAD
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHEEEEEECCC
IPIVLVGAQRSSDRPSSDAALNLISATMCATEQIKGVFVVMHGESGDTVCHLHRGVKVRK
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCEEEEC
SHSTRRDAFKSINSIPVAEINPFKKQIKYLTELEKTNKNNIKINTNLEEKVALIKIYPGI
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHEEEEEEEECCC
DGNVINYYVDNGYKGIIIEGTGLGHTPETIFENIKYATDKGVLVAMTTQTINGRVNMNVY
CCCEEEEEEECCEEEEEEECCCCCCCHHHHHHCCEEECCCCEEEEEEEEEECCEEEEEEE
SNGRELQKLGVISCEDMLPEVALVKMMHLLGNYDTEKAKELMNKNLVGEITEISRFDSY
CCCCHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA