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The map label for this gene is kdsB [H]

Identifier: 150398613

GI number: 150398613

Start: 3632689

End: 3633441

Strand: Direct

Name: kdsB [H]

Synonym: Smed_3427

Alternate gene names: 150398613

Gene position: 3632689-3633441 (Clockwise)

Preceding gene: 150398610

Following gene: 150398614

Centisome position: 96.05

GC content: 64.94

Gene sequence:

>753_bases
ATGAATCGTGAACAAGCGGGCAAAGCCCTCGTACTCATTCCGGCGCGCATGGCGTCGACGCGCCTGCCCGGCAAGCCGCT
TGCCGACATTTGCGGGCTGCCGATGATCGTGCAGGTGGCAAGGCGGGCGGCGGAGGCCGAGGTCGGCCGGATCGTGGTGG
CCGTGGATCATCCGGAGGTTTTCGCAGCCGTGAGCGACGCCGGCTTCGAGGCGATCATGACGCGGGTCGACCACCAGTCC
GGCTCCGACCGGATTCACGAGGCGCTTCTTAAGGCCGATCCGCAAGGTGAGGCGGAAATCGTCATCAACGTGCAGGGCGA
TTTGCCGACCATCGAGCCCGGCCCGATCCGTGCGGCACTGAAGCCGCTGGAAAATCCTTCGACGGACATTGCCACGCTGA
CCGTGGCGATCACCGACGAGCACGAAAAGACCAACCCCAACGTCGTCAAGGTCGTCGGCTCGCCGCTGTCGCAGAGCCGC
TTCCGCGCGCTCTATTTCACCCGCGCGACCGCGCCCTACGGCGAAGGTCCTCTCTATCACCATATCGGCCTTTACGCCTA
TCGCCGCAGGGCACTCGAGACCTTCGTTTCGTTGAAGCCGTCGGCGCTTGAAAAGCGCGAATCGCTGGAACAGCTGCGGG
CGCTCGAAGCGGGCATGCGCATCGATGTGGAGATCGTCGATTCGGTGCCGCTCGGCGTCGACACGCCTGCAGATCTCGAC
AAGGCCCGCCGGATCCTTTCGGCCCGGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCGGAACCTATGTCTTTTGGCTCTGCGTTGCAACAGAGATTATCAACCCCGGAGTGAGACGTTTTGACACTTTCTCC
GGGTTTCAAAAGGCCGGATC

Downstream 100 bases:

>100_bases
GATTTTCACATCAAGGGGAACTGACGTGACAGCCAAGACCAACCGGATTTCCTTTCAGGGCGACTACGGCGCCAATTCCG
ACATGGCCTGCCGCGACATG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNREQAGKALVLIPARMASTRLPGKPLADICGLPMIVQVARRAAEAEVGRIVVAVDHPEVFAAVSDAGFEAIMTRVDHQS
GSDRIHEALLKADPQGEAEIVINVQGDLPTIEPGPIRAALKPLENPSTDIATLTVAITDEHEKTNPNVVKVVGSPLSQSR
FRALYFTRATAPYGEGPLYHHIGLYAYRRRALETFVSLKPSALEKRESLEQLRALEAGMRIDVEIVDSVPLGVDTPADLD
KARRILSARA

Sequences:

>Translated_250_residues
MNREQAGKALVLIPARMASTRLPGKPLADICGLPMIVQVARRAAEAEVGRIVVAVDHPEVFAAVSDAGFEAIMTRVDHQS
GSDRIHEALLKADPQGEAEIVINVQGDLPTIEPGPIRAALKPLENPSTDIATLTVAITDEHEKTNPNVVKVVGSPLSQSR
FRALYFTRATAPYGEGPLYHHIGLYAYRRRALETFVSLKPSALEKRESLEQLRALEAGMRIDVEIVDSVPLGVDTPADLD
KARRILSARA
>Mature_250_residues
MNREQAGKALVLIPARMASTRLPGKPLADICGLPMIVQVARRAAEAEVGRIVVAVDHPEVFAAVSDAGFEAIMTRVDHQS
GSDRIHEALLKADPQGEAEIVINVQGDLPTIEPGPIRAALKPLENPSTDIATLTVAITDEHEKTNPNVVKVVGSPLSQSR
FRALYFTRATAPYGEGPLYHHIGLYAYRRRALETFVSLKPSALEKRESLEQLRALEAGMRIDVEIVDSVPLGVDTPADLD
KARRILSARA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=246, Percent_Identity=45.9349593495935, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27119; Mature: 27119

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREQAGKALVLIPARMASTRLPGKPLADICGLPMIVQVARRAAEAEVGRIVVAVDHPEV
CCCHHHCCEEEEEECHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCHH
FAAVSDAGFEAIMTRVDHQSGSDRIHEALLKADPQGEAEIVINVQGDLPTIEPGPIRAAL
EEHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHH
KPLENPSTDIATLTVAITDEHEKTNPNVVKVVGSPLSQSRFRALYFTRATAPYGEGPLYH
HCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCCHH
HIGLYAYRRRALETFVSLKPSALEKRESLEQLRALEAGMRIDVEIVDSVPLGVDTPADLD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
KARRILSARA
HHHHHHHCCC
>Mature Secondary Structure
MNREQAGKALVLIPARMASTRLPGKPLADICGLPMIVQVARRAAEAEVGRIVVAVDHPEV
CCCHHHCCEEEEEECHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCHH
FAAVSDAGFEAIMTRVDHQSGSDRIHEALLKADPQGEAEIVINVQGDLPTIEPGPIRAAL
EEHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHH
KPLENPSTDIATLTVAITDEHEKTNPNVVKVVGSPLSQSRFRALYFTRATAPYGEGPLYH
HCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCCHH
HIGLYAYRRRALETFVSLKPSALEKRESLEQLRALEAGMRIDVEIVDSVPLGVDTPADLD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
KARRILSARA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]