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The map label for this gene is pbpG [H]

Identifier: 150398577

GI number: 150398577

Start: 3591205

End: 3593457

Strand: Direct

Name: pbpG [H]

Synonym: Smed_3388

Alternate gene names: 150398577

Gene position: 3591205-3593457 (Clockwise)

Preceding gene: 150398573

Following gene: 150398578

Centisome position: 94.96

GC content: 65.25

Gene sequence:

>2253_bases
ATGGCGAGCGGCAAATCAGGACAGCGGATCGAGCCTTCCTTCGGCGGTAACGACGACGGCGGCGAGCTGCGGGTCGACGC
GAGCGATCGCGTGGCGGGCGGCGGACGCAAGCCGAAGCGCGCCAAGCCGCCCGAACGCGGCGCCAAGGGAGAAAAACGCG
CCAGGCGTGCCGGCGGGCGCGGCTCCGGCAAAAGCGGTTCCGGCGGGTTCTTCGGCCTCGCCCGGCGGTTCGCCTATTGG
TGCGTCGTGCTCGGCATATGGGGTGCGATCGGCGTCGGCGGGCTCGTGCTCTATTACGGAGCGCGCATGCCGAGCGCGAC
GAGCTGGTCGATCCCAGAGCGGCCGCCCAACGTCAAGATTGTCGCTGCCAATGGCGAGATCATCGCCAACAGGGGCGCGA
CAGGCGGAGAGGCTGTCGCGCTCGAAGACATGTCGCCCTATATCCCTCAGGCGGTGATCGCCATCGAGGACCGCCGATTC
CATTCGCATTTCGGCGTTGACCCGCTGGGGCTGGCGCGCGCCATGCTCACGAACGTCATGAGCGGCCGCATGGTCCAGGG
CGGCTCCACGCTCACCCAGCAGCTTGCCAAAAACCTGTTCCTCTCGCCGGAGCGCACGCTCGAGCGAAAGGTGCAGGAGG
TGCTGCTCGCTTTCTGGCTGGAGCAGAAATACACCAAGGACCAGATCCTGGCGATGTATCTCAACCGCGTCTTCTTCGGG
TCGAACGCCTATGGCGTCGAGGCGGCTTCGCGGCGCTACTTCAACAAGTCGGCGCGCGACGTCAATCTCGGCGAGGCGGC
ATTGCTCGCGGGCCTTCTGAAAGCCCCTTCACGGCTGTCGCCGGCCCGGAACCCCGAAGCAGCGGAGGAGCGCGCGCAGC
TCGTGCTTGGCGCCATGCGTGAGGAAGGCTTCATCAACGATGCCGAGATCAAGACGGCGATGTCGCAGGCGCCGACCCGG
GCAAAGAGCTTCTGGTCCGGCGCCGAACACTACGTCGCGGATATGGTTATGGACCAGCTCCCCGGCATGATCGGCGAAGT
TACACAAGACCTCGTCATCGACACGACCATCGACCTTGCGCTCGAGAAGAGGGCCGAAGAGATTCTCGCCGAAAGGCTTG
AGACGGAGGGCGGCAAGGTCAACGCCTCGCAGGCGGCGCTCGTCTCCCTCGACGGCACCGGTGCCATCCGGGCACTGGTT
GGCGGCAGGGACTATGCCGAAAGCCAGTTCGATCGCGCCTCCAAGGCCAAGCGGCAGCCAGGATCGGCCTTCAAGCCTTT
CGTCTATGCAGCGGCACTCGAGATCGGCCGCACGCCGATGTCCGTTCGCAACGACGTGCCTGTCAGGATCGGCAACTGGA
CGCCTGAAAACTATGACCAGAAATATCGAGGCGAGGTGACGCTCGCCGATGCGCTCGCCAATTCGCTGAACACCATCGCC
GCCCAGCTGGTGATGGAGGTCGGGCCGCAAAACGTGGTCAAGCTCGCGCACCGCCTCGGCATCGATTCCGAGATGCAGGC
CAATGCATCGATCGCGCTCGGCACGTCGGAAGTGACGCTGGTGGAGCTGACGTCCTCCTATGCGCCTTTCATGAATGGCG
GCTACAAGGCGACGCCGCACGTTATCCGGCGCATCTCTACCGCCGACGGCACCGTGCTTTACGAGAACACCTACGACAAT
CCGCCCCGGGTGCTCGATCCGGCGGTCGTCAGCGAGATGAACCGGATGATGGTGCGCGTGCTGACTGACGGCACCGGCAA
GAATGCACGTCTGCCGGGCTGGGAGGCAGCGGGAAAGACCGGAACGACGCAATCATTCCGCGACGCGCTCTTCGTCGGCT
ACACCTCCAGCCTCGCGACCGGTATCTGGTTCGGCAATGACGACGGCAAGTTCATGAAGAAGGTCACCGGCGGCGGCCTT
CCCGCCCGTGCCTGGCATGATTTCATGACGGCGGCGCATGAGGGCCTCTCGCCCTCGCCGCTCTTCGGCACGACGGGCGT
ACAGCCGGTTTTCGACCAAGGCATCGCGGCTGCGGAGGAGATGCCGGTTGACGGTTTCGATACCGGTAGCCCGGATGTTT
TTCCTGAGCGCCCGGCAGGTATGGATGGCATGGAAAGCATGGACGGCATGGCCGCCGCCGATCAGATCCGGCCGGCAGAA
CAGGCGGGAGGGCCGATTCCGCCGGCGGGCGTCGGCGAGACCACCGGAGGCACCCGCCGCACGACGCTTTTCGATCTGCT
GACCGGAGGCTGA

Upstream 100 bases:

>100_bases
CGGACGGGGCGGAGCCTCCCGCACGACAACGGCCCAGCCGGGAGCAGCTTGCGAGCGATGTTCACCCCGGTTCGGCCCTA
AATGGGTATTTCCTTCCGGC

Downstream 100 bases:

>100_bases
GGCAGCCGTCTGCGATGGCAGCCGGCGCGCGTCGGGCGTTTGCGAAAATGCGGCTTGCATTTGCCGCTGATAGCCCCGCA
GAATGCCTTGCAGTCCGAAA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MASGKSGQRIEPSFGGNDDGGELRVDASDRVAGGGRKPKRAKPPERGAKGEKRARRAGGRGSGKSGSGGFFGLARRFAYW
CVVLGIWGAIGVGGLVLYYGARMPSATSWSIPERPPNVKIVAANGEIIANRGATGGEAVALEDMSPYIPQAVIAIEDRRF
HSHFGVDPLGLARAMLTNVMSGRMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLAFWLEQKYTKDQILAMYLNRVFFG
SNAYGVEAASRRYFNKSARDVNLGEAALLAGLLKAPSRLSPARNPEAAEERAQLVLGAMREEGFINDAEIKTAMSQAPTR
AKSFWSGAEHYVADMVMDQLPGMIGEVTQDLVIDTTIDLALEKRAEEILAERLETEGGKVNASQAALVSLDGTGAIRALV
GGRDYAESQFDRASKAKRQPGSAFKPFVYAAALEIGRTPMSVRNDVPVRIGNWTPENYDQKYRGEVTLADALANSLNTIA
AQLVMEVGPQNVVKLAHRLGIDSEMQANASIALGTSEVTLVELTSSYAPFMNGGYKATPHVIRRISTADGTVLYENTYDN
PPRVLDPAVVSEMNRMMVRVLTDGTGKNARLPGWEAAGKTGTTQSFRDALFVGYTSSLATGIWFGNDDGKFMKKVTGGGL
PARAWHDFMTAAHEGLSPSPLFGTTGVQPVFDQGIAAAEEMPVDGFDTGSPDVFPERPAGMDGMESMDGMAAADQIRPAE
QAGGPIPPAGVGETTGGTRRTTLFDLLTGG

Sequences:

>Translated_750_residues
MASGKSGQRIEPSFGGNDDGGELRVDASDRVAGGGRKPKRAKPPERGAKGEKRARRAGGRGSGKSGSGGFFGLARRFAYW
CVVLGIWGAIGVGGLVLYYGARMPSATSWSIPERPPNVKIVAANGEIIANRGATGGEAVALEDMSPYIPQAVIAIEDRRF
HSHFGVDPLGLARAMLTNVMSGRMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLAFWLEQKYTKDQILAMYLNRVFFG
SNAYGVEAASRRYFNKSARDVNLGEAALLAGLLKAPSRLSPARNPEAAEERAQLVLGAMREEGFINDAEIKTAMSQAPTR
AKSFWSGAEHYVADMVMDQLPGMIGEVTQDLVIDTTIDLALEKRAEEILAERLETEGGKVNASQAALVSLDGTGAIRALV
GGRDYAESQFDRASKAKRQPGSAFKPFVYAAALEIGRTPMSVRNDVPVRIGNWTPENYDQKYRGEVTLADALANSLNTIA
AQLVMEVGPQNVVKLAHRLGIDSEMQANASIALGTSEVTLVELTSSYAPFMNGGYKATPHVIRRISTADGTVLYENTYDN
PPRVLDPAVVSEMNRMMVRVLTDGTGKNARLPGWEAAGKTGTTQSFRDALFVGYTSSLATGIWFGNDDGKFMKKVTGGGL
PARAWHDFMTAAHEGLSPSPLFGTTGVQPVFDQGIAAAEEMPVDGFDTGSPDVFPERPAGMDGMESMDGMAAADQIRPAE
QAGGPIPPAGVGETTGGTRRTTLFDLLTGG
>Mature_749_residues
ASGKSGQRIEPSFGGNDDGGELRVDASDRVAGGGRKPKRAKPPERGAKGEKRARRAGGRGSGKSGSGGFFGLARRFAYWC
VVLGIWGAIGVGGLVLYYGARMPSATSWSIPERPPNVKIVAANGEIIANRGATGGEAVALEDMSPYIPQAVIAIEDRRFH
SHFGVDPLGLARAMLTNVMSGRMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLAFWLEQKYTKDQILAMYLNRVFFGS
NAYGVEAASRRYFNKSARDVNLGEAALLAGLLKAPSRLSPARNPEAAEERAQLVLGAMREEGFINDAEIKTAMSQAPTRA
KSFWSGAEHYVADMVMDQLPGMIGEVTQDLVIDTTIDLALEKRAEEILAERLETEGGKVNASQAALVSLDGTGAIRALVG
GRDYAESQFDRASKAKRQPGSAFKPFVYAAALEIGRTPMSVRNDVPVRIGNWTPENYDQKYRGEVTLADALANSLNTIAA
QLVMEVGPQNVVKLAHRLGIDSEMQANASIALGTSEVTLVELTSSYAPFMNGGYKATPHVIRRISTADGTVLYENTYDNP
PRVLDPAVVSEMNRMMVRVLTDGTGKNARLPGWEAAGKTGTTQSFRDALFVGYTSSLATGIWFGNDDGKFMKKVTGGGLP
ARAWHDFMTAAHEGLSPSPLFGTTGVQPVFDQGIAAAEEMPVDGFDTGSPDVFPERPAGMDGMESMDGMAAADQIRPAEQ
AGGPIPPAGVGETTGGTRRTTLFDLLTGG

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=558, Percent_Identity=30.2867383512545, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788867, Length=567, Percent_Identity=31.9223985890653, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI87082258, Length=296, Percent_Identity=38.1756756756757, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789601, Length=235, Percent_Identity=34.468085106383, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80109; Mature: 79977

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01159 WW_DOMAIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGKSGQRIEPSFGGNDDGGELRVDASDRVAGGGRKPKRAKPPERGAKGEKRARRAGGR
CCCCCCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
GSGKSGSGGFFGLARRFAYWCVVLGIWGAIGVGGLVLYYGARMPSATSWSIPERPPNVKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VAANGEIIANRGATGGEAVALEDMSPYIPQAVIAIEDRRFHSHFGVDPLGLARAMLTNVM
EEECCEEEECCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHH
SGRMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLAFWLEQKYTKDQILAMYLNRVFFG
CCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SNAYGVEAASRRYFNKSARDVNLGEAALLAGLLKAPSRLSPARNPEAAEERAQLVLGAMR
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHH
EEGFINDAEIKTAMSQAPTRAKSFWSGAEHYVADMVMDQLPGMIGEVTQDLVIDTTIDLA
HCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEKRAEEILAERLETEGGKVNASQAALVSLDGTGAIRALVGGRDYAESQFDRASKAKRQP
HHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
GSAFKPFVYAAALEIGRTPMSVRNDVPVRIGNWTPENYDQKYRGEVTLADALANSLNTIA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHCCCEEHHHHHHHHHHHHH
AQLVMEVGPQNVVKLAHRLGIDSEMQANASIALGTSEVTLVELTSSYAPFMNGGYKATPH
HHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEHHHCCHHHCCCCCCCHH
VIRRISTADGTVLYENTYDNPPRVLDPAVVSEMNRMMVRVLTDGTGKNARLPGWEAAGKT
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GTTQSFRDALFVGYTSSLATGIWFGNDDGKFMKKVTGGGLPARAWHDFMTAAHEGLSPSP
CCHHHHHHHHHHEEHHHHHCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
LFGTTGVQPVFDQGIAAAEEMPVDGFDTGSPDVFPERPAGMDGMESMDGMAAADQIRPAE
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCHH
QAGGPIPPAGVGETTGGTRRTTLFDLLTGG
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ASGKSGQRIEPSFGGNDDGGELRVDASDRVAGGGRKPKRAKPPERGAKGEKRARRAGGR
CCCCCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
GSGKSGSGGFFGLARRFAYWCVVLGIWGAIGVGGLVLYYGARMPSATSWSIPERPPNVKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
VAANGEIIANRGATGGEAVALEDMSPYIPQAVIAIEDRRFHSHFGVDPLGLARAMLTNVM
EEECCEEEECCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHH
SGRMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLAFWLEQKYTKDQILAMYLNRVFFG
CCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SNAYGVEAASRRYFNKSARDVNLGEAALLAGLLKAPSRLSPARNPEAAEERAQLVLGAMR
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHH
EEGFINDAEIKTAMSQAPTRAKSFWSGAEHYVADMVMDQLPGMIGEVTQDLVIDTTIDLA
HCCCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEKRAEEILAERLETEGGKVNASQAALVSLDGTGAIRALVGGRDYAESQFDRASKAKRQP
HHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
GSAFKPFVYAAALEIGRTPMSVRNDVPVRIGNWTPENYDQKYRGEVTLADALANSLNTIA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHCCCEEHHHHHHHHHHHHH
AQLVMEVGPQNVVKLAHRLGIDSEMQANASIALGTSEVTLVELTSSYAPFMNGGYKATPH
HHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEHHHCCHHHCCCCCCCHH
VIRRISTADGTVLYENTYDNPPRVLDPAVVSEMNRMMVRVLTDGTGKNARLPGWEAAGKT
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GTTQSFRDALFVGYTSSLATGIWFGNDDGKFMKKVTGGGLPARAWHDFMTAAHEGLSPSP
CCHHHHHHHHHHEEHHHHHCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
LFGTTGVQPVFDQGIAAAEEMPVDGFDTGSPDVFPERPAGMDGMESMDGMAAADQIRPAE
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCHH
QAGGPIPPAGVGETTGGTRRTTLFDLLTGG
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]