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The map label for this gene is dapE [H]

Identifier: 150398572

GI number: 150398572

Start: 3585956

End: 3587347

Strand: Direct

Name: dapE [H]

Synonym: Smed_3383

Alternate gene names: 150398572

Gene position: 3585956-3587347 (Clockwise)

Preceding gene: 150398571

Following gene: 150398573

Centisome position: 94.82

GC content: 63.36

Gene sequence:

>1392_bases
ATGATGGCAGATATCACCCCGGTTCTCGAGCGCGCCGACGCCAATCTCCCGGCTAGCCTCAATCGGCTCTTCGACCTCGT
TCGCATCAAGTCCATTTCCACCGATCTTGCCTTCAAGGCTGAGTGCCGCAAGGCGGCCGAATGGTTGGTGGCGGAGCTCG
AAACTCTTGGATTCGAGGCCTCGCTGCGCGACACGCCTGGCCATCCGATGGTCGTAGCACACCACACAGCCGAGAAGGCG
GACGCTCCGCACCTGCTCTTCTACGGCCATTACGATGTGCAGCCGGTCGATCCCCTCAATCTCTGGGAGGCGCCGCCCTT
CGAGCCCTCCATCAAGGAGATCGAGCCCGGGCGCAAGGTCATCACCGGCCGGGGCACGGCCGACGATAAGGGCCAGTTGA
TGACTTTCGTCGAAGCGGTGCGGGCCTATAAGGAAACCCGTGCTGCGCTGCCCTGCCGCATCACCATCCTCTTCGAGGGC
GAGGAGGAATCCGGCTCTCCGTCGCTCAAGCCCTTCCTGGAAGCCAATGCCGGGGAACTCAAGGCCGACTACGCGCTCGT
CTGCGACACCAGTATGTGGGACCGGGAGACGCCCGCGATTTCGGCCGGCCTGCGCGGCCTTGTCGGCGAGGAGGTCACGA
TCAGGGCGGCGGATCGCGACCTGCACTCCGGCTATTTCGGCGGTGCGGCCGCCAACCCGATCCACATTCTGTCCGGAATT
CTTGCCGGTCTTCACGACGAGACCGGCCGCGTGACCCTCGAGGGTTTCTATGATGGCGTCGAGGAGACGCCGCCCGCGAT
CAAGGCTGCGTGGGAAACGCTCGGGCAGTCCGCCGAGAAATTTCTCGGCGAGATCGGCCTCTCGATTCCCTCGGGTGAAA
AGGGCCGCTCCGTGCTCGAACTCACCTGGGCGCGGCCGACGGCGGAGGTCAACGGTATCGCCGGGGGCTACACCGGCGAA
GGTTTCAAAACGGTGATCGCCGCCGAAGCCTCCGCGAAGGTATCGTTCCGTCTCGTCGGAAAGCAGGACCCGGACAGGAT
ACGCGAGAGCTTCCGCGCCTATGTTCGCTCGAAGATCCCGGATGACTGCTCCGTCGAGTTTCAACCGCACGGTGGTTCGC
CGGCTATCCAGCTGCCCTATGATTCGGCGCTTCTGACGACAGCGAAAACAGCACTTTCGGATGAGTGGCCGAAGCCTGCC
GTAGTCATTGGCATGGGCGGCTCCATTCCGATCGTCGGCGATTTCCAGCGCATGCTCGGCATGGAATCGCTGCTCGTCGG
CTTCGGCCTTTCCGACGACCGCATCCACTCGCCGAACGAGAAGTACGAACTGGCGTCCTTCCACAAGGGCATCCGCTCTT
GGATACGCATCCTCGACGCAATCGGCGCCTGA

Upstream 100 bases:

>100_bases
CAGGGGGAGACTTCCTGACGCGTGGCCCACCGGCAATTTAACCGGTTACGGTTTAAGCAAACATGCATTAGTTTTGCCGC
GAAACCATACAAGGATTCGC

Downstream 100 bases:

>100_bases
CGAACAGCCTTCGGCGCCGTGGGCGTTATCACAGGGATGAGAGCCCTGCAGCGTCTGCGGCGCGGAGTCGACAATTCGCG
CCGGCGGGTCGTATAACCGG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MMADITPVLERADANLPASLNRLFDLVRIKSISTDLAFKAECRKAAEWLVAELETLGFEASLRDTPGHPMVVAHHTAEKA
DAPHLLFYGHYDVQPVDPLNLWEAPPFEPSIKEIEPGRKVITGRGTADDKGQLMTFVEAVRAYKETRAALPCRITILFEG
EEESGSPSLKPFLEANAGELKADYALVCDTSMWDRETPAISAGLRGLVGEEVTIRAADRDLHSGYFGGAAANPIHILSGI
LAGLHDETGRVTLEGFYDGVEETPPAIKAAWETLGQSAEKFLGEIGLSIPSGEKGRSVLELTWARPTAEVNGIAGGYTGE
GFKTVIAAEASAKVSFRLVGKQDPDRIRESFRAYVRSKIPDDCSVEFQPHGGSPAIQLPYDSALLTTAKTALSDEWPKPA
VVIGMGGSIPIVGDFQRMLGMESLLVGFGLSDDRIHSPNEKYELASFHKGIRSWIRILDAIGA

Sequences:

>Translated_463_residues
MMADITPVLERADANLPASLNRLFDLVRIKSISTDLAFKAECRKAAEWLVAELETLGFEASLRDTPGHPMVVAHHTAEKA
DAPHLLFYGHYDVQPVDPLNLWEAPPFEPSIKEIEPGRKVITGRGTADDKGQLMTFVEAVRAYKETRAALPCRITILFEG
EEESGSPSLKPFLEANAGELKADYALVCDTSMWDRETPAISAGLRGLVGEEVTIRAADRDLHSGYFGGAAANPIHILSGI
LAGLHDETGRVTLEGFYDGVEETPPAIKAAWETLGQSAEKFLGEIGLSIPSGEKGRSVLELTWARPTAEVNGIAGGYTGE
GFKTVIAAEASAKVSFRLVGKQDPDRIRESFRAYVRSKIPDDCSVEFQPHGGSPAIQLPYDSALLTTAKTALSDEWPKPA
VVIGMGGSIPIVGDFQRMLGMESLLVGFGLSDDRIHSPNEKYELASFHKGIRSWIRILDAIGA
>Mature_463_residues
MMADITPVLERADANLPASLNRLFDLVRIKSISTDLAFKAECRKAAEWLVAELETLGFEASLRDTPGHPMVVAHHTAEKA
DAPHLLFYGHYDVQPVDPLNLWEAPPFEPSIKEIEPGRKVITGRGTADDKGQLMTFVEAVRAYKETRAALPCRITILFEG
EEESGSPSLKPFLEANAGELKADYALVCDTSMWDRETPAISAGLRGLVGEEVTIRAADRDLHSGYFGGAAANPIHILSGI
LAGLHDETGRVTLEGFYDGVEETPPAIKAAWETLGQSAEKFLGEIGLSIPSGEKGRSVLELTWARPTAEVNGIAGGYTGE
GFKTVIAAEASAKVSFRLVGKQDPDRIRESFRAYVRSKIPDDCSVEFQPHGGSPAIQLPYDSALLTTAKTALSDEWPKPA
VVIGMGGSIPIVGDFQRMLGMESLLVGFGLSDDRIHSPNEKYELASFHKGIRSWIRILDAIGA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=468, Percent_Identity=26.2820512820513, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI21071039, Length=383, Percent_Identity=30.0261096605744, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI271398277, Length=333, Percent_Identity=27.027027027027, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17563146, Length=478, Percent_Identity=29.0794979079498, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI193211059, Length=468, Percent_Identity=28.2051282051282, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=472, Percent_Identity=28.8135593220339, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6319758, Length=466, Percent_Identity=24.8927038626609, Blast_Score=116, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24585880, Length=449, Percent_Identity=30.2895322939866, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50136; Mature: 50136

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADITPVLERADANLPASLNRLFDLVRIKSISTDLAFKAECRKAAEWLVAELETLGFEA
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SLRDTPGHPMVVAHHTAEKADAPHLLFYGHYDVQPVDPLNLWEAPPFEPSIKEIEPGRKV
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCEE
ITGRGTADDKGQLMTFVEAVRAYKETRAALPCRITILFEGEEESGSPSLKPFLEANAGEL
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCC
KADYALVCDTSMWDRETPAISAGLRGLVGEEVTIRAADRDLHSGYFGGAAANPIHILSGI
CCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LAGLHDETGRVTLEGFYDGVEETPPAIKAAWETLGQSAEKFLGEIGLSIPSGEKGRSVLE
HHHCCCCCCCEEEEHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LTWARPTAEVNGIAGGYTGEGFKTVIAAEASAKVSFRLVGKQDPDRIRESFRAYVRSKIP
EEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
DDCSVEFQPHGGSPAIQLPYDSALLTTAKTALSDEWPKPAVVIGMGGSIPIVGDFQRMLG
CCCEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHHC
MESLLVGFGLSDDRIHSPNEKYELASFHKGIRSWIRILDAIGA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMADITPVLERADANLPASLNRLFDLVRIKSISTDLAFKAECRKAAEWLVAELETLGFEA
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SLRDTPGHPMVVAHHTAEKADAPHLLFYGHYDVQPVDPLNLWEAPPFEPSIKEIEPGRKV
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCEE
ITGRGTADDKGQLMTFVEAVRAYKETRAALPCRITILFEGEEESGSPSLKPFLEANAGEL
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCC
KADYALVCDTSMWDRETPAISAGLRGLVGEEVTIRAADRDLHSGYFGGAAANPIHILSGI
CCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LAGLHDETGRVTLEGFYDGVEETPPAIKAAWETLGQSAEKFLGEIGLSIPSGEKGRSVLE
HHHCCCCCCCEEEEHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LTWARPTAEVNGIAGGYTGEGFKTVIAAEASAKVSFRLVGKQDPDRIRESFRAYVRSKIP
EEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
DDCSVEFQPHGGSPAIQLPYDSALLTTAKTALSDEWPKPAVVIGMGGSIPIVGDFQRMLG
CCCEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHHC
MESLLVGFGLSDDRIHSPNEKYELASFHKGIRSWIRILDAIGA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA