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The map label for this gene is punA [H]

Identifier: 150398530

GI number: 150398530

Start: 3541280

End: 3542077

Strand: Direct

Name: punA [H]

Synonym: Smed_3341

Alternate gene names: 150398530

Gene position: 3541280-3542077 (Clockwise)

Preceding gene: 150398529

Following gene: 150398531

Centisome position: 93.64

GC content: 65.04

Gene sequence:

>798_bases
ATGACGGCGGCGGCAGAGTTCCTCAAGGGTAAACTCGGAGCGCTTGCGCCCCGCTACGCAATCGTGCTCGGTTCCGGTCT
CGGTGCGCTCGTCGACGCAGTGGCCGACCCGCTGCGTATTCCCTATGCGGAGATACCGGGCTTCCCGGTGAGTGGCGTTT
CCGGCCATGCCGGCACGTTCGTCGCGGGCAGGATCGGCGGTACTCCGGTCGCTGTGCTTTCCGGCCGGGCGCACTATTAC
GAGCGCGGCGACGCAAACGCCATGCGCGTGCCGATAGAGACGCTGAAGCGGATCGGCGTCGACAACTTGATCCTTACCAA
TTCGGCCGGGTCGCTGCGCGAAGACATGCCGCCGGGCTCCGTGATGCGGATCGCCGATCACATCGCCTTTGCCGGTGCCA
ATCCGCTGATCGGCGTGGAGAGCGACGCGCGTTTCGTGGGCATGACCAATGCATACGACGCGGCGCTGGCTGTCGGGATG
GAGGAGACCGCCGAGCGCCTCGGCATTCCGCTCGCGCGCGGCGTCTATATGTGGTTCTCCGGGCCAAGCTTCGAAACGCC
GGCCGAAATCCGCATGGCACGTATTTTAGGCGCCGATGCTGTCGGCATGTCCACGGTGCCGGAGGTCATTCTCGCCCGGT
TTTTCGGCATCAGGGTTGCCGCCGCCTCCGTCATCACCAATTTCGCTGCCGGAATGACCGGGGCCGAGCTCAGCCATGAA
GAAACCAAGGAAATGGCGCCGCTTGGCGGCACGCGCCTGGCGGCCATCCTGAAGGAAATGGTCGCGAGCGAAGGCTGA

Upstream 100 bases:

>100_bases
GCGCCAGCACCCGCATCTATCTCTGTGACGAGACGGGGATCAGGAAGACGCTGGCGCTCTCCGATCTTCTGCCGCACAGC
TTCGAGACGGAGATCCTCGG

Downstream 100 bases:

>100_bases
ATGGCGATGCTCCCGCAGGAAGTCATACGTAAGAAAAGGGATGGCGGCAGGCTCGTTCCGGCGGAGATCGCCGGTTTCAT
CGAGGGTCTGGCCGATGGCT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTAAAEFLKGKLGALAPRYAIVLGSGLGALVDAVADPLRIPYAEIPGFPVSGVSGHAGTFVAGRIGGTPVAVLSGRAHYY
ERGDANAMRVPIETLKRIGVDNLILTNSAGSLREDMPPGSVMRIADHIAFAGANPLIGVESDARFVGMTNAYDAALAVGM
EETAERLGIPLARGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVILARFFGIRVAAASVITNFAAGMTGAELSHE
ETKEMAPLGGTRLAAILKEMVASEG

Sequences:

>Translated_265_residues
MTAAAEFLKGKLGALAPRYAIVLGSGLGALVDAVADPLRIPYAEIPGFPVSGVSGHAGTFVAGRIGGTPVAVLSGRAHYY
ERGDANAMRVPIETLKRIGVDNLILTNSAGSLREDMPPGSVMRIADHIAFAGANPLIGVESDARFVGMTNAYDAALAVGM
EETAERLGIPLARGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVILARFFGIRVAAASVITNFAAGMTGAELSHE
ETKEMAPLGGTRLAAILKEMVASEG
>Mature_264_residues
TAAAEFLKGKLGALAPRYAIVLGSGLGALVDAVADPLRIPYAEIPGFPVSGVSGHAGTFVAGRIGGTPVAVLSGRAHYYE
RGDANAMRVPIETLKRIGVDNLILTNSAGSLREDMPPGSVMRIADHIAFAGANPLIGVESDARFVGMTNAYDAALAVGME
ETAERLGIPLARGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVILARFFGIRVAAASVITNFAAGMTGAELSHEE
TKEMAPLGGTRLAAILKEMVASEG

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=219, Percent_Identity=43.8356164383562, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI47132622, Length=191, Percent_Identity=30.8900523560209, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788746, Length=246, Percent_Identity=43.4959349593496, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17541190, Length=250, Percent_Identity=38.8, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323238, Length=224, Percent_Identity=43.75, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656093, Length=249, Percent_Identity=38.9558232931727, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45552885, Length=249, Percent_Identity=38.9558232931727, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24656090, Length=249, Percent_Identity=38.9558232931727, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45552887, Length=249, Percent_Identity=38.9558232931727, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=34.3096234309623, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221459247, Length=183, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 27486; Mature: 27355

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAAEFLKGKLGALAPRYAIVLGSGLGALVDAVADPLRIPYAEIPGFPVSGVSGHAGTF
CCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCE
VAGRIGGTPVAVLSGRAHYYERGDANAMRVPIETLKRIGVDNLILTNSAGSLREDMPPGS
EEECCCCCCEEEECCCHHEEECCCCCEEECCHHHHHHCCCCCEEEECCCCCHHHCCCCCH
VMRIADHIAFAGANPLIGVESDARFVGMTNAYDAALAVGMEETAERLGIPLARGVYMWFS
HHHHHHHHHHCCCCCCEECCCCCEEEECCCHHHHHHHHCHHHHHHHHCCCHHHCEEEEEC
GPSFETPAEIRMARILGADAVGMSTVPEVILARFFGIRVAAASVITNFAAGMTGAELSHE
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
ETKEMAPLGGTRLAAILKEMVASEG
HHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAAAEFLKGKLGALAPRYAIVLGSGLGALVDAVADPLRIPYAEIPGFPVSGVSGHAGTF
CHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCE
VAGRIGGTPVAVLSGRAHYYERGDANAMRVPIETLKRIGVDNLILTNSAGSLREDMPPGS
EEECCCCCCEEEECCCHHEEECCCCCEEECCHHHHHHCCCCCEEEECCCCCHHHCCCCCH
VMRIADHIAFAGANPLIGVESDARFVGMTNAYDAALAVGMEETAERLGIPLARGVYMWFS
HHHHHHHHHHCCCCCCEECCCCCEEEECCCHHHHHHHHCHHHHHHHHCCCHHHCEEEEEC
GPSFETPAEIRMARILGADAVGMSTVPEVILARFFGIRVAAASVITNFAAGMTGAELSHE
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
ETKEMAPLGGTRLAAILKEMVASEG
HHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]