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The map label for this gene is glcB

Identifier: 150398462

GI number: 150398462

Start: 3450562

End: 3452736

Strand: Direct

Name: glcB

Synonym: Smed_3270

Alternate gene names: 150398462

Gene position: 3450562-3452736 (Clockwise)

Preceding gene: 150398461

Following gene: 150398464

Centisome position: 91.24

GC content: 62.44

Gene sequence:

>2175_bases
ATGAACCGCGTTGAGCAGGACGGATTGAAGATCGATGCGGGGCTTCACCGCTTCCTGGTGGAAGAGGCGATGCCGGGCAC
CGGCGTCGATCCGGAGCGGTTCTTTTCCGCCTTCTCGGATCTCATCCACGATCTCGGACCGAAAAACCGCGCCCTTCTCG
TCAAGCGCGACGAGCTGCAGGCCAAGCTCGACGGCTGGTATCGCGATCACGGCGCGCCCGTCGACATGGACGCCTATGAA
GCCTTCCTGAAGGAAATCGGCTATCTCCTGCCGGAGGGGTCCGACTTCTCCGTCTCGACAGCCAATGTCGATCCGGAGAT
CGCCACGATCGCCGGACCGCAGCTTGTCGTGCCTGTCATGAACGCGCGCTATGCGCTCAATGCGGCCAATGCGCGCTGGG
GATCGCTCTATGACGCGCTTTACGGGACCGACGCGATTCCGGATGCGGAAGGCGCCGAGCGCGGCAAGGGCTACAATCCG
AAACGCGGGGGAAGGGTCATCGCCTGGGCACGTGATTTCCTCGACGCCAGCGCTCCGCTTTCGTCCGGCCGTTGGATCGA
TGCGACGTCTCTGGCAATCGAAGGCGCGGCACTGACCGTCACGCTTGCGAACGGCGCGAAGACCGCACTTGCCATGCCCG
CGCAATTCGCCGGCTATTCCGGAAACGCTGCAGCCCCGTCCGAGATCGTCCTTCGCAAAAACGGGCTTCACGTCGCGATC
GTCCTCGATCCGACGACTCCGATCGGCAAGGCCGATGCTGCCGGCATTGCCGATATCATTCTCGAATCGGCGATCACCAC
TATCATGGATTGCGAGGATTCGGTCGCGGCCGTCGATGCCGAGGACAAGGTGCTCGTCTATCGCAACTGGCTCGGGCTCA
TGAAGGGCGACCTCGAGGAACAGGTGGCCAAGGGCACCACCACTTTCATCCGGAGGCTCAATCCCGATCGTATCTATACT
GCATCGGGAGGCGATAAGCTGACTCTTCCGGGCCGTTCGCTGATGCTGGTGCGCAATGTCGGCCACCTGATGACCAATCC
GGCTATCCTCGACCGTGAGGGCAAGGAAGTGCCGGAGGGCCTGATGGACGCCATGGTGACGGCGCTGATCGCCTTGCACG
ACATCGGCCGGAACGGCCGCCGGGCGAATTCGCGCAGCGGCTCAATGTATGTCGTCAAGCCGAAGATGCACGGGCCCGAG
GAAGTCGCCTTCGCCTGCGAGACATTCACCCGTGTCGAGGCAGCGCTCGGGCTTCCGGCCAACGCGATGAAGATGGGAAT
AATGGACGAGGAGCGGCGGACGACCGTCAATCTCAAGGAATGCATCCGCGCGGCCCGCGAGCGCGTCGTTTTCATCAATA
CCGGCTTCCTCGACCGCACCGGCGACGAAATCCACACCTCCATGGAAGCCGGCGCGATGATCCGCAAGGGCGACATGAAG
CAGGCGGCCTGGATCTCGGCCTATGAGAACTGGAATGTCGATGTCGGTCTCGAATGCGGGCTTTCCGGTCATGCCCAGAT
CGGCAAGGGTATGTGGGCGATGCCGGACCTGATGGCGGCGATGCTGGAGCAGAAGATCGCTCATCCGAAGGCCGGTGCAA
ATACCGCCTGGGTCCCATCGCCGACGGCCGCGACGCTGCACGCAACCCATTACCACCGCATCAACGTCTCCGAGATCCAG
AATGGTCTCAGAAGCCGCTCCCGAGCGAAGCTCGCCGACATTCTTTCCGTTCCGGTGGCTGCGCGGCCGAACTGGACCGA
GGAGGAAATCCAGCGCGAACTCGACAACAATGCGCAAGGGATTCTTGGCTATGTCGTGCGCTGGGTCGACCATGGCATCG
GCTGCTCCAAGGTACCGGACATCAACAATGTCGGCCTGATGGAGGACCGTGCAACTCTGCGTATCTCTGCACAGCATATG
GCCAACTGGCTGCATCACGGCATCGTCAGCGAAGCGCAGATCGTCGAGACGATGAAGCGCATGGCCGCGGTTGTCGACGC
GCAGAACGCCGGCGACCCGAATTACCAGCCTATGGCCAGCCGCTTCGACGAATCGATCGCCTTTCAGGCGGCGCTCGATC
TGGTGCTCAAGGGTCGCGAACAGCCGAACGGCTACACCGAACCGGTTTTGCATCGCCGGCGGCTGGAGCTCAAGACGAAG
CAGCGGGCGGCGTAG

Upstream 100 bases:

>100_bases
GGAGATTTTCCGCGCGCGCGAGCGGGCGACGCGCTTTTCGAAGTGCATCCCGCTCCTATTTGTTGGACTATGGAAATAGA
GAAATTTCAGGAGTTGCCGC

Downstream 100 bases:

>100_bases
CGGGGAACCGCCGCGTCCTTGCGTCGTGCCGCTTCAAGCCAATAAGAAAGGGCCGTCGGCATTCACCGGCGGCCCTCTCC
ACTTCCGGCCCAAACGCTTA

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MNRVEQDGLKIDAGLHRFLVEEAMPGTGVDPERFFSAFSDLIHDLGPKNRALLVKRDELQAKLDGWYRDHGAPVDMDAYE
AFLKEIGYLLPEGSDFSVSTANVDPEIATIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPDAEGAERGKGYNP
KRGGRVIAWARDFLDASAPLSSGRWIDATSLAIEGAALTVTLANGAKTALAMPAQFAGYSGNAAAPSEIVLRKNGLHVAI
VLDPTTPIGKADAAGIADIILESAITTIMDCEDSVAAVDAEDKVLVYRNWLGLMKGDLEEQVAKGTTTFIRRLNPDRIYT
ASGGDKLTLPGRSLMLVRNVGHLMTNPAILDREGKEVPEGLMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPE
EVAFACETFTRVEAALGLPANAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGAMIRKGDMK
QAAWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHRINVSEIQ
NGLRSRSRAKLADILSVPVAARPNWTEEEIQRELDNNAQGILGYVVRWVDHGIGCSKVPDINNVGLMEDRATLRISAQHM
ANWLHHGIVSEAQIVETMKRMAAVVDAQNAGDPNYQPMASRFDESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKTK
QRAA

Sequences:

>Translated_724_residues
MNRVEQDGLKIDAGLHRFLVEEAMPGTGVDPERFFSAFSDLIHDLGPKNRALLVKRDELQAKLDGWYRDHGAPVDMDAYE
AFLKEIGYLLPEGSDFSVSTANVDPEIATIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPDAEGAERGKGYNP
KRGGRVIAWARDFLDASAPLSSGRWIDATSLAIEGAALTVTLANGAKTALAMPAQFAGYSGNAAAPSEIVLRKNGLHVAI
VLDPTTPIGKADAAGIADIILESAITTIMDCEDSVAAVDAEDKVLVYRNWLGLMKGDLEEQVAKGTTTFIRRLNPDRIYT
ASGGDKLTLPGRSLMLVRNVGHLMTNPAILDREGKEVPEGLMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPE
EVAFACETFTRVEAALGLPANAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGAMIRKGDMK
QAAWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHRINVSEIQ
NGLRSRSRAKLADILSVPVAARPNWTEEEIQRELDNNAQGILGYVVRWVDHGIGCSKVPDINNVGLMEDRATLRISAQHM
ANWLHHGIVSEAQIVETMKRMAAVVDAQNAGDPNYQPMASRFDESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKTK
QRAA
>Mature_724_residues
MNRVEQDGLKIDAGLHRFLVEEAMPGTGVDPERFFSAFSDLIHDLGPKNRALLVKRDELQAKLDGWYRDHGAPVDMDAYE
AFLKEIGYLLPEGSDFSVSTANVDPEIATIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPDAEGAERGKGYNP
KRGGRVIAWARDFLDASAPLSSGRWIDATSLAIEGAALTVTLANGAKTALAMPAQFAGYSGNAAAPSEIVLRKNGLHVAI
VLDPTTPIGKADAAGIADIILESAITTIMDCEDSVAAVDAEDKVLVYRNWLGLMKGDLEEQVAKGTTTFIRRLNPDRIYT
ASGGDKLTLPGRSLMLVRNVGHLMTNPAILDREGKEVPEGLMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPE
EVAFACETFTRVEAALGLPANAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGAMIRKGDMK
QAAWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHRINVSEIQ
NGLRSRSRAKLADILSVPVAARPNWTEEEIQRELDNNAQGILGYVVRWVDHGIGCSKVPDINNVGLMEDRATLRISAQHM
ANWLHHGIVSEAQIVETMKRMAAVVDAQNAGDPNYQPMASRFDESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKTK
QRAA

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=721, Percent_Identity=59.0846047156727, Blast_Score=878, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_SINMW (A6UEL4)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328929.1
- ProteinModelPortal:   A6UEL4
- SMR:   A6UEL4
- STRING:   A6UEL4
- GeneID:   5324149
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3270
- eggNOG:   COG2225
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- BioCyc:   SMED366394:SMED_3270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 78837; Mature: 78837

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 629-629

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVEQDGLKIDAGLHRFLVEEAMPGTGVDPERFFSAFSDLIHDLGPKNRALLVKRDELQ
CCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
AKLDGWYRDHGAPVDMDAYEAFLKEIGYLLPEGSDFSVSTANVDPEIATIAGPQLVVPVM
HHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCEEEEEEC
NARYALNAANARWGSLYDALYGTDAIPDAEGAERGKGYNPKRGGRVIAWARDFLDASAPL
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEEHHHHCCCCCC
SSGRWIDATSLAIEGAALTVTLANGAKTALAMPAQFAGYSGNAAAPSEIVLRKNGLHVAI
CCCCEEEHHHEEECCEEEEEEECCCCCHHHCCCHHHCCCCCCCCCCCCEEEEECCCEEEE
VLDPTTPIGKADAAGIADIILESAITTIMDCEDSVAAVDAEDKVLVYRNWLGLMKGDLEE
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEHHHHHHHCCHHH
QVAKGTTTFIRRLNPDRIYTASGGDKLTLPGRSLMLVRNVGHLMTNPAILDREGKEVPEG
HHHCCHHHHHHHCCCCEEEECCCCCEEECCCCCEEEHHHHHHHHCCCCEECCCCCHHHHH
LMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPEEVAFACETFTRVEAALGLPA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
NAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGAMIRKGDMK
HHHHCCCCCCHHHCEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCEECCCCH
QAAWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHRINVSEIQNGLRSRSRAKLADILSVPVAARPNWTEEEIQRELDNNAQG
CCCEEEEECEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHH
ILGYVVRWVDHGIGCSKVPDINNVGLMEDRATLRISAQHMANWLHHGIVSEAQIVETMKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHH
MAAVVDAQNAGDPNYQPMASRFDESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKTK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRAA
HCCC
>Mature Secondary Structure
MNRVEQDGLKIDAGLHRFLVEEAMPGTGVDPERFFSAFSDLIHDLGPKNRALLVKRDELQ
CCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
AKLDGWYRDHGAPVDMDAYEAFLKEIGYLLPEGSDFSVSTANVDPEIATIAGPQLVVPVM
HHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCEEEEEEC
NARYALNAANARWGSLYDALYGTDAIPDAEGAERGKGYNPKRGGRVIAWARDFLDASAPL
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEEHHHHCCCCCC
SSGRWIDATSLAIEGAALTVTLANGAKTALAMPAQFAGYSGNAAAPSEIVLRKNGLHVAI
CCCCEEEHHHEEECCEEEEEEECCCCCHHHCCCHHHCCCCCCCCCCCCEEEEECCCEEEE
VLDPTTPIGKADAAGIADIILESAITTIMDCEDSVAAVDAEDKVLVYRNWLGLMKGDLEE
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEHHHHHHHCCHHH
QVAKGTTTFIRRLNPDRIYTASGGDKLTLPGRSLMLVRNVGHLMTNPAILDREGKEVPEG
HHHCCHHHHHHHCCCCEEEECCCCCEEECCCCCEEEHHHHHHHHCCCCEECCCCCHHHHH
LMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPEEVAFACETFTRVEAALGLPA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
NAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGAMIRKGDMK
HHHHCCCCCCHHHCEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCEECCCCH
QAAWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHRINVSEIQNGLRSRSRAKLADILSVPVAARPNWTEEEIQRELDNNAQG
CCCEEEEECEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHH
ILGYVVRWVDHGIGCSKVPDINNVGLMEDRATLRISAQHMANWLHHGIVSEAQIVETMKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHH
MAAVVDAQNAGDPNYQPMASRFDESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKTK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA