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The map label for this gene is trpF

Identifier: 150398428

GI number: 150398428

Start: 3411141

End: 3411788

Strand: Direct

Name: trpF

Synonym: Smed_3236

Alternate gene names: 150398428

Gene position: 3411141-3411788 (Clockwise)

Preceding gene: 150398427

Following gene: 150398429

Centisome position: 90.2

GC content: 60.03

Gene sequence:

>648_bases
ATGAAAACGGAAGTGAAGATTTGCGGCTTGAAGACGGCCGAGGCCCTGCAACGTGCCGTTGCGCTCGGTGCTTCCCACAC
GGGTTTTATCTTCTTTCCCAAGAGCCCACGCAATATCGAGCCGGATGACGCCGGCCGTCTTGCCGAGCTCGCCCGAGGAA
GGGCGAAGATCGTCGCGGTCACTGTCGATGCGGATAATGACGATCTCGACGAAATCGTTTCGGCGCTTCATCCCGAGGTG
CTGCAGCTTCATGGTTCTGAGAATCCGGAGCGCGTGCTTGCGATCAAGGCTCTTTACGGCCTTCCCGTCATCAAGGCGCT
CCCGATCCGCGAGGCTTCCGACCTGGAGCGGATCGCGCCCTATATCGGGATCGCCGATCGTTTTCTCCTGGATGCGAAGC
CGCCGGCTGGTTCCGACCTGCCGGGTGGAAACGGGGTTTCTTTCGACTGGAGACTCCTCGACGCGCTTGACGCGAACGTC
GATTACATGCTTTCCGGTGGATTGAATGCGAGCAATGTCGAAGACGCGCTTGCGCTGACCGGCGCGCGCGCCATCGATAC
CTCCTCCGGGGTCGAAAGCGCACCGGGCATCAAGGATCTGACGCTCATGGATGCGTTTTTCGAGGCGATTCGCCGAGCGG
AAGCTTAA

Upstream 100 bases:

>100_bases
AAAACGCCGGGTGCCCGCCGCCGCATTCTTCCTAACAATAGCCACGGCAACGCAACGGTTTTGCTCGACTCTGGCGGCAT
TTCATGCAAGGTCGCTTTCC

Downstream 100 bases:

>100_bases
GCCACGATCAGGGAGCAAGACGTGAATCAGCCGCCTAAACCGAATTCCTTCAGATCCGGACCCGATGAAGAGGGCCGTTT
CGGCATATTCGGTGGCCGCT

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKTEVKICGLKTAEALQRAVALGASHTGFIFFPKSPRNIEPDDAGRLAELARGRAKIVAVTVDADNDDLDEIVSALHPEV
LQLHGSENPERVLAIKALYGLPVIKALPIREASDLERIAPYIGIADRFLLDAKPPAGSDLPGGNGVSFDWRLLDALDANV
DYMLSGGLNASNVEDALALTGARAIDTSSGVESAPGIKDLTLMDAFFEAIRRAEA

Sequences:

>Translated_215_residues
MKTEVKICGLKTAEALQRAVALGASHTGFIFFPKSPRNIEPDDAGRLAELARGRAKIVAVTVDADNDDLDEIVSALHPEV
LQLHGSENPERVLAIKALYGLPVIKALPIREASDLERIAPYIGIADRFLLDAKPPAGSDLPGGNGVSFDWRLLDALDANV
DYMLSGGLNASNVEDALALTGARAIDTSSGVESAPGIKDLTLMDAFFEAIRRAEA
>Mature_215_residues
MKTEVKICGLKTAEALQRAVALGASHTGFIFFPKSPRNIEPDDAGRLAELARGRAKIVAVTVDADNDDLDEIVSALHPEV
LQLHGSENPERVLAIKALYGLPVIKALPIREASDLERIAPYIGIADRFLLDAKPPAGSDLPGGNGVSFDWRLLDALDANV
DYMLSGGLNASNVEDALALTGARAIDTSSGVESAPGIKDLTLMDAFFEAIRRAEA

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=219, Percent_Identity=31.9634703196347, Blast_Score=84, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320210, Length=219, Percent_Identity=29.2237442922374, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_SINMW (A6UEI0)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328895.1
- STRING:   A6UEI0
- GeneID:   5324115
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3236
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- BioCyc:   SMED366394:SMED_3236-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 22843; Mature: 22843

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEVKICGLKTAEALQRAVALGASHTGFIFFPKSPRNIEPDDAGRLAELARGRAKIVAV
CCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEE
TVDADNDDLDEIVSALHPEVLQLHGSENPERVLAIKALYGLPVIKALPIREASDLERIAP
EEECCCCCHHHHHHHHCHHHEECCCCCCHHHEEEHHHHHCCCHHEECCCCCHHHHHHHHH
YIGIADRFLLDAKPPAGSDLPGGNGVSFDWRLLDALDANVDYMLSGGLNASNVEDALALT
HHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHC
GARAIDTSSGVESAPGIKDLTLMDAFFEAIRRAEA
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTEVKICGLKTAEALQRAVALGASHTGFIFFPKSPRNIEPDDAGRLAELARGRAKIVAV
CCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEE
TVDADNDDLDEIVSALHPEVLQLHGSENPERVLAIKALYGLPVIKALPIREASDLERIAP
EEECCCCCHHHHHHHHCHHHEECCCCCCHHHEEEHHHHHCCCHHEECCCCCHHHHHHHHH
YIGIADRFLLDAKPPAGSDLPGGNGVSFDWRLLDALDANVDYMLSGGLNASNVEDALALT
HHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHC
GARAIDTSSGVESAPGIKDLTLMDAFFEAIRRAEA
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA