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The map label for this gene is rpoH2 [H]

Identifier: 150398305

GI number: 150398305

Start: 3258437

End: 3259303

Strand: Reverse

Name: rpoH2 [H]

Synonym: Smed_3111

Alternate gene names: 150398305

Gene position: 3259303-3258437 (Counterclockwise)

Preceding gene: 150398306

Following gene: 150398300

Centisome position: 86.18

GC content: 63.78

Gene sequence:

>867_bases
ATGAAGACCCTCACAGCAGACAGGCGTATGATCAAGATTGCCATGGAAGCGCCCTATCTCGAGCGGGACGAGGAACATGC
GCTCGCGGAGGCATGGCGCAACGACAACGACCAGGAGGCGCGCAACAAGATCGCGATGTCGCATATGCGGCTGGTCATCT
CGATGGCGGCGAAGTTCCGTAGCTTTGGGCTGCCCATGGGAGATCTCGTGCAGGAGGGGCACATCGGTCTATTGGAAGCC
GCAGCGCGCTTCGAACCGAGCCGTGAGGTGCGTTTTTCGACCTATGCGACCTGGTGGATCCGAGCCTCGATGCAGGACTA
TGTGCTGCGCAACTGGTCGATCGTGCGCGGGGGAACCAGTTCGGCGCAGAAGGCGCTTTTCTTCAACCTGCGCCGGCTGC
GGGCCCGGCTCGCACAGGGCGACAGGCAGTTGACCTCGCAGGCGGTGCATGAGGAGATCGCCGCCGCAATCGGCGTGAGT
CTCGCCGACGTACAAACGATGGATGCACGCCTCTCTGGAAACGACGCGTCGCTGCAGGCCCCCATAGGCGGCGGCGACCC
GGATGCGGGGGCCCGGCTCGATTTTCTGGCGAGCGAGGCGCCTCTGCCCGATGAGCAGGTGAGCGACCTGATCGACGGGG
AGCGCGCCCGACGGTGGCTGCACGTCGCTCTCGGGGAGCTTAGCGAACGCGAAATGAAGATCATCCGCGCACGGCGTCTC
TCCGAGGACGGTGCAACATTGGAGGAACTCGGTGTTGCGCTTGGAATATCGAAGGAGCGCGTGCGGCAAATTGAAACACG
TGCACTTGAAAAGCTGCGCGCGGCACTCACCGCCAGGGCCCCGGCCCTGACGGCGGCCCTGCATTAA

Upstream 100 bases:

>100_bases
AACCAGCGCCATCCGTCCCGTTGCCGAACGCGGCAAGAGACCGCTCGGCCTTTGGAGTTCAATCGCGAAATGGCGCTCGA
CGCCAGCTACGGAGCAATCG

Downstream 100 bases:

>100_bases
GTCAAAGCCTGGCGAGATCGTTTGCTCCTCATCCGCCTACCGGTAATTTCTCGCCGCAAGCGGTGCGAAGGGACCAGCAA
AGCTCGCTTTGCCTTGCACA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKTLTADRRMIKIAMEAPYLERDEEHALAEAWRNDNDQEARNKIAMSHMRLVISMAAKFRSFGLPMGDLVQEGHIGLLEA
AARFEPSREVRFSTYATWWIRASMQDYVLRNWSIVRGGTSSAQKALFFNLRRLRARLAQGDRQLTSQAVHEEIAAAIGVS
LADVQTMDARLSGNDASLQAPIGGGDPDAGARLDFLASEAPLPDEQVSDLIDGERARRWLHVALGELSEREMKIIRARRL
SEDGATLEELGVALGISKERVRQIETRALEKLRAALTARAPALTAALH

Sequences:

>Translated_288_residues
MKTLTADRRMIKIAMEAPYLERDEEHALAEAWRNDNDQEARNKIAMSHMRLVISMAAKFRSFGLPMGDLVQEGHIGLLEA
AARFEPSREVRFSTYATWWIRASMQDYVLRNWSIVRGGTSSAQKALFFNLRRLRARLAQGDRQLTSQAVHEEIAAAIGVS
LADVQTMDARLSGNDASLQAPIGGGDPDAGARLDFLASEAPLPDEQVSDLIDGERARRWLHVALGELSEREMKIIRARRL
SEDGATLEELGVALGISKERVRQIETRALEKLRAALTARAPALTAALH
>Mature_288_residues
MKTLTADRRMIKIAMEAPYLERDEEHALAEAWRNDNDQEARNKIAMSHMRLVISMAAKFRSFGLPMGDLVQEGHIGLLEA
AARFEPSREVRFSTYATWWIRASMQDYVLRNWSIVRGGTSSAQKALFFNLRRLRARLAQGDRQLTSQAVHEEIAAAIGVS
LADVQTMDARLSGNDASLQAPIGGGDPDAGARLDFLASEAPLPDEQVSDLIDGERARRWLHVALGELSEREMKIIRARRL
SEDGATLEELGVALGISKERVRQIETRALEKLRAALTARAPALTAALH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=39.6296296296296, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789098, Length=253, Percent_Identity=32.8063241106719, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=31.9327731092437, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32009; Mature: 32009

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTADRRMIKIAMEAPYLERDEEHALAEAWRNDNDQEARNKIAMSHMRLVISMAAKFR
CCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFGLPMGDLVQEGHIGLLEAAARFEPSREVRFSTYATWWIRASMQDYVLRNWSIVRGGTS
HHCCCHHHHHHCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRARLAQGDRQLTSQAVHEEIAAAIGVSLADVQTMDARLSGNDASLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCEEEC
PIGGGDPDAGARLDFLASEAPLPDEQVSDLIDGERARRWLHVALGELSEREMKIIRARRL
CCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SEDGATLEELGVALGISKERVRQIETRALEKLRAALTARAPALTAALH
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MKTLTADRRMIKIAMEAPYLERDEEHALAEAWRNDNDQEARNKIAMSHMRLVISMAAKFR
CCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFGLPMGDLVQEGHIGLLEAAARFEPSREVRFSTYATWWIRASMQDYVLRNWSIVRGGTS
HHCCCHHHHHHCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRARLAQGDRQLTSQAVHEEIAAAIGVSLADVQTMDARLSGNDASLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCEEEC
PIGGGDPDAGARLDFLASEAPLPDEQVSDLIDGERARRWLHVALGELSEREMKIIRARRL
CCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SEDGATLEELGVALGISKERVRQIETRALEKLRAALTARAPALTAALH
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]