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The map label for this gene is tuf2 [H]

Identifier: 150396190

GI number: 150396190

Start: 1046647

End: 1047822

Strand: Direct

Name: tuf2 [H]

Synonym: Smed_0969

Alternate gene names: 150396190

Gene position: 1046647-1047822 (Clockwise)

Preceding gene: 150396188

Following gene: 150396191

Centisome position: 27.68

GC content: 61.31

Gene sequence:

>1176_bases
ATGGCAAAGAGCAAATTTGAGCGCAATAAGCCGCACGTCAACATTGGCACGATTGGCCACGTTGACCATGGCAAGACGTC
GCTGACGGCAGCGATCACGAAGTATTTCGGCGAGTTCAAGGCGTACGACCAGATCGACGCTGCGCCGGAAGAAAAGGCCC
GCGGTATCACCATTTCGACGGCGCACGTCGAATACGAGACGCCGAACCGTCACTATGCGCATGTCGACTGCCCCGGCCAC
GCCGACTACGTCAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTGGTTGTTTCGGCTGCCGACGGCCC
GATGCCGCAGACGCGCGAACACATTCTGCTGGCCCGCCAGGTCGGCGTTCCGGCGATTGTCGTGTTCCTGAACAAGGTCG
ACCAGGTCGACGATGCGGAGCTGCTGGAGCTCGTTGAGCTGGAAGTGCGCGAACTTCTGTCGTCCTACGAATTCCCGGGC
GACGATATTCCGATCGTCAAGGGTTCGGCTCTGGCCGCACTTGAAGATTCGGACAAGAAGATCGGCGAAGATGCGATCCG
CGAGCTGATGGCTGCGGTTGACGCCTACATCCCGACGCCTGAGCGTCCGATCGACCAGCCGTTCCTGATGCCGATCGAAG
ACGTGTTCTCGATCTCGGGCCGCGGCACGGTCGTGACCGGCCGCGTCGAGCGCGGCATCGTCAAGGTCGGTGAGGAAATC
GAAATCGTCGGCATCCGTCCGACGACGAAGACGACCTGCACGGGCGTTGAAATGTTCCGCAAGCTTCTCGATCAGGGCCA
GGCCGGCGACAACATCGGCGCGCTTCTGCGCGGTGTCGACCGTAACGGTGTCGAGCGCGGCCAGATTCTGTGCAAGCCGG
GTTCGGTGAAGCCGCACCGCAAGTTCAAGGCTGAAGCCTACATCCTGACGAAGGAAGAGGGTGGCCGTCATACGCCGTTC
TTCACCAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCATCGTGACGCTGCCGGAAGGCACGGAAAT
GGTTATGCCGGGCGACAACGTGACCGTTGACGTCGAGCTGATCGTGCCGATCGCGATGGAAGAAAAGCTGCGCTTCGCTA
TCCGCGAAGGCGGCCGTACCGTCGGCGCAGGCATCGTCGCCTCCATCGTCGAGTAA

Upstream 100 bases:

>100_bases
ACGCAATTAAGTCTTGCGCAAATGCGACGAAACCCTTAAACGGCTCGACAAACCGGCCCCGCCGGCTGATCCATCCGATT
TGACCACAGGAAGCGTACCC

Downstream 100 bases:

>100_bases
ATTCCGGTCCAACCGGACGTTGAAAACCCCGCGGGCAACCGCGGGGTTTTTGCTTTCAAGGCCGGAGCGGGATGAGGAAA
TGTGTTCGCGGCATTCGGCC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPNRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYEFPG
DDIPIVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEI
EIVGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDRNGVERGQILCKPGSVKPHRKFKAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVDVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Sequences:

>Translated_391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPNRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYEFPG
DDIPIVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEI
EIVGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDRNGVERGQILCKPGSVKPHRKFKAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVDVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE
>Mature_390_residues
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPNRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYEFPGD
DIPIVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEIE
IVGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDRNGVERGQILCKPGSVKPHRKFKAEAYILTKEEGGRHTPFF
TNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVDVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=55.0761421319797, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=29.8165137614679, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.8165137614679, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=28.9592760180996, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=27.6785714285714, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=26.517571884984, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=29.1545189504373, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194097354, Length=347, Percent_Identity=29.971181556196, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018520, Length=347, Percent_Identity=29.971181556196, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI46094014, Length=327, Percent_Identity=29.9694189602446, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=27.5092936802974, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=31.25, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.9367088607595, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.9367088607595, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=31.3829787234043, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI48994988, Length=249, Percent_Identity=32.5301204819277, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789108, Length=267, Percent_Identity=28.4644194756554, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.5, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=30.4054054054054, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=30.4054054054054, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=28.1395348837209, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=26.2443438914027, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=441, Percent_Identity=26.530612244898, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=442, Percent_Identity=26.2443438914027, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=295, Percent_Identity=29.8305084745763, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=34.3373493975904, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=26.7148014440433, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.7056277056277, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.3266331658292, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=29.7968397291196, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=29.7968397291196, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=274, Percent_Identity=27.7372262773723, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.6135693215339, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=28.2527881040892, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=30.1675977653631, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=31.2977099236641, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.4590163934426, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.0152671755725, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.0152671755725, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=50, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=28.8888888888889, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=28.8888888888889, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=28.8888888888889, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=28.8888888888889, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=29.3333333333333, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=29.3333333333333, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=29.2899408284024, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31.6666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.673590504451, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24647046, Length=276, Percent_Identity=26.8115942028986, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647048, Length=276, Percent_Identity=26.8115942028986, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=159, Percent_Identity=36.4779874213836, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=31.7567567567568, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42749; Mature: 42618

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCEEEEE
AHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYEFPGDDIPIVKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHCCCCEE
EIVGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDRNGVERGQILCKPGSVKPHR
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCC
KFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVDVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCEEEEE
AHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYEFPGDDIPIVKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHCCCCEE
EIVGIRPTTKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDRNGVERGQILCKPGSVKPHR
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCC
KFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVDVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EEEEHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA