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The map label for this gene is prfB [H]

Identifier: 150396182

GI number: 150396182

Start: 1039191

End: 1040219

Strand: Direct

Name: prfB [H]

Synonym: Smed_0961

Alternate gene names: 150396182

Gene position: 1039191-1040219 (Clockwise)

Preceding gene: 150396181

Following gene: 150396185

Centisome position: 27.48

GC content: 61.9

Gene sequence:

>1029_bases
TTGAACAACAAGGCCGAAGACCCGTCGCTCTGGAACGACGCGGCAGAGGCACAGAAACTGATGCGGGAGCGCCAGCAGCT
CGACGATGGCATCAGTGGCCTGCGTCGGTTCGAGCAGCAGCTCAACGACAACATCGAGCTCATCGAACTCGGCGAAGAGG
AAGGCGATTCGGCAATCGTCTCGGAGGCCGAGGAAGCGCTCCGCCAACTGCGCAACGACGCAGCTAAAAAGCAGGTGGAG
GCCATGCTCTCCGGGGAGGCCGACCAGAACGATACCTATGTCGAAGTCCATTCGGGCGCCGGTGGCACGGAAAGCCAGGA
CTGGGCCAACATGCTGCTTCGCATGTATACGCGATGGGCCGAGCGCCAGGGCTACAAGGTCGAGCTCATGGAAATCCAGG
ATGGGGAAGAGGCCGGCATCAAGTCCGCAACGCTCCTGGTCAAGGGCCACAATGCCTATGGCTGGCTGAAGACGGAATCG
GGCGTGCACCGGCTGGTCCGCATTTCCCCCTATGACAGCAATGCGCGCCGTCATACGTCCTTCTCGTCCATCTGGGTCTA
TCCCGTGGTGGACGAATCGATCCAGGTCGACATCAATGAAAGCGATTGCAGGATCGATACCTACCGCTCCTCCGGCGCCG
GCGGTCAGCACGTCAACACGACCGATTCGGCCGTGCGCATCACGCATATGCCGAGCGGCATCGTGGTGCAGTGCCAGCAG
GAACGCTCACAGCATAAGAACCGCGCCAAGGCATGGGACATGCTCCGTGCGCGGCTTTACGAGGCCGAACTGAAGAAGCG
CGAGGAGGCGGCCAACGCCGAGGCGGCATCGAAGACCGAGATCGGCTGGGGTCACCAGATCCGCTCCTACGTCCTGCAGC
CCTACCAGCTCGTGAAGGACTTGAGGACAGGCGTCGAAAGCACAAGCCCGGGCGACGTGCTGGACGGTGAACTGAGCGAG
TTCATGGAGGCAGCCCTTGCGCATCGCGTAAGCGGTGGGGCGGATGCGGCGGTCGACGATTTGAGTTGA

Upstream 100 bases:

>100_bases
CGCAGCGAAATCGAGAACATCGTCGACGAAATCAAGCAGGCCATAAGCCTGCTGAGGAGGCATCTTTGACTGGGATCAGG
CGGTAAGACGACTGGACTGG

Downstream 100 bases:

>100_bases
TGTTTTTATCCGACGCGCAAACGCGGCGCCGTGCGCCGCGTTTGCGTTGGAAATGACACGAGTGTGGGCGTGAAGGACGA
GGCGGGGGCGCTCTACAAGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNNKAEDPSLWNDAAEAQKLMRERQQLDDGISGLRRFEQQLNDNIELIELGEEEGDSAIVSEAEEALRQLRNDAAKKQVE
AMLSGEADQNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGYKVELMEIQDGEEAGIKSATLLVKGHNAYGWLKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDESIQVDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHMPSGIVVQCQQ
ERSQHKNRAKAWDMLRARLYEAELKKREEAANAEAASKTEIGWGHQIRSYVLQPYQLVKDLRTGVESTSPGDVLDGELSE
FMEAALAHRVSGGADAAVDDLS

Sequences:

>Translated_342_residues
MNNKAEDPSLWNDAAEAQKLMRERQQLDDGISGLRRFEQQLNDNIELIELGEEEGDSAIVSEAEEALRQLRNDAAKKQVE
AMLSGEADQNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGYKVELMEIQDGEEAGIKSATLLVKGHNAYGWLKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDESIQVDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHMPSGIVVQCQQ
ERSQHKNRAKAWDMLRARLYEAELKKREEAANAEAASKTEIGWGHQIRSYVLQPYQLVKDLRTGVESTSPGDVLDGELSE
FMEAALAHRVSGGADAAVDDLS
>Mature_342_residues
MNNKAEDPSLWNDAAEAQKLMRERQQLDDGISGLRRFEQQLNDNIELIELGEEEGDSAIVSEAEEALRQLRNDAAKKQVE
AMLSGEADQNDTYVEVHSGAGGTESQDWANMLLRMYTRWAERQGYKVELMEIQDGEEAGIKSATLLVKGHNAYGWLKTES
GVHRLVRISPYDSNARRHTSFSSIWVYPVVDESIQVDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHMPSGIVVQCQQ
ERSQHKNRAKAWDMLRARLYEAELKKREEAANAEAASKTEIGWGHQIRSYVLQPYQLVKDLRTGVESTSPGDVLDGELSE
FMEAALAHRVSGGADAAVDDLS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=38.562091503268, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=32.398753894081, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=37.914691943128, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=52.1472392638037, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=44.0329218106996, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=247, Percent_Identity=34.0080971659919, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=241, Percent_Identity=40.6639004149378, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=43.3673469387755, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38184; Mature: 38184

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKAEDPSLWNDAAEAQKLMRERQQLDDGISGLRRFEQQLNDNIELIELGEEEGDSAIV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
SEAEEALRQLRNDAAKKQVEAMLSGEADQNDTYVEVHSGAGGTESQDWANMLLRMYTRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
ERQGYKVELMEIQDGEEAGIKSATLLVKGHNAYGWLKTESGVHRLVRISPYDSNARRHTS
HCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCC
FSSIWVYPVVDESIQVDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHMPSGIVVQCQQ
CCEEEEEEEECCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHH
ERSQHKNRAKAWDMLRARLYEAELKKREEAANAEAASKTEIGWGHQIRSYVLQPYQLVKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRTGVESTSPGDVLDGELSEFMEAALAHRVSGGADAAVDDLS
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNNKAEDPSLWNDAAEAQKLMRERQQLDDGISGLRRFEQQLNDNIELIELGEEEGDSAIV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
SEAEEALRQLRNDAAKKQVEAMLSGEADQNDTYVEVHSGAGGTESQDWANMLLRMYTRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
ERQGYKVELMEIQDGEEAGIKSATLLVKGHNAYGWLKTESGVHRLVRISPYDSNARRHTS
HCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCC
FSSIWVYPVVDESIQVDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHMPSGIVVQCQQ
CCEEEEEEEECCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHH
ERSQHKNRAKAWDMLRARLYEAELKKREEAANAEAASKTEIGWGHQIRSYVLQPYQLVKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRTGVESTSPGDVLDGELSEFMEAALAHRVSGGADAAVDDLS
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA