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The map label for this gene is gatB [H]

Identifier: 150396169

GI number: 150396169

Start: 1022099

End: 1023601

Strand: Direct

Name: gatB [H]

Synonym: Smed_0946

Alternate gene names: 150396169

Gene position: 1022099-1023601 (Clockwise)

Preceding gene: 150396167

Following gene: 150396170

Centisome position: 27.03

GC content: 60.48

Gene sequence:

>1503_bases
ATGAGCATCGTCGACGTCCGCACTCCTGATCCGAAACGCTTCATTCCGGGCGCCACCGGCGACTGGGAGGTCATCATCGG
CATGGAAGTCCATGCGCAGGTGCTGAGCAATTCGAAGCTGTTCTCCGGTGCATCGACCGAGTTCGGGTCCGAGCCGAACG
CCAACGTTTCGCTTGTCGATGCTGCAATGCCCGGCATGCTTCCGGTGATCAATGAGGAATGCGTCAAGCAGGCCGTGCGC
ACGGGCCTCGGCTTGAAGGCAAAGATCAACAATCGTTCGATCTTCGATCGGAAGAACTACTTCTATCCGGACCTGCCACA
GGGCTACCAGATCTCGCAATACAAGGACCCGATTGTCGGAGAAGGCAAGATCATCATCTCCGTCGGACCGGACCGCCAGG
GCCATTTCGAAGATGTGGAGATCGGCATCGAGCGTCTGCATCTGGAGCAGGATGCCGGCAAGTCGATGCACGACCAGCAT
CCAGCCATGTCCTATGTCGATCTCAACCGCTCGGGTGTTGCGCTGATGGAGATCGTCTCGAAGCCGGATCTGCGTTCGTC
GGACGAGGCGAAGGCCTATCTCACGAAGCTTCGCTCGATCCTGCGCTATCTCGGCACCTGCGACGGCAATATGGACGAGG
GCTCGATGCGCGCGGACGTCAACGTCTCCGTTCGCCGCCCCGGCGAAGCATTCGGCACGCGCTGTGAAATCAAGAACGTC
AACTCGATCCGCTTCGTCGGTCAGGCGATCGAATACGAGGCCCGTCGCCAGATCGCCATCCTTGAAGACGGTGGGGCAAT
CGATCAGGAAACGCGTCTCTTCGACCCGGGCAAGGGCGAGACGCGTTCCATGCGGTCGAAGGAAGATGCGCACGACTACC
GCTACTTCCCCGACCCGGATCTGCTGCCGCTCGAATTCGACGATGCGTTCGTAGAGGCGCTGAAGGCACACCTGCCGGAG
TTGCCGGACGACAAGAAGGAGCGCTTCGTCAGAGATCTCGGCCTGTCCGTCTACGACGCGTCCGTTCTCGTGTCGGAAAA
GGCGATCGCCGACTACTTCGAGGCCGTGGCAGCTGGTCGCGACGGCAAGACCGCGGCGAACTGGGTCATCAACGACCTTC
TCGGCGCGCTGAACAAGGCTGGAAAGCCTATTGAGGGAACGCCCGTCTCGCCGGCACAGCTTGGTGGCATCATCGATCTC
ATCAAGGATGGGACCATCTCCGGCAAGCTTGCGAAGGACCTCTTCGAAATAGTCTGGAACGAGGGCGGCGATCCGGCGCA
GATTGTCGAGAGCCGTGGTATGAAGCAGGTAACCGATACCGGTGCGATCGAGAAGGCCGTCGACGAGATCATCGCAGCCA
ACCCCGACCAGGTGGAAAAGGCGAAGGCGAAGCCCTCGCTTGCCGGCTGGTTTGTAGGGCAGGTGATGAAGGCGACCGGC
GGCAAGGCCAACCCGCAGGCGGTGCAGGCGCTCGTAAAGTCCAAGCTCGGATTCGAGGAATGA

Upstream 100 bases:

>100_bases
GCCCGGCACCCGATACGCGGCCGCCCCTTACAAGCTTGCACCCAGTGGCCAATTGCTCTACCGAGAAACCACGAATTCAG
GCCTCAAAGAAGAGCATGAC

Downstream 100 bases:

>100_bases
CGGTGTTCTTCGTCCGTACCGCAGGCGAGCGCGACCTCGATAAGGTTCGCGCTCTGCTCGCCGAGACGTGGCATGCAACC
TACGACACGTTCTACGGTGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSIVDVRTPDPKRFIPGATGDWEVIIGMEVHAQVLSNSKLFSGASTEFGSEPNANVSLVDAAMPGMLPVINEECVKQAVR
TGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQYKDPIVGEGKIIISVGPDRQGHFEDVEIGIERLHLEQDAGKSMHDQH
PAMSYVDLNRSGVALMEIVSKPDLRSSDEAKAYLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEAFGTRCEIKNV
NSIRFVGQAIEYEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKAHLPE
LPDDKKERFVRDLGLSVYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNKAGKPIEGTPVSPAQLGGIIDL
IKDGTISGKLAKDLFEIVWNEGGDPAQIVESRGMKQVTDTGAIEKAVDEIIAANPDQVEKAKAKPSLAGWFVGQVMKATG
GKANPQAVQALVKSKLGFEE

Sequences:

>Translated_500_residues
MSIVDVRTPDPKRFIPGATGDWEVIIGMEVHAQVLSNSKLFSGASTEFGSEPNANVSLVDAAMPGMLPVINEECVKQAVR
TGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQYKDPIVGEGKIIISVGPDRQGHFEDVEIGIERLHLEQDAGKSMHDQH
PAMSYVDLNRSGVALMEIVSKPDLRSSDEAKAYLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEAFGTRCEIKNV
NSIRFVGQAIEYEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKAHLPE
LPDDKKERFVRDLGLSVYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNKAGKPIEGTPVSPAQLGGIIDL
IKDGTISGKLAKDLFEIVWNEGGDPAQIVESRGMKQVTDTGAIEKAVDEIIAANPDQVEKAKAKPSLAGWFVGQVMKATG
GKANPQAVQALVKSKLGFEE
>Mature_499_residues
SIVDVRTPDPKRFIPGATGDWEVIIGMEVHAQVLSNSKLFSGASTEFGSEPNANVSLVDAAMPGMLPVINEECVKQAVRT
GLGLKAKINNRSIFDRKNYFYPDLPQGYQISQYKDPIVGEGKIIISVGPDRQGHFEDVEIGIERLHLEQDAGKSMHDQHP
AMSYVDLNRSGVALMEIVSKPDLRSSDEAKAYLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEAFGTRCEIKNVN
SIRFVGQAIEYEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKAHLPEL
PDDKKERFVRDLGLSVYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNKAGKPIEGTPVSPAQLGGIIDLI
KDGTISGKLAKDLFEIVWNEGGDPAQIVESRGMKQVTDTGAIEKAVDEIIAANPDQVEKAKAKPSLAGWFVGQVMKATGG
KANPQAVQALVKSKLGFEE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=40.4040404040404, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI115534094, Length=300, Percent_Identity=41, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=529, Percent_Identity=29.8676748582231, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=37.319587628866, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54590; Mature: 54459

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVDVRTPDPKRFIPGATGDWEVIIGMEVHAQVLSNSKLFSGASTEFGSEPNANVSLVD
CCEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAVRTGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQYKDPIVG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCC
EGKIIISVGPDRQGHFEDVEIGIERLHLEQDAGKSMHDQHPAMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHC
KPDLRSSDEAKAYLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEAFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHCCEEEECCC
NSIRFVGQAIEYEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVEALKAHLPELPDDKKERFVRDLGLSVYDASVLVSEKAIADYFEAVAAGR
CEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCC
DGKTAANWVINDLLGALNKAGKPIEGTPVSPAQLGGIIDLIKDGTISGKLAKDLFEIVWN
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
EGGDPAQIVESRGMKQVTDTGAIEKAVDEIIAANPDQVEKAKAKPSLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVQALVKSKLGFEE
CCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIVDVRTPDPKRFIPGATGDWEVIIGMEVHAQVLSNSKLFSGASTEFGSEPNANVSLVD
CEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAVRTGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQYKDPIVG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCC
EGKIIISVGPDRQGHFEDVEIGIERLHLEQDAGKSMHDQHPAMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHC
KPDLRSSDEAKAYLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEAFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHCCEEEECCC
NSIRFVGQAIEYEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVEALKAHLPELPDDKKERFVRDLGLSVYDASVLVSEKAIADYFEAVAAGR
CEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCC
DGKTAANWVINDLLGALNKAGKPIEGTPVSPAQLGGIIDLIKDGTISGKLAKDLFEIVWN
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
EGGDPAQIVESRGMKQVTDTGAIEKAVDEIIAANPDQVEKAKAKPSLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVQALVKSKLGFEE
CCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]