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The map label for this gene is gatA [H]

Identifier: 150396167

GI number: 150396167

Start: 1019935

End: 1021416

Strand: Direct

Name: gatA [H]

Synonym: Smed_0944

Alternate gene names: 150396167

Gene position: 1019935-1021416 (Clockwise)

Preceding gene: 150396166

Following gene: 150396169

Centisome position: 26.97

GC content: 63.09

Gene sequence:

>1482_bases
ATGACCGACCTTACGAGCCTCACGATCGCAGAAGCCCGCGCGAAGCTTTCCGACAAGGAAATCACCGCGGTCGAGTTGAC
CGACGCCTATCTTGCCGCGATCGAGGCAGCCAATGAGACGATCAACGCCTATATCGCGGTCACGCCCGATAAGGCACGCG
AGATGGCAAAAGCCTCTGACGCGCGCATCGGGGCCGGCAAGGCGGGTGCGCTCGAGGGTATACCGCTCGGGATCAAGGAT
CTCTTCGGCACGCAGGGCCTCCATACGCAGGCGTGCAGCCATATACTCGACGGGTTCAGGCCGCACTATGAATCAACTGT
CACCCAGAACCTGTGGAACGACGGCGCCGTCATGCTCGGCAAGCTGAACATGGACGAGTTCGCGATGGGCTCGTCGAACG
AGACCTCCTATTACGGGCCGGTCAAGAACCCGTGGCGTGCCAAGGAATCCAATCTCGACCTGGTTCCGGGCGGGTCTTCC
GGCGGTTCTGCGGCTGCGGTCGCTGCACGCCTGTGCGCCGGAGCCACGGCGACGGACACCGGTGGCTCCATCCGCCAGCC
GGCGGCATTCACCGGAACCGTCGGCATCAAGCCGACCTATGGGCGCTGCTCGCGCTGGGGCGTGATCGCCTTCGCGTCGT
CGCTCGATCAGGCGGGCCCGATCGCTCGCGACGTGCGCGATGCCGCGATCCTCCTGAAGTCGATGGCGAGCGTCGATCCG
AAGGATACGACATCCGTCGATCTGCCGGTGCCGGATTACGAAGCGGCGATCGGCCAGTCGATCAAGGGCATGCGGATAGG
GATCCCGAAGGAATACCGCGTCGAAGGCATGCCCGAGGAAATCGAGGCGCTCTGGCAGCAGGGCGTTGCTTGGCTCAAGG
ACGCCGGCGCCGAAATCGTCGATATCTCGCTGCCGCATACCAAATACGCGCTGCCCGCCTACTACATCGTCGCTCCAGCC
GAAGCCTCGTCCAACCTCGCGCGCTACGACGGCGTTCGTTATGGCCTTCGCGTCGACGGCAAGGACATCGTCGACATGTA
CGAGAAGACGCGGGCGGCGGGCTTCGGGCAAGAGGTCAAGCGCCGCATCATGATCGGCACCTATGTTCTCTCTGCGGGAT
ATTACGATGCCTATTACCTTCGCGCGCAGAAAGTCCGCACCCTGATCAAGCGCGACTTCGAACTCGCCTTCCACGCCGGC
GTCGATGCTATCCTGACGCCGGCCACGCCGTCCTCCGCCTTCGGCATCGCCGATGAAGACCTCGCATCAGACCCGGTGAA
GATGTATCTGAACGACATCTTTACCGTGACGGTGAACATGGCCGGGCTTCCGGGCATCGCAGTGCCGGGCGGGCTCGATC
ACAAGGGGTTGCCGCTCGGTTTGCAGCTCATCGGCAAACCCTTCGACGAAGAAACGCTGTTCAAGACGGCTCACGTCATC
GAGCAGGCGGCTGGCCGCTTCACGCCCTCCAAATGGTGGTGA

Upstream 100 bases:

>100_bases
GCACCCAATTCGGACCGCAATTTCTTCGTCGTCCCGAAGGTGGTCGAGTAACCGTCCGCCGCCCGCCCAGTCGTCCCGAT
TGCGAAAGTCCAAATACGCC

Downstream 100 bases:

>100_bases
CCGCGCAGGGCGGCCATAAGCCGGCGGTAATCCCGCCGCGACGGCCAGCCCTGGTCTGGGATACCGGAGACGAGATCTCC
GGTATCCCATTGGATCGCCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTDLTSLTIAEARAKLSDKEITAVELTDAYLAAIEAANETINAYIAVTPDKAREMAKASDARIGAGKAGALEGIPLGIKD
LFGTQGLHTQACSHILDGFRPHYESTVTQNLWNDGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKESNLDLVPGGSS
GGSAAAVAARLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVIAFASSLDQAGPIARDVRDAAILLKSMASVDP
KDTTSVDLPVPDYEAAIGQSIKGMRIGIPKEYRVEGMPEEIEALWQQGVAWLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGQEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFELAFHAG
VDAILTPATPSSAFGIADEDLASDPVKMYLNDIFTVTVNMAGLPGIAVPGGLDHKGLPLGLQLIGKPFDEETLFKTAHVI
EQAAGRFTPSKWW

Sequences:

>Translated_493_residues
MTDLTSLTIAEARAKLSDKEITAVELTDAYLAAIEAANETINAYIAVTPDKAREMAKASDARIGAGKAGALEGIPLGIKD
LFGTQGLHTQACSHILDGFRPHYESTVTQNLWNDGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKESNLDLVPGGSS
GGSAAAVAARLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVIAFASSLDQAGPIARDVRDAAILLKSMASVDP
KDTTSVDLPVPDYEAAIGQSIKGMRIGIPKEYRVEGMPEEIEALWQQGVAWLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGQEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFELAFHAG
VDAILTPATPSSAFGIADEDLASDPVKMYLNDIFTVTVNMAGLPGIAVPGGLDHKGLPLGLQLIGKPFDEETLFKTAHVI
EQAAGRFTPSKWW
>Mature_492_residues
TDLTSLTIAEARAKLSDKEITAVELTDAYLAAIEAANETINAYIAVTPDKAREMAKASDARIGAGKAGALEGIPLGIKDL
FGTQGLHTQACSHILDGFRPHYESTVTQNLWNDGAVMLGKLNMDEFAMGSSNETSYYGPVKNPWRAKESNLDLVPGGSSG
GSAAAVAARLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVIAFASSLDQAGPIARDVRDAAILLKSMASVDPK
DTTSVDLPVPDYEAAIGQSIKGMRIGIPKEYRVEGMPEEIEALWQQGVAWLKDAGAEIVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGQEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFELAFHAGV
DAILTPATPSSAFGIADEDLASDPVKMYLNDIFTVTVNMAGLPGIAVPGGLDHKGLPLGLQLIGKPFDEETLFKTAHVIE
QAAGRFTPSKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=41.2955465587045, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=25.3036437246964, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI166795287, Length=257, Percent_Identity=24.9027237354086, Blast_Score=66, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=37.4717832957111, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=512, Percent_Identity=24.8046875, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17538252, Length=269, Percent_Identity=25.6505576208178, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=191, Percent_Identity=30.3664921465969, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=539, Percent_Identity=23.747680890538, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=425, Percent_Identity=36.9411764705882, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=411, Percent_Identity=27.4939172749392, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=26.8595041322314, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=38.4458077709611, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=23.9043824701195, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652985, Length=511, Percent_Identity=24.4618395303327, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922090, Length=511, Percent_Identity=24.4618395303327, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652981, Length=511, Percent_Identity=24.4618395303327, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652983, Length=511, Percent_Identity=24.4618395303327, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356731, Length=512, Percent_Identity=24.609375, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550774, Length=255, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648435, Length=255, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648437, Length=255, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161078093, Length=266, Percent_Identity=27.0676691729323, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52752; Mature: 52621

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTSLTIAEARAKLSDKEITAVELTDAYLAAIEAANETINAYIAVTPDKAREMAKASD
CCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCC
ARIGAGKAGALEGIPLGIKDLFGTQGLHTQACSHILDGFRPHYESTVTQNLWNDGAVMLG
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKESNLDLVPGGSSGGSAAAVAARLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRWGVIAFASSLDQAGPIARDVRDAAILLKSMASVDP
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KDTTSVDLPVPDYEAAIGQSIKGMRIGIPKEYRVEGMPEEIEALWQQGVAWLKDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGQEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFELAFHAGVDAILTPATPSSAFGIADED
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHH
LASDPVKMYLNDIFTVTVNMAGLPGIAVPGGLDHKGLPLGLQLIGKPFDEETLFKTAHVI
HCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
EQAAGRFTPSKWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
TDLTSLTIAEARAKLSDKEITAVELTDAYLAAIEAANETINAYIAVTPDKAREMAKASD
CCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCC
ARIGAGKAGALEGIPLGIKDLFGTQGLHTQACSHILDGFRPHYESTVTQNLWNDGAVMLG
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSSNETSYYGPVKNPWRAKESNLDLVPGGSSGGSAAAVAARLCAGATATDT
ECCHHHHCCCCCCCCCEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRWGVIAFASSLDQAGPIARDVRDAAILLKSMASVDP
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KDTTSVDLPVPDYEAAIGQSIKGMRIGIPKEYRVEGMPEEIEALWQQGVAWLKDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGQEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFELAFHAGVDAILTPATPSSAFGIADED
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHH
LASDPVKMYLNDIFTVTVNMAGLPGIAVPGGLDHKGLPLGLQLIGKPFDEETLFKTAHVI
HCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
EQAAGRFTPSKWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]