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The map label for this gene is pleD [H]

Identifier: 150396149

GI number: 150396149

Start: 1000965

End: 1002332

Strand: Direct

Name: pleD [H]

Synonym: Smed_0926

Alternate gene names: 150396149

Gene position: 1000965-1002332 (Clockwise)

Preceding gene: 150396148

Following gene: 150396160

Centisome position: 26.47

GC content: 60.89

Gene sequence:

>1368_bases
ATGACTGCGCGTATCCTCGTCGTTGATGACGTACCAGCCAACGTGAAGCTATTGGAAGCGCGGCTGGTTGCTGAATATTT
CGAAGTGCTGACGGCTGCAGACGGACACGCAGCGCTGGCTATTTGCGAACAGACGTCCGTCGACCTCGTTCTGCTCGATA
TCATGATGCCTCATATGGATGGGTTCGAGGTTTGCGAAAGGCTCAAGGCCAGCAGCCGCACCGCGCATATTCCGGTGGTG
ATGATAACCGCGTTAGACCAGCCGTCCGACCGTGTGCGCGGGCTGAAAGCCGGTGCGGACGACTTTCTGACAAAACCGGT
CAACGATCTGCAGCTCATGTCCCGCGTAAAGAGCCTCGTGCGTTTGAAGAACGTCAGCGACGAGCTGCGTCTCAGGGCGC
AGACGGCACAGACGATCGGATTGCAGGAGTTGAGTCGCCTTGATCGGCCCGACGAGCCCGGCAGCGTTCTGCTGGTCGAT
GGCCGCGCATCCTCGCAGGAGCGGCTCACTCGCGCTCTGAGGCCCATCGCCGATGTTGCTGTCCTGTCGGATGCGCAGGC
TGCTCTCTTCGAGGCCGCCGAGAACAGTTTCGACCTTGTCATCGTCAATGCGAACTTCGACGATTACGATCCGCTACGTC
TGTGTTCGCAATTGCGCTCGCTCGAGCGGACCCGCTTCATTCCCATCCTGCTGGTTACCGAACAGGGAAACGACGAGATG
ATCGTCCGTGCACTCGAACTGGGCGTGACGGATTACATCATGCGCCCCGTCGATCCGAACGAACTTGTCGCCCGCTCCCT
CACGCAGATCCGGCGCAAGCACTGCAATGACCGCTTGCGCGCAAGTGTTCAGCAGACGATCGAGTTGGCAGTGACCGATG
ATCTCACCGGTCTTCACAACCGGCGCTATCTGGAGAATCACTTGAAACTGCTGATCGATCGTGCGGGCGCCCGAGGGCGT
CCGCTGTCGATCTGCATTACCGATATCGATCGCTTCAAGCGTGTGAACGACACCTACGGACATGATGCCGGCGACGACGT
GCTGCGCGAGTTCGCAAACCGAGTCCGCGCAACGGTGCGTGGCGCGGACCTCGCCTGCCGGTTCGGTGGGGAGGAGTTCG
TCGTCGTCATGCCGGACACGACACCTGAAATGGCGGCAATCGTTGCCGAGAGGCTTCGGCTGTCAGTCGAGAGTCGCGGG
TTCGACATTGCGGAGGCTGCGACGGTACTGACAGTCACCGCGTCGCTCGGCATAGCGAGCCTGAGGCCGGACGGCGACAC
GGCAGAGGCTCTGCTGAAAAGGGCCGACATGGCGCTCTACGAGGCAAAGAACGGCGGGCGCAATCGTGTCGTCGCAGCCG
CGGCCTGA

Upstream 100 bases:

>100_bases
GAGGATGCGAGGCCTATGTTTCCAAGCCGATTTCCGTTCCCAAATTTATTGAAACGATCAAAACATATCTGGGCGATGCC
TGAGCGCCGGAAAGATCGAT

Downstream 100 bases:

>100_bases
ATTTTCATAAAGCGATCTGGAGGCAATCGGTGGCCGTCGAGCCCTATTGGCGCTCTTGCACGGTCGCCCTTTCGCATCGA
GCAGGAGGCGCTGTTCGATT

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTARILVVDDVPANVKLLEARLVAEYFEVLTAADGHAALAICEQTSVDLVLLDIMMPHMDGFEVCERLKASSRTAHIPVV
MITALDQPSDRVRGLKAGADDFLTKPVNDLQLMSRVKSLVRLKNVSDELRLRAQTAQTIGLQELSRLDRPDEPGSVLLVD
GRASSQERLTRALRPIADVAVLSDAQAALFEAAENSFDLVIVNANFDDYDPLRLCSQLRSLERTRFIPILLVTEQGNDEM
IVRALELGVTDYIMRPVDPNELVARSLTQIRRKHCNDRLRASVQQTIELAVTDDLTGLHNRRYLENHLKLLIDRAGARGR
PLSICITDIDRFKRVNDTYGHDAGDDVLREFANRVRATVRGADLACRFGGEEFVVVMPDTTPEMAAIVAERLRLSVESRG
FDIAEAATVLTVTASLGIASLRPDGDTAEALLKRADMALYEAKNGGRNRVVAAAA

Sequences:

>Translated_455_residues
MTARILVVDDVPANVKLLEARLVAEYFEVLTAADGHAALAICEQTSVDLVLLDIMMPHMDGFEVCERLKASSRTAHIPVV
MITALDQPSDRVRGLKAGADDFLTKPVNDLQLMSRVKSLVRLKNVSDELRLRAQTAQTIGLQELSRLDRPDEPGSVLLVD
GRASSQERLTRALRPIADVAVLSDAQAALFEAAENSFDLVIVNANFDDYDPLRLCSQLRSLERTRFIPILLVTEQGNDEM
IVRALELGVTDYIMRPVDPNELVARSLTQIRRKHCNDRLRASVQQTIELAVTDDLTGLHNRRYLENHLKLLIDRAGARGR
PLSICITDIDRFKRVNDTYGHDAGDDVLREFANRVRATVRGADLACRFGGEEFVVVMPDTTPEMAAIVAERLRLSVESRG
FDIAEAATVLTVTASLGIASLRPDGDTAEALLKRADMALYEAKNGGRNRVVAAAA
>Mature_454_residues
TARILVVDDVPANVKLLEARLVAEYFEVLTAADGHAALAICEQTSVDLVLLDIMMPHMDGFEVCERLKASSRTAHIPVVM
ITALDQPSDRVRGLKAGADDFLTKPVNDLQLMSRVKSLVRLKNVSDELRLRAQTAQTIGLQELSRLDRPDEPGSVLLVDG
RASSQERLTRALRPIADVAVLSDAQAALFEAAENSFDLVIVNANFDDYDPLRLCSQLRSLERTRFIPILLVTEQGNDEMI
VRALELGVTDYIMRPVDPNELVARSLTQIRRKHCNDRLRASVQQTIELAVTDDLTGLHNRRYLENHLKLLIDRAGARGRP
LSICITDIDRFKRVNDTYGHDAGDDVLREFANRVRATVRGADLACRFGGEEFVVVMPDTTPEMAAIVAERLRLSVESRGF
DIAEAATVLTVTASLGIASLRPDGDTAEALLKRADMALYEAKNGGRNRVVAAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=203, Percent_Identity=37.4384236453202, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=39.1566265060241, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082007, Length=190, Percent_Identity=40, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=39.873417721519, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=37.4269005847953, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081881, Length=258, Percent_Identity=32.1705426356589, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=36.6459627329193, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=32.9545454545455, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.6459627329193, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=39.6694214876033, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=30.8176100628931, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=32.183908045977, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788394, Length=129, Percent_Identity=37.2093023255814, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=35.3293413173653, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=36.4406779661017, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.0508474576271, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.9341317365269, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=39.2, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=30.9090909090909, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=32.2033898305085, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=38.8349514563107, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790863, Length=133, Percent_Identity=31.5789473684211, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50170; Mature: 50039

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDVPANVKLLEARLVAEYFEVLTAADGHAALAICEQTSVDLVLLDIMMPHMD
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHHCCCC
GFEVCERLKASSRTAHIPVVMITALDQPSDRVRGLKAGADDFLTKPVNDLQLMSRVKSLV
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKNVSDELRLRAQTAQTIGLQELSRLDRPDEPGSVLLVDGRASSQERLTRALRPIADVA
HHHCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VLSDAQAALFEAAENSFDLVIVNANFDDYDPLRLCSQLRSLERTRFIPILLVTEQGNDEM
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHH
IVRALELGVTDYIMRPVDPNELVARSLTQIRRKHCNDRLRASVQQTIELAVTDDLTGLHN
HHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCCCCHHH
RRYLENHLKLLIDRAGARGRPLSICITDIDRFKRVNDTYGHDAGDDVLREFANRVRATVR
HHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
GADLACRFGGEEFVVVMPDTTPEMAAIVAERLRLSVESRGFDIAEAATVLTVTASLGIAS
CCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEHHHCCCEE
LRPDGDTAEALLKRADMALYEAKNGGRNRVVAAAA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
TARILVVDDVPANVKLLEARLVAEYFEVLTAADGHAALAICEQTSVDLVLLDIMMPHMD
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHHCCCC
GFEVCERLKASSRTAHIPVVMITALDQPSDRVRGLKAGADDFLTKPVNDLQLMSRVKSLV
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKNVSDELRLRAQTAQTIGLQELSRLDRPDEPGSVLLVDGRASSQERLTRALRPIADVA
HHHCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VLSDAQAALFEAAENSFDLVIVNANFDDYDPLRLCSQLRSLERTRFIPILLVTEQGNDEM
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCHH
IVRALELGVTDYIMRPVDPNELVARSLTQIRRKHCNDRLRASVQQTIELAVTDDLTGLHN
HHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCCCCHHH
RRYLENHLKLLIDRAGARGRPLSICITDIDRFKRVNDTYGHDAGDDVLREFANRVRATVR
HHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
GADLACRFGGEEFVVVMPDTTPEMAAIVAERLRLSVESRGFDIAEAATVLTVTASLGIAS
CCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEHHHCCCEE
LRPDGDTAEALLKRADMALYEAKNGGRNRVVAAAA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA