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The map label for this gene is mdeA [H]

Identifier: 150396090

GI number: 150396090

Start: 924979

End: 926259

Strand: Direct

Name: mdeA [H]

Synonym: Smed_0867

Alternate gene names: 150396090

Gene position: 924979-926259 (Clockwise)

Preceding gene: 150396089

Following gene: 150396091

Centisome position: 24.46

GC content: 61.83

Gene sequence:

>1281_bases
ATGAATGGCGGACCCGGTTTCAGCACGCTTGCAATTCATGCCGGAGCCCAGCCGGACCCGACGACCGGGGCGCGGGCGAC
GCCGATCTATCAGACGACCAGCTTCGTTTTCAATGACACGGATCATGCCGCCGCGCTCTTCGGCCTCAAGCAATTCGGCA
ATATCTATACCCGCATCATGAACCCGACGCAGGCGGTGCTGGAGGAGCGGATCGCGGCTCTTGAAGGCGGAACTGCCGGG
CTTGCGGTTTCTTCGGGGCATGCGGCCCAGCTGCTCGTTTTCCATACGATCATGAGCCCGGGTGACAACTTCGTTTCGGC
CCGGCAGCTATACGGCGGCTCCGTCAACCAGTTCGGCCAGTCCTTCAAGGCATTCGATTGGCAGGTCCGCTGGGCCGATT
CAGCGGAACCCGAAAGCTTCGATGCGCATATCGACGAACGTACCAAAGCGATCTTCATCGAAAGCCTCGCCAATCCGGGC
GGCACTTTCGTCGATATAGCCGCGATCGCCGAAGTCGCGCGCCGGCACGGGCTGCCGCTCATCGTCGACAATACGATGGC
AACGCCTTATCTGGTGCGACCGCTGGAACATGGCGCCGATATCGTCGTCCACTCGCTGACCAAATTCATCGGTGGCCATG
GCAATTCGATGGGCGGCATGATCGTCGACGGCGGTACGTTCGACTGGTCGACATCAGGCAAGTATCCGCTGTTGTCGGAG
CCCAGGCCGGAATATGGCGGCGTCGTCCTGCACCAGGCCTTCGGCAACTTCGCTTTCGCCATCGCGGCGAGGGTGCTGGG
TTTGCGGGACTTCGGTCCGGCGATCTCTCCCTTCAACGCGTTTTTGATCCAGACCGGCGTCGAGACCCTGCCGCTCCGGA
TGCAGCGCCATTGCGACAACGCAGCTGAGGTTGCCAAGTGGCTGAAGGGGCACGAGAAGGTGTCCTGGGTTCGTTACGCC
GGTCTTGAAGACGATCCGAACAATGCGCTGCAGAAGCGCTATTCGCCGAAGGGAGCGGGAGCGGTCTTCACCTTCGGCCT
CGCTGGCGGATACGAGGCGGGAAAGCGCTTCGTCGAGGCGCTGGAAATGTTTTCCCATCTCGCCAATATCGGCGACACAC
GTTCGCTCGTGATCCATCCCGCCTCGACCACCCACCGGCAACTCACACCGGAACAGCAGACCGCGGCGGGGGCCGGGCCC
GACGTCATCCGGTTGTCGGTCGGAATTGAGGATATCGTCGACATCATCGCCGACCTCGAGCAGGCGCTGGCAAAGGCTTG
A

Upstream 100 bases:

>100_bases
GCAGGCATCAAGGTCGTCATGGATCGCTGCCCGGCCATCGAATATCCGCGCCTGATCGGATGAGCATCAGATTATTCAAG
TGAGTGAGGGAGAAACGAAG

Downstream 100 bases:

>100_bases
GCCGCACTGCAGGAGCGGAGGCTTCGATGAGCCATATGCTGAAATTCGATTTGTCTTCGGTCGGGGCCGAGGTCGGCGCT
CCGGCGCCGGACCGCCTGAT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNGGPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLKQFGNIYTRIMNPTQAVLEERIAALEGGTAG
LAVSSGHAAQLLVFHTIMSPGDNFVSARQLYGGSVNQFGQSFKAFDWQVRWADSAEPESFDAHIDERTKAIFIESLANPG
GTFVDIAAIAEVARRHGLPLIVDNTMATPYLVRPLEHGADIVVHSLTKFIGGHGNSMGGMIVDGGTFDWSTSGKYPLLSE
PRPEYGGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDNAAEVAKWLKGHEKVSWVRYA
GLEDDPNNALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEALEMFSHLANIGDTRSLVIHPASTTHRQLTPEQQTAAGAGP
DVIRLSVGIEDIVDIIADLEQALAKA

Sequences:

>Translated_426_residues
MNGGPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLKQFGNIYTRIMNPTQAVLEERIAALEGGTAG
LAVSSGHAAQLLVFHTIMSPGDNFVSARQLYGGSVNQFGQSFKAFDWQVRWADSAEPESFDAHIDERTKAIFIESLANPG
GTFVDIAAIAEVARRHGLPLIVDNTMATPYLVRPLEHGADIVVHSLTKFIGGHGNSMGGMIVDGGTFDWSTSGKYPLLSE
PRPEYGGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDNAAEVAKWLKGHEKVSWVRYA
GLEDDPNNALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEALEMFSHLANIGDTRSLVIHPASTTHRQLTPEQQTAAGAGP
DVIRLSVGIEDIVDIIADLEQALAKA
>Mature_426_residues
MNGGPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLKQFGNIYTRIMNPTQAVLEERIAALEGGTAG
LAVSSGHAAQLLVFHTIMSPGDNFVSARQLYGGSVNQFGQSFKAFDWQVRWADSAEPESFDAHIDERTKAIFIESLANPG
GTFVDIAAIAEVARRHGLPLIVDNTMATPYLVRPLEHGADIVVHSLTKFIGGHGNSMGGMIVDGGTFDWSTSGKYPLLSE
PRPEYGGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDNAAEVAKWLKGHEKVSWVRYA
GLEDDPNNALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEALEMFSHLANIGDTRSLVIHPASTTHRQLTPEQQTAAGAGP
DVIRLSVGIEDIVDIIADLEQALAKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=30.9692671394799, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=28.3687943262411, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=27.4881516587678, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1790375, Length=419, Percent_Identity=30.0715990453461, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=21.9626168224299, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71980708, Length=419, Percent_Identity=29.5942720763723, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=32.3185011709602, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=30.9859154929577, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=46.5277777777778, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=30.9859154929577, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=27.1910112359551, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=369, Percent_Identity=24.9322493224932, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=32.0843091334895, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45629; Mature: 45629

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGGPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLKQFGNIYTRIM
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
NPTQAVLEERIAALEGGTAGLAVSSGHAAQLLVFHTIMSPGDNFVSARQLYGGSVNQFGQ
CHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
SFKAFDWQVRWADSAEPESFDAHIDERTKAIFIESLANPGGTFVDIAAIAEVARRHGLPL
HHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCE
IVDNTMATPYLVRPLEHGADIVVHSLTKFIGGHGNSMGGMIVDGGTFDWSTSGKYPLLSE
EEECCCCCCHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
PRPEYGGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDN
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
AAEVAKWLKGHEKVSWVRYAGLEDDPNNALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEA
HHHHHHHHHCCHHEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
LEMFSHLANIGDTRSLVIHPASTTHRQLTPEQQTAAGAGPDVIRLSVGIEDIVDIIADLE
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHH
QALAKA
HHHHCC
>Mature Secondary Structure
MNGGPGFSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLKQFGNIYTRIM
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
NPTQAVLEERIAALEGGTAGLAVSSGHAAQLLVFHTIMSPGDNFVSARQLYGGSVNQFGQ
CHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
SFKAFDWQVRWADSAEPESFDAHIDERTKAIFIESLANPGGTFVDIAAIAEVARRHGLPL
HHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCE
IVDNTMATPYLVRPLEHGADIVVHSLTKFIGGHGNSMGGMIVDGGTFDWSTSGKYPLLSE
EEECCCCCCHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
PRPEYGGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDN
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
AAEVAKWLKGHEKVSWVRYAGLEDDPNNALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEA
HHHHHHHHHCCHHEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHH
LEMFSHLANIGDTRSLVIHPASTTHRQLTPEQQTAAGAGPDVIRLSVGIEDIVDIIADLE
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHH
QALAKA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]