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The map label for this gene is yoaE [H]

Identifier: 150396013

GI number: 150396013

Start: 845808

End: 847940

Strand: Direct

Name: yoaE [H]

Synonym: Smed_0789

Alternate gene names: 150396013

Gene position: 845808-847940 (Clockwise)

Preceding gene: 150396008

Following gene: 150396014

Centisome position: 22.36

GC content: 63.34

Gene sequence:

>2133_bases
ATGCCATATAGAAAAGCCATGAACGTTGCGAGCCCCGTCAGACAAGAAAAGACCATCGGCCATTCGGTCTGTCCACATGA
CTGTCCCTCCGCGTGCGCGCTCGACATCGACCTCACGGCCGAGGGTCGGATCGGGCGTGTGCGCGGCGCGGCGGAAAACA
CCTATACGGCCGGGGTCATCTGCGCCAAGGTCGCGCGCTATTCCGAGCGCATCTACCATCCCGACCGACTCATGGTGCCA
CAACGCCGGACGAGTGCGAAGGGGGAGGGCCGCTGGCAGGAGATCACCTGGGAAGCGGCACTCGACGAGATCGCCGCCCA
TTTCGTGAAGGCCGAGCAGAAATACGGCTCGGAGTCGGTCTGGCCCTATTTCTATGCCGGAACCATGGGGCAGGTACAGC
GCGACTCCATAGAAAGATTGCGCCACGCCAAACGCTATTCCGGTTTCTTCGGGTCGATCTGCACCAATATGGCGTGGACC
GGCGTCTCGATGGCGACCGGAGCCTTGCGCGGCCCTGACCCGAGGGAAATGGCGAAGTCCGATTGCGTGGTGATATGGGG
TACCAATGCGGTGGCGACACAGGTCAACGTGATGACCCACGCAGTCAAAGCGCGCAAGGATCGGGGCGCCAAGATCGTCG
TTATCGACGTCTACGACAATCCGACGATCAAGCAGGCCGATATGGGGCTGGTGCTGAAACCCGGCACCGACGCGGCGCTC
GCTTGCGCCGTCATGCACATCGCCTTTCGCGACGGCTATGCCGACCGTGCTTACATGGCAAAATTCGCTGACGATCCCGC
GGGGCTCGAGGCGCATCTCAAAACACGGGGGCCGGAATGGGCCTCGGCGATTACCGGGCTTCCAATAGAGGAGATCGAGG
CTTTCGCCAGGCTCGTCGGGACCACGCCCCGGACCTATTTCCGTCTGGGTTACGGCTTCACGCGCCAGAGAAACGGGGCG
GTTGCGATCCATGCCGCAGCTTCGATCGCCACGGTGCTCGGCTCCTGGAAACACGAGGGCGGCGGCGCCTTCCATTCCAA
CAACGACATCTTCAGGCTCGACAAGCGCGAACTCGTCGGCACTGCGCTGCACGATCCGGAAGTGCGCATGCTCGACCAGT
CGCAGATCGGCCGCGTGCTGACCGGAGACGCCGAGGCGCTTCGCCATCGCGGTCCCGTGACGGCGCTGCTCATCCAGAAC
ACCAATCCGGTCAATGTCGCGCCCGAGCAGCGGCTGGTGAAGCGCGGCTTCCTGCGCGACGACCTCTTCATTGCCGTGCA
CGAGCATTTCATGACCGACACGGCGCAACTCGCGGACATCGTCCTGCCGGCTACCATGTTTCTCGAGCATGACGATCTCT
ATCGGGGTGGCGGCCACCAGCATATCCTGCTGGGGCCGAAGGTCGTCGAGCCGCCGCCGACCGTGCGCACCAATCTTTTC
GTCATCGAAGAGCTGGCAAAGCGTCTCGGCGTCGCAGACTGTCCCGGCTTCGGCCTGACGGAACGGCAGCATATCGACCG
GTTGCTCGCCAATTACGGCATCGGCTATGACGGGATGAAGGAGCGGAAATGGCTCGATTGCCAGCCGAATTTCGACGAGG
CGCACTTCCTCGATGGTTTCGGGCATCCGGACGGCAGATTCCGGTTCAAGGCGGATTGGACAGGCACGCCCGCGCCGAAC
CGGCCACCGAAGGCAATGGGTCCACAGGGGCCGCATGGTAAGCTTCCGGAGTTTCCCGATCACGTCGACCTCATCGAAGT
GGCCGACGAGCGGCATCCATTCCGGCTTGCAACGTCGCCGGCGCGATCCTTCCTCAATTCGACCTTCGCCGAAACTCCGT
CCTCGATCCAGAAGGAAGGCCGGCCGGAAGTCATGATTCATGCGGAGGATGCGGCCGAACTCGGTGTCGCCGAAGGCGAC
GTGGTCAGGCTGGGCAACGGGCGTGGTGAAATTCGCCTGCACGCGAAGATCGGTGGCGGCGCCCGTCGCGGCGTGGTCGT
CGCCGAAGGCCTTTGGCCGAACAGCGCCCACTTGGACGGGGAGGGGATCAACGTTTTGACCGGTGCCGATGCCGTCGCAC
CCTATGGCGGCGCTGCATTCCATGACAACCGGGTCTGGATGCGCCGGGCCTGA

Upstream 100 bases:

>100_bases
TTGCCCGCGTGATAGATGTGCCGGTAATTCATAAGCGTTGCCGATGGCCGCGACCAATTGTTGCGATGGATGGCGAATGG
GGCTTTGAGCGTCCCTGGCC

Downstream 100 bases:

>100_bases
CCGGTCCACTCTTCTGAAGGATTCTCCATGGTCAAGAATACAGACCCGCGCTTCCGGATCATCGACCGCAAAACGCTCTG
GGATGGTTTCATCAATCTCG

Product: nitrate reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MPYRKAMNVASPVRQEKTIGHSVCPHDCPSACALDIDLTAEGRIGRVRGAAENTYTAGVICAKVARYSERIYHPDRLMVP
QRRTSAKGEGRWQEITWEAALDEIAAHFVKAEQKYGSESVWPYFYAGTMGQVQRDSIERLRHAKRYSGFFGSICTNMAWT
GVSMATGALRGPDPREMAKSDCVVIWGTNAVATQVNVMTHAVKARKDRGAKIVVIDVYDNPTIKQADMGLVLKPGTDAAL
ACAVMHIAFRDGYADRAYMAKFADDPAGLEAHLKTRGPEWASAITGLPIEEIEAFARLVGTTPRTYFRLGYGFTRQRNGA
VAIHAAASIATVLGSWKHEGGGAFHSNNDIFRLDKRELVGTALHDPEVRMLDQSQIGRVLTGDAEALRHRGPVTALLIQN
TNPVNVAPEQRLVKRGFLRDDLFIAVHEHFMTDTAQLADIVLPATMFLEHDDLYRGGGHQHILLGPKVVEPPPTVRTNLF
VIEELAKRLGVADCPGFGLTERQHIDRLLANYGIGYDGMKERKWLDCQPNFDEAHFLDGFGHPDGRFRFKADWTGTPAPN
RPPKAMGPQGPHGKLPEFPDHVDLIEVADERHPFRLATSPARSFLNSTFAETPSSIQKEGRPEVMIHAEDAAELGVAEGD
VVRLGNGRGEIRLHAKIGGGARRGVVVAEGLWPNSAHLDGEGINVLTGADAVAPYGGAAFHDNRVWMRRA

Sequences:

>Translated_710_residues
MPYRKAMNVASPVRQEKTIGHSVCPHDCPSACALDIDLTAEGRIGRVRGAAENTYTAGVICAKVARYSERIYHPDRLMVP
QRRTSAKGEGRWQEITWEAALDEIAAHFVKAEQKYGSESVWPYFYAGTMGQVQRDSIERLRHAKRYSGFFGSICTNMAWT
GVSMATGALRGPDPREMAKSDCVVIWGTNAVATQVNVMTHAVKARKDRGAKIVVIDVYDNPTIKQADMGLVLKPGTDAAL
ACAVMHIAFRDGYADRAYMAKFADDPAGLEAHLKTRGPEWASAITGLPIEEIEAFARLVGTTPRTYFRLGYGFTRQRNGA
VAIHAAASIATVLGSWKHEGGGAFHSNNDIFRLDKRELVGTALHDPEVRMLDQSQIGRVLTGDAEALRHRGPVTALLIQN
TNPVNVAPEQRLVKRGFLRDDLFIAVHEHFMTDTAQLADIVLPATMFLEHDDLYRGGGHQHILLGPKVVEPPPTVRTNLF
VIEELAKRLGVADCPGFGLTERQHIDRLLANYGIGYDGMKERKWLDCQPNFDEAHFLDGFGHPDGRFRFKADWTGTPAPN
RPPKAMGPQGPHGKLPEFPDHVDLIEVADERHPFRLATSPARSFLNSTFAETPSSIQKEGRPEVMIHAEDAAELGVAEGD
VVRLGNGRGEIRLHAKIGGGARRGVVVAEGLWPNSAHLDGEGINVLTGADAVAPYGGAAFHDNRVWMRRA
>Mature_709_residues
PYRKAMNVASPVRQEKTIGHSVCPHDCPSACALDIDLTAEGRIGRVRGAAENTYTAGVICAKVARYSERIYHPDRLMVPQ
RRTSAKGEGRWQEITWEAALDEIAAHFVKAEQKYGSESVWPYFYAGTMGQVQRDSIERLRHAKRYSGFFGSICTNMAWTG
VSMATGALRGPDPREMAKSDCVVIWGTNAVATQVNVMTHAVKARKDRGAKIVVIDVYDNPTIKQADMGLVLKPGTDAALA
CAVMHIAFRDGYADRAYMAKFADDPAGLEAHLKTRGPEWASAITGLPIEEIEAFARLVGTTPRTYFRLGYGFTRQRNGAV
AIHAAASIATVLGSWKHEGGGAFHSNNDIFRLDKRELVGTALHDPEVRMLDQSQIGRVLTGDAEALRHRGPVTALLIQNT
NPVNVAPEQRLVKRGFLRDDLFIAVHEHFMTDTAQLADIVLPATMFLEHDDLYRGGGHQHILLGPKVVEPPPTVRTNLFV
IEELAKRLGVADCPGFGLTERQHIDRLLANYGIGYDGMKERKWLDCQPNFDEAHFLDGFGHPDGRFRFKADWTGTPAPNR
PPKAMGPQGPHGKLPEFPDHVDLIEVADERHPFRLATSPARSFLNSTFAETPSSIQKEGRPEVMIHAEDAAELGVAEGDV
VRLGNGRGEIRLHAKIGGGARRGVVVAEGLWPNSAHLDGEGINVLTGADAVAPYGGAAFHDNRVWMRRA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=792, Percent_Identity=26.1363636363636, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1787870, Length=761, Percent_Identity=26.9382391590013, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI171474008, Length=791, Percent_Identity=24.1466498103666, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI3868721, Length=704, Percent_Identity=24.1477272727273, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081994, Length=724, Percent_Identity=24.585635359116, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI145693196, Length=680, Percent_Identity=25.5882352941176, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787231, Length=677, Percent_Identity=22.0088626292467, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 77920; Mature: 77789

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRKAMNVASPVRQEKTIGHSVCPHDCPSACALDIDLTAEGRIGRVRGAAENTYTAGVI
CCHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHH
CAKVARYSERIYHPDRLMVPQRRTSAKGEGRWQEITWEAALDEIAAHFVKAEQKYGSESV
HHHHHHHHHHHCCCCCEECCCHHCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCCCC
WPYFYAGTMGQVQRDSIERLRHAKRYSGFFGSICTNMAWTGVSMATGALRGPDPREMAKS
CCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHCCC
DCVVIWGTNAVATQVNVMTHAVKARKDRGAKIVVIDVYDNPTIKQADMGLVLKPGTDAAL
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCEEECCCCCHHH
ACAVMHIAFRDGYADRAYMAKFADDPAGLEAHLKTRGPEWASAITGLPIEEIEAFARLVG
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHC
TTPRTYFRLGYGFTRQRNGAVAIHAAASIATVLGSWKHEGGGAFHSNNDIFRLDKRELVG
CCCHHEEEECCCEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHH
TALHDPEVRMLDQSQIGRVLTGDAEALRHRGPVTALLIQNTNPVNVAPEQRLVKRGFLRD
HHCCCCCCEEECHHHCCCEEECCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCC
DLFIAVHEHFMTDTAQLADIVLPATMFLEHDDLYRGGGHQHILLGPKVVEPPPTVRTNLF
CCEEEEHHHHHCCHHHHHHHHHHHHHEEECCCHHCCCCCEEEEECCEECCCCCCCCHHHH
VIEELAKRLGVADCPGFGLTERQHIDRLLANYGIGYDGMKERKWLDCQPNFDEAHFLDGF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCC
GHPDGRFRFKADWTGTPAPNRPPKAMGPQGPHGKLPEFPDHVDLIEVADERHPFRLATSP
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECHH
ARSFLNSTFAETPSSIQKEGRPEVMIHAEDAAELGVAEGDVVRLGNGRGEIRLHAKIGGG
HHHHHHHHHHCCCHHHHHCCCCCEEEEECCHHHCCCCCCCEEEECCCCCEEEEEEEECCC
ARRGVVVAEGLWPNSAHLDGEGINVLTGADAVAPYGGAAFHDNRVWMRRA
CCCCEEEECCCCCCCCEECCCCCEEEECCCCCCCCCCCEEECCEEEEECC
>Mature Secondary Structure 
PYRKAMNVASPVRQEKTIGHSVCPHDCPSACALDIDLTAEGRIGRVRGAAENTYTAGVI
CHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHH
CAKVARYSERIYHPDRLMVPQRRTSAKGEGRWQEITWEAALDEIAAHFVKAEQKYGSESV
HHHHHHHHHHHCCCCCEECCCHHCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCCCC
WPYFYAGTMGQVQRDSIERLRHAKRYSGFFGSICTNMAWTGVSMATGALRGPDPREMAKS
CCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHCCC
DCVVIWGTNAVATQVNVMTHAVKARKDRGAKIVVIDVYDNPTIKQADMGLVLKPGTDAAL
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCEEECCCCCHHH
ACAVMHIAFRDGYADRAYMAKFADDPAGLEAHLKTRGPEWASAITGLPIEEIEAFARLVG
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHC
TTPRTYFRLGYGFTRQRNGAVAIHAAASIATVLGSWKHEGGGAFHSNNDIFRLDKRELVG
CCCHHEEEECCCEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHH
TALHDPEVRMLDQSQIGRVLTGDAEALRHRGPVTALLIQNTNPVNVAPEQRLVKRGFLRD
HHCCCCCCEEECHHHCCCEEECCHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCC
DLFIAVHEHFMTDTAQLADIVLPATMFLEHDDLYRGGGHQHILLGPKVVEPPPTVRTNLF
CCEEEEHHHHHCCHHHHHHHHHHHHHEEECCCHHCCCCCEEEEECCEECCCCCCCCHHHH
VIEELAKRLGVADCPGFGLTERQHIDRLLANYGIGYDGMKERKWLDCQPNFDEAHFLDGF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCC
GHPDGRFRFKADWTGTPAPNRPPKAMGPQGPHGKLPEFPDHVDLIEVADERHPFRLATSP
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECHH
ARSFLNSTFAETPSSIQKEGRPEVMIHAEDAAELGVAEGDVVRLGNGRGEIRLHAKIGGG
HHHHHHHHHHCCCHHHHHCCCCCEEEEECCHHHCCCCCCCEEEECCCCCEEEEEEEECCC
ARRGVVVAEGLWPNSAHLDGEGINVLTGADAVAPYGGAAFHDNRVWMRRA
CCCCEEEECCCCCCCCEECCCCCEEEECCCCCCCCCCCEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]