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The map label for this gene is nadB [H]

Identifier: 150395924

GI number: 150395924

Start: 755648

End: 757246

Strand: Direct

Name: nadB [H]

Synonym: Smed_0700

Alternate gene names: 150395924

Gene position: 755648-757246 (Clockwise)

Preceding gene: 150395923

Following gene: 150395925

Centisome position: 19.98

GC content: 66.04

Gene sequence:

>1599_bases
ATGTTGACCGACCATTTTCGTCCGCAATCCTTCAACGGGATCGATGACATCGTCATCGTCGGCGGCGGCCTTGCAGGGCT
TTTCTGCGCGCTGAAGCTGGCGCCGCGTCCGGTCACGATCCTTGCCGCCGCACCGATCGGCTACGGCGCCTCCTCGGCAT
GGGCGCAAGGCGGGATCGCCGCGGCGATGAGCAAGGGCGATACATTCGACAAGCATGTCGCCGACACGGTCGCGGCCGGT
GCCGGTATCGTCGACGAGAAGATGACGCGCATGATGGTCGCCGAGGGCCCGGCCCGCATCCACGACCTGCTCGAATTCGG
AGTGCCCTTCGACCGCGATCTCGAGGGAAAGCTCATGCTCTCGCGGGAAGCCGCGCATTCCGAGCGACGCATCGTCCGTG
TCAAGGGCGATATGGCCGGTAAATCGATCATGGAGGCGTTGATTGCCGCGGTGCGCAATACTCCGTCCATCCGGGTGATC
GAGGGCTATGTTGTCGAGGAACTCGTGCGCGAGGGGCGGTTCATCTCCGGTGTCGTTGCACGGCCGGATGCCGGCCAGTC
GAAGAGCCGCGTTTCCTTCCCGGCGCGCGCCGTCGTTCTGTGCTCCGGCGGCGTCGGCCATCTCTATGCCGTCACGACCA
ATCCCTGGGAAGCCTGCGGTCAGGGCGTCGGCATGGCGGCGCGGGCGGGTGCCATCATCGCCGACCCCGAATTCGTTCAG
TTTCATCCGACCGCAATTGATATCGGCAAGGATCCAGCGCCGCTCGCCACCGAAGCCTTGCGCGGCGACGGCGCCATCCT
CATCAATGCGGAAGGCCGGCGCTTCATGCTCGACATCCACCCGGATGGTGAGCTTGCGCCGCGCGACATCGTCTCCCGCG
GCGTCTTCGCGGAAGTTCAGGCCGGACGCGGAGCCTTCCTCGATTGCACGAAGGCCGTCGGCAGGCATTTTCCCGACATG
TTCCCGACCGTTTACGCATCGTGCATGGCAGCGGGTATCGACCCGGTGTCACAGCCGATCCCCGTCGTCCCGGCCGTGCA
TTACCACATGGGCGGCGTGTTGACGGACGGGGAGGGGCGGACGTCCATCGACGGACTCTGGGCGGCTGGCGAAGTGACTT
CGACGGGCGTTCACGGCGCCAACCGCCTTGCCTCCAATTCGCTGCTCGAGGCGGTCGTATTCGCGGCGCGCATCGCGGAA
AACATCAAGGGCAGCTTGCCGGCGCCGAAGCTCACCTCCTGGGGCGACAATGCCGGCGAGAATGACGATCCCGTGACGGT
CGAAGACAGTCCGCCTTTCAAGCTGCTTCGGCAGCTGATGAGCGACTGCGTCGGAGTCGTGCGGACGCGCGAAAGCCTGA
CCCGCGCGATCCAGGGGATCACGCGGCTCGAGCGGGCCAACAAGCGACTTCGCTTCGCCAATATAGCCACCACGGCCAAG
CTCATAGCGGTCGCGGCGCTGCAGCGGACGGAAAGCCGCGGCGGTCATTTCCGCGCCGATTGCCCGGGTGAGCGGCCGGA
TTGGCAGCGCCGGACCTACCTGACGCTTGCCCAGGCGGAGCGCCTGGCCGTCGAAGTGGCCGAGAGCGAGCCGGCATGA

Upstream 100 bases:

>100_bases
TTCCGGCAGGCATGCCGAGGCATGCAGGGTTCAAATACTGCGGCAGATTTGCGTGTCAGAGAGGACGCGCGGCGCCGCAG
GTCTGGAGGAGTGCTGAGCC

Downstream 100 bases:

>100_bases
CGGCGGCAAAGGCGCAAACTGACACCCTTCCAACCGCAGGAACGACATCATGACCGCCCTTCGCCCCGAACTGCCCGTCC
TCATGCTCGAGGAGCAGGTG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLTDHFRPQSFNGIDDIVIVGGGLAGLFCALKLAPRPVTILAAAPIGYGASSAWAQGGIAAAMSKGDTFDKHVADTVAAG
AGIVDEKMTRMMVAEGPARIHDLLEFGVPFDRDLEGKLMLSREAAHSERRIVRVKGDMAGKSIMEALIAAVRNTPSIRVI
EGYVVEELVREGRFISGVVARPDAGQSKSRVSFPARAVVLCSGGVGHLYAVTTNPWEACGQGVGMAARAGAIIADPEFVQ
FHPTAIDIGKDPAPLATEALRGDGAILINAEGRRFMLDIHPDGELAPRDIVSRGVFAEVQAGRGAFLDCTKAVGRHFPDM
FPTVYASCMAAGIDPVSQPIPVVPAVHYHMGGVLTDGEGRTSIDGLWAAGEVTSTGVHGANRLASNSLLEAVVFAARIAE
NIKGSLPAPKLTSWGDNAGENDDPVTVEDSPPFKLLRQLMSDCVGVVRTRESLTRAIQGITRLERANKRLRFANIATTAK
LIAVAALQRTESRGGHFRADCPGERPDWQRRTYLTLAQAERLAVEVAESEPA

Sequences:

>Translated_532_residues
MLTDHFRPQSFNGIDDIVIVGGGLAGLFCALKLAPRPVTILAAAPIGYGASSAWAQGGIAAAMSKGDTFDKHVADTVAAG
AGIVDEKMTRMMVAEGPARIHDLLEFGVPFDRDLEGKLMLSREAAHSERRIVRVKGDMAGKSIMEALIAAVRNTPSIRVI
EGYVVEELVREGRFISGVVARPDAGQSKSRVSFPARAVVLCSGGVGHLYAVTTNPWEACGQGVGMAARAGAIIADPEFVQ
FHPTAIDIGKDPAPLATEALRGDGAILINAEGRRFMLDIHPDGELAPRDIVSRGVFAEVQAGRGAFLDCTKAVGRHFPDM
FPTVYASCMAAGIDPVSQPIPVVPAVHYHMGGVLTDGEGRTSIDGLWAAGEVTSTGVHGANRLASNSLLEAVVFAARIAE
NIKGSLPAPKLTSWGDNAGENDDPVTVEDSPPFKLLRQLMSDCVGVVRTRESLTRAIQGITRLERANKRLRFANIATTAK
LIAVAALQRTESRGGHFRADCPGERPDWQRRTYLTLAQAERLAVEVAESEPA
>Mature_532_residues
MLTDHFRPQSFNGIDDIVIVGGGLAGLFCALKLAPRPVTILAAAPIGYGASSAWAQGGIAAAMSKGDTFDKHVADTVAAG
AGIVDEKMTRMMVAEGPARIHDLLEFGVPFDRDLEGKLMLSREAAHSERRIVRVKGDMAGKSIMEALIAAVRNTPSIRVI
EGYVVEELVREGRFISGVVARPDAGQSKSRVSFPARAVVLCSGGVGHLYAVTTNPWEACGQGVGMAARAGAIIADPEFVQ
FHPTAIDIGKDPAPLATEALRGDGAILINAEGRRFMLDIHPDGELAPRDIVSRGVFAEVQAGRGAFLDCTKAVGRHFPDM
FPTVYASCMAAGIDPVSQPIPVVPAVHYHMGGVLTDGEGRTSIDGLWAAGEVTSTGVHGANRLASNSLLEAVVFAARIAE
NIKGSLPAPKLTSWGDNAGENDDPVTVEDSPPFKLLRQLMSDCVGVVRTRESLTRAIQGITRLERANKRLRFANIATTAK
LIAVAALQRTESRGGHFRADCPGERPDWQRRTYLTLAQAERLAVEVAESEPA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=501, Percent_Identity=31.7365269461078, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1788928, Length=515, Percent_Identity=39.6116504854369, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790597, Length=505, Percent_Identity=31.2871287128713, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786942, Length=541, Percent_Identity=30.1293900184843, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17550100, Length=507, Percent_Identity=30.9664694280079, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=506, Percent_Identity=30.2371541501976, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71986328, Length=461, Percent_Identity=29.9349240780911, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=516, Percent_Identity=31.3953488372093, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322416, Length=517, Percent_Identity=31.5280464216634, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320788, Length=480, Percent_Identity=28.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322511, Length=347, Percent_Identity=28.2420749279539, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137288, Length=505, Percent_Identity=32.0792079207921, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655642, Length=505, Percent_Identity=32.0792079207921, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655647, Length=505, Percent_Identity=32.0792079207921, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24663005, Length=515, Percent_Identity=29.3203883495146, Blast_Score=168, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56490; Mature: 56490

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDHFRPQSFNGIDDIVIVGGGLAGLFCALKLAPRPVTILAAAPIGYGASSAWAQGGIA
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCHH
AAMSKGDTFDKHVADTVAAGAGIVDEKMTRMMVAEGPARIHDLLEFGVPFDRDLEGKLML
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEE
SREAAHSERRIVRVKGDMAGKSIMEALIAAVRNTPSIRVIEGYVVEELVREGRFISGVVA
EHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEEEEE
RPDAGQSKSRVSFPARAVVLCSGGVGHLYAVTTNPWEACGQGVGMAARAGAIIADPEFVQ
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHCCCCCCHHHCCEEEECCCEEE
FHPTAIDIGKDPAPLATEALRGDGAILINAEGRRFMLDIHPDGELAPRDIVSRGVFAEVQ
EECCEEECCCCCCCHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHCCCEEEEC
AGRGAFLDCTKAVGRHFPDMFPTVYASCMAAGIDPVSQPIPVVPAVHYHMGGVLTDGEGR
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEECCCCC
TSIDGLWAAGEVTSTGVHGANRLASNSLLEAVVFAARIAENIKGSLPAPKLTSWGDNAGE
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NDDPVTVEDSPPFKLLRQLMSDCVGVVRTRESLTRAIQGITRLERANKRLRFANIATTAK
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
LIAVAALQRTESRGGHFRADCPGERPDWQRRTYLTLAQAERLAVEVAESEPA
HHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MLTDHFRPQSFNGIDDIVIVGGGLAGLFCALKLAPRPVTILAAAPIGYGASSAWAQGGIA
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCHH
AAMSKGDTFDKHVADTVAAGAGIVDEKMTRMMVAEGPARIHDLLEFGVPFDRDLEGKLML
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEE
SREAAHSERRIVRVKGDMAGKSIMEALIAAVRNTPSIRVIEGYVVEELVREGRFISGVVA
EHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEEEEE
RPDAGQSKSRVSFPARAVVLCSGGVGHLYAVTTNPWEACGQGVGMAARAGAIIADPEFVQ
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHCCCCCCHHHCCEEEECCCEEE
FHPTAIDIGKDPAPLATEALRGDGAILINAEGRRFMLDIHPDGELAPRDIVSRGVFAEVQ
EECCEEECCCCCCCHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHCCCEEEEC
AGRGAFLDCTKAVGRHFPDMFPTVYASCMAAGIDPVSQPIPVVPAVHYHMGGVLTDGEGR
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEECCCCC
TSIDGLWAAGEVTSTGVHGANRLASNSLLEAVVFAARIAENIKGSLPAPKLTSWGDNAGE
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NDDPVTVEDSPPFKLLRQLMSDCVGVVRTRESLTRAIQGITRLERANKRLRFANIATTAK
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
LIAVAALQRTESRGGHFRADCPGERPDWQRRTYLTLAQAERLAVEVAESEPA
HHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]