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The map label for this gene is cfa [H]

Identifier: 150395909

GI number: 150395909

Start: 736931

End: 738187

Strand: Direct

Name: cfa [H]

Synonym: Smed_0685

Alternate gene names: 150395909

Gene position: 736931-738187 (Clockwise)

Preceding gene: 150395907

Following gene: 150395910

Centisome position: 19.49

GC content: 56.72

Gene sequence:

>1257_bases
ATGAATGCGGGATTGTTGAATCTACTTCGCCGGCTTGTCGAGAAAGGCAAGCTGACGGTCGTTTTCTCGTCGGGTGAAAA
AGTGGTCCTGGGCGATGGTACGGGCAAGCCCGCGGCCATCCGCTTCGTTGATGCCGGAGCGGAGAAAGCCGTCCTGCTCG
ATCCGGGATTGAAATTCGGCGAGATGTACATGGACGGTCGCGTTCTCGTGGAGGAAGGCGACATTTTCGATGTGCTGGCC
ATTGTCAAGAGCAACGATCTCGACAAGGCGGCGACCTTTACTAATTCCATCGTCGGATTGTGGCACATAATGCGTCAGCA
GATGAAGAGCCGCCTGCCGATCAACCGCAACCGCTATAACGTTGCCCATCATTACGATCTGGACGGCAAGCTCTTCAATC
TTTTCCTTGACGAGGACTGGCAATATTCCTGCGCTTACTTTCATCCACCGGGAATTTCTCTCGACGAGGCGCAACGTGCC
AAGAAGAGGCACATAGCGGCCAAGCTGCTGCTCGAGCCCGGCCAGAAGGTATTGGAAGTGGGATCCGGCTGGGGCGGGAT
GGCGATGTATCTCGCCGAGTCTTCCGGGGTCGAAGTCACGGGCATCACGCTCAGCGAAGAGCAGTTGAAAGTTTCGCGCG
AGCGAGCGCAAAGGCGCGGGCTTTCGGACCGCGTGCGCTTCGAGCTGCAGGACTATCGCACCATGCAGGGAAGGCAATTC
GACCGCGTCGTCTCCGTGGGCATGTTCGAACATGTCGGCATCGGCAATTACGGCAATTTTTTCCGTAAGATGAAAGAACT
GCTGAATCCCGATGGAGTGATGCTGCTGCACTCCATCGGGCAGATCTACAAACCCTGGGCAACCAATCCGTGGATCGAAA
AGTACATTTTTCCCGGCGGCTACATTCCCGCCCTGTCCGAGGTGTTGCCTCCCGCCGAGAGCAACCGTCTGCTGGTCAAG
GACATCGAAATACTGCCGATGCATTACGCCTGGACGCTCAGGGCGTGGCGTGAGCGTTTCGTCGCGCAGCGGGAGGAGGC
GATCAGGCTTTATGATGAGCGCTTTTTCCGCATGTGGGAGTTCTATCTCGCCGCCTCCGAAACGGCCTTCCTTTACGACA
AGCACTTCATCTTTCAGCTCCAGCTCTCGCCTTCACTCGCCACCGTGCCGGTATCACGCGATTATATCGCCATCAAGGAG
AGGGAATTGCTGGAGTTCGAAAAGACACGCCAACGGCTGGAGCTCGTCGCGGTCTAA

Upstream 100 bases:

>100_bases
AAAGTATAGCCGTCGCATCTCCGCCATGAAGCCGACCAGCCGCTCACCCGCTCCCCTGTGAACGAATCGAGGGTCCGTTA
GGTCTGAGCTGAGGTAGGAT

Downstream 100 bases:

>100_bases
AGAGGGGCCGGCGAAAAACCCTGAACCGGCCTGCCGGCGACCTTCGCTCCCTTGCGGGGAGAAGGCCGCCGCAGCGGGGT
GAGGCGCGACAGATAGAGAG

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNAGLLNLLRRLVEKGKLTVVFSSGEKVVLGDGTGKPAAIRFVDAGAEKAVLLDPGLKFGEMYMDGRVLVEEGDIFDVLA
IVKSNDLDKAATFTNSIVGLWHIMRQQMKSRLPINRNRYNVAHHYDLDGKLFNLFLDEDWQYSCAYFHPPGISLDEAQRA
KKRHIAAKLLLEPGQKVLEVGSGWGGMAMYLAESSGVEVTGITLSEEQLKVSRERAQRRGLSDRVRFELQDYRTMQGRQF
DRVVSVGMFEHVGIGNYGNFFRKMKELLNPDGVMLLHSIGQIYKPWATNPWIEKYIFPGGYIPALSEVLPPAESNRLLVK
DIEILPMHYAWTLRAWRERFVAQREEAIRLYDERFFRMWEFYLAASETAFLYDKHFIFQLQLSPSLATVPVSRDYIAIKE
RELLEFEKTRQRLELVAV

Sequences:

>Translated_418_residues
MNAGLLNLLRRLVEKGKLTVVFSSGEKVVLGDGTGKPAAIRFVDAGAEKAVLLDPGLKFGEMYMDGRVLVEEGDIFDVLA
IVKSNDLDKAATFTNSIVGLWHIMRQQMKSRLPINRNRYNVAHHYDLDGKLFNLFLDEDWQYSCAYFHPPGISLDEAQRA
KKRHIAAKLLLEPGQKVLEVGSGWGGMAMYLAESSGVEVTGITLSEEQLKVSRERAQRRGLSDRVRFELQDYRTMQGRQF
DRVVSVGMFEHVGIGNYGNFFRKMKELLNPDGVMLLHSIGQIYKPWATNPWIEKYIFPGGYIPALSEVLPPAESNRLLVK
DIEILPMHYAWTLRAWRERFVAQREEAIRLYDERFFRMWEFYLAASETAFLYDKHFIFQLQLSPSLATVPVSRDYIAIKE
RELLEFEKTRQRLELVAV
>Mature_418_residues
MNAGLLNLLRRLVEKGKLTVVFSSGEKVVLGDGTGKPAAIRFVDAGAEKAVLLDPGLKFGEMYMDGRVLVEEGDIFDVLA
IVKSNDLDKAATFTNSIVGLWHIMRQQMKSRLPINRNRYNVAHHYDLDGKLFNLFLDEDWQYSCAYFHPPGISLDEAQRA
KKRHIAAKLLLEPGQKVLEVGSGWGGMAMYLAESSGVEVTGITLSEEQLKVSRERAQRRGLSDRVRFELQDYRTMQGRQF
DRVVSVGMFEHVGIGNYGNFFRKMKELLNPDGVMLLHSIGQIYKPWATNPWIEKYIFPGGYIPALSEVLPPAESNRLLVK
DIEILPMHYAWTLRAWRERFVAQREEAIRLYDERFFRMWEFYLAASETAFLYDKHFIFQLQLSPSLATVPVSRDYIAIKE
RELLEFEKTRQRLELVAV

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=336, Percent_Identity=41.0714285714286, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI133904226, Length=373, Percent_Identity=28.686327077748, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47991; Mature: 47991

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGLLNLLRRLVEKGKLTVVFSSGEKVVLGDGTGKPAAIRFVDAGAEKAVLLDPGLKFG
CCCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCC
EMYMDGRVLVEEGDIFDVLAIVKSNDLDKAATFTNSIVGLWHIMRQQMKSRLPINRNRYN
CEEECCEEEEECCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VAHHYDLDGKLFNLFLDEDWQYSCAYFHPPGISLDEAQRAKKRHIAAKLLLEPGQKVLEV
EEEEECCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSGWGGMAMYLAESSGVEVTGITLSEEQLKVSRERAQRRGLSDRVRFELQDYRTMQGRQF
CCCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCHHHHEEHHHHHHHCCCHH
DRVVSVGMFEHVGIGNYGNFFRKMKELLNPDGVMLLHSIGQIYKPWATNPWIEKYIFPGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCC
YIPALSEVLPPAESNRLLVKDIEILPMHYAWTLRAWRERFVAQREEAIRLYDERFFRMWE
CHHHHHHHCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLAASETAFLYDKHFIFQLQLSPSLATVPVSRDYIAIKERELLEFEKTRQRLELVAV
HHHHCCCEEEEEEEEEEEEEEECCCEEEECCCCCEEEECHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNAGLLNLLRRLVEKGKLTVVFSSGEKVVLGDGTGKPAAIRFVDAGAEKAVLLDPGLKFG
CCCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCC
EMYMDGRVLVEEGDIFDVLAIVKSNDLDKAATFTNSIVGLWHIMRQQMKSRLPINRNRYN
CEEECCEEEEECCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VAHHYDLDGKLFNLFLDEDWQYSCAYFHPPGISLDEAQRAKKRHIAAKLLLEPGQKVLEV
EEEEECCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSGWGGMAMYLAESSGVEVTGITLSEEQLKVSRERAQRRGLSDRVRFELQDYRTMQGRQF
CCCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCHHHHEEHHHHHHHCCCHH
DRVVSVGMFEHVGIGNYGNFFRKMKELLNPDGVMLLHSIGQIYKPWATNPWIEKYIFPGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCC
YIPALSEVLPPAESNRLLVKDIEILPMHYAWTLRAWRERFVAQREEAIRLYDERFFRMWE
CHHHHHHHCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLAASETAFLYDKHFIFQLQLSPSLATVPVSRDYIAIKERELLEFEKTRQRLELVAV
HHHHCCCEEEEEEEEEEEEEEECCCEEEECCCCCEEEECHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]