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The map label for this gene is irlS [H]

Identifier: 150395863

GI number: 150395863

Start: 685009

End: 686406

Strand: Direct

Name: irlS [H]

Synonym: Smed_0639

Alternate gene names: 150395863

Gene position: 685009-686406 (Clockwise)

Preceding gene: 150395862

Following gene: 150395864

Centisome position: 18.11

GC content: 62.37

Gene sequence:

>1398_bases
ATGAGCAGGATCCGCGTGCTGTTCAGGACCACCGCCGTCCGGCTCTCTGCGCTTTATCTGGTCCTCTTCTCCCTCTGCGC
GGCTTTCCTCGTCATTTATGTGACCGGTATGTCGGAGCGCCTCCTGCAGCAGCAGACCCGGGAGGCGATCGCCGCCGAGG
TGAACCAGATCTCGGTTGTCTACGACCGCGCCGGTATCAACGGGCTGCTTCGTTCGCTTGAGCGTCGGGCGCGTCAGCCG
GGAGCGAACCTCTATGTCATTGCCGGGCCGAACGGAGAAATCCTCGCCGGCAATGTCGCCGCTCTGGAGCCGGGGCTACT
CGACGACGAGGGATGGAAGTCGCAGCCGTTCCGCTATCGGCGCTTTACCGACGAGAGTCAGGCAGACAGGCACGTCGCTC
TCGCCCAGGTCCTTGTGCTCGATAACGGCCTGAGACTACTCGTCGGCCGCGATCTGCAGGAGCCGGAGAAGTTCCGCGCG
CTGGTCCGTCAGGCGCTGATGGTAGCGCTCGGCATCATGGGGCTCGGTGCGCTGGTCATCTGGTTCGGTATCGGTCGCAA
TGCTCTGCGCCGCATCGACCGTATGTCGGAAGCGAGCACGAAGATCATGGCCGGGGACCTCTCGCAACGCTTGCCGGCGA
GCGGTTCCGGTGACGAGTTCGACCGCCTTTCGGAATCGCTCAACGCGATGCTGGAACGCATAGAGAAGCTGAATGAAGGA
CTGAAACAGGTTTCCGACAACATCGCGCATGACCTCAAGACACCGCTCACGCGCCTGCGCAACAAGGCAGAAGCGGCACT
TGCAGATGGCGGCGAGGATAGCCGGAGCGGCGCACTCGAGCAGATCATCGCCGAGTCGGATCAGCTGATACGTACGTTCA
ACGCGCTTCTGATGATTTCCCGCGTCGAAGCGGGCTCCGCCGTTGCCGAGATGAGCGAGGTCGACCTGTCCCGGATCGTG
GAGGATTGCGTCGAACTCTATGAGCCGCTGGCGGAAGACAAGGACATGCGCATCGAAGCGGAGGCTGCGCCCGGGGTTGC
GATTAGCGGCAATCGTGAACTCATCAGCCAGGCGTTAGGCAACCTCGTCGACAATGCGATCAAATATGCGGAAGGTGCCG
AAAATCCGCTGATACGGGTTGAAATGAAGCGGAGCGACCGTCAGGTCGTCTTGACTGTCGCCGATCACGGGCCGGGCATT
CCGGAGAATATGCGGGGAGAGGTTGTCAAACGCTTCGTTCGCCTGGACGAAAGCCGATCGAAGCCCGGGACGGGGCTCGG
TCTGTCGCTCGTCGAGGCGATTATGGAGCTGCACCGCGGTTCGCTGGACTTGTCGGCGACGGAGGTGGGTGGCCGAGGCC
TGACGGTTCGCATGGTTTTCCCGGCCGCTGCGACCTGA

Upstream 100 bases:

>100_bases
CGCGCCTGCGTTCGAAGATCGAGAAGGATTTCGATCCACCCTTGCTGAGGACGATCCGTGGTGCCGGATACATGATCAAG
GATGAGCGGGCCGCTGACGC

Downstream 100 bases:

>100_bases
TAGAGCACTTTCAGGAAAAGCGTTTCGTGGTTTCCTGTTTCGGAGGTGCGTCGCCTTAGAGGCACCGGACCGATCGCGGA
GGTGAAGGGGGGAGTGTTCA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSRIRVLFRTTAVRLSALYLVLFSLCAAFLVIYVTGMSERLLQQQTREAIAAEVNQISVVYDRAGINGLLRSLERRARQP
GANLYVIAGPNGEILAGNVAALEPGLLDDEGWKSQPFRYRRFTDESQADRHVALAQVLVLDNGLRLLVGRDLQEPEKFRA
LVRQALMVALGIMGLGALVIWFGIGRNALRRIDRMSEASTKIMAGDLSQRLPASGSGDEFDRLSESLNAMLERIEKLNEG
LKQVSDNIAHDLKTPLTRLRNKAEAALADGGEDSRSGALEQIIAESDQLIRTFNALLMISRVEAGSAVAEMSEVDLSRIV
EDCVELYEPLAEDKDMRIEAEAAPGVAISGNRELISQALGNLVDNAIKYAEGAENPLIRVEMKRSDRQVVLTVADHGPGI
PENMRGEVVKRFVRLDESRSKPGTGLGLSLVEAIMELHRGSLDLSATEVGGRGLTVRMVFPAAAT

Sequences:

>Translated_465_residues
MSRIRVLFRTTAVRLSALYLVLFSLCAAFLVIYVTGMSERLLQQQTREAIAAEVNQISVVYDRAGINGLLRSLERRARQP
GANLYVIAGPNGEILAGNVAALEPGLLDDEGWKSQPFRYRRFTDESQADRHVALAQVLVLDNGLRLLVGRDLQEPEKFRA
LVRQALMVALGIMGLGALVIWFGIGRNALRRIDRMSEASTKIMAGDLSQRLPASGSGDEFDRLSESLNAMLERIEKLNEG
LKQVSDNIAHDLKTPLTRLRNKAEAALADGGEDSRSGALEQIIAESDQLIRTFNALLMISRVEAGSAVAEMSEVDLSRIV
EDCVELYEPLAEDKDMRIEAEAAPGVAISGNRELISQALGNLVDNAIKYAEGAENPLIRVEMKRSDRQVVLTVADHGPGI
PENMRGEVVKRFVRLDESRSKPGTGLGLSLVEAIMELHRGSLDLSATEVGGRGLTVRMVFPAAAT
>Mature_464_residues
SRIRVLFRTTAVRLSALYLVLFSLCAAFLVIYVTGMSERLLQQQTREAIAAEVNQISVVYDRAGINGLLRSLERRARQPG
ANLYVIAGPNGEILAGNVAALEPGLLDDEGWKSQPFRYRRFTDESQADRHVALAQVLVLDNGLRLLVGRDLQEPEKFRAL
VRQALMVALGIMGLGALVIWFGIGRNALRRIDRMSEASTKIMAGDLSQRLPASGSGDEFDRLSESLNAMLERIEKLNEGL
KQVSDNIAHDLKTPLTRLRNKAEAALADGGEDSRSGALEQIIAESDQLIRTFNALLMISRVEAGSAVAEMSEVDLSRIVE
DCVELYEPLAEDKDMRIEAEAAPGVAISGNRELISQALGNLVDNAIKYAEGAENPLIRVEMKRSDRQVVLTVADHGPGIP
ENMRGEVVKRFVRLDESRSKPGTGLGLSLVEAIMELHRGSLDLSATEVGGRGLTVRMVFPAAAT

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=25.4545454545455, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787894, Length=290, Percent_Identity=30.6896551724138, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786783, Length=298, Percent_Identity=25.8389261744966, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=27.6119402985075, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788279, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790551, Length=306, Percent_Identity=27.4509803921569, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789808, Length=310, Percent_Identity=28.0645161290323, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789403, Length=350, Percent_Identity=27.7142857142857, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=28.8990825688073, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=310, Percent_Identity=28.0645161290323, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=28.110599078341, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787374, Length=236, Percent_Identity=24.1525423728814, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=26.25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=222, Percent_Identity=28.8288288288288, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081816, Length=336, Percent_Identity=20.2380952380952, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=24.8945147679325, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=288, Percent_Identity=26.0416666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788713, Length=219, Percent_Identity=21.9178082191781, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50809; Mature: 50678

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIRVLFRTTAVRLSALYLVLFSLCAAFLVIYVTGMSERLLQQQTREAIAAEVNQISVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEE
YDRAGINGLLRSLERRARQPGANLYVIAGPNGEILAGNVAALEPGLLDDEGWKSQPFRYR
EECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHCCCCCCCCCCCCCCCCHHH
RFTDESQADRHVALAQVLVLDNGLRLLVGRDLQEPEKFRALVRQALMVALGIMGLGALVI
HCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WFGIGRNALRRIDRMSEASTKIMAGDLSQRLPASGSGDEFDRLSESLNAMLERIEKLNEG
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKQVSDNIAHDLKTPLTRLRNKAEAALADGGEDSRSGALEQIIAESDQLIRTFNALLMIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVEAGSAVAEMSEVDLSRIVEDCVELYEPLAEDKDMRIEAEAAPGVAISGNRELISQALG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHH
NLVDNAIKYAEGAENPLIRVEMKRSDRQVVLTVADHGPGIPENMRGEVVKRFVRLDESRS
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
KPGTGLGLSLVEAIMELHRGSLDLSATEVGGRGLTVRMVFPAAAT
CCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRIRVLFRTTAVRLSALYLVLFSLCAAFLVIYVTGMSERLLQQQTREAIAAEVNQISVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEE
YDRAGINGLLRSLERRARQPGANLYVIAGPNGEILAGNVAALEPGLLDDEGWKSQPFRYR
EECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHCCCCCCCCCCCCCCCCHHH
RFTDESQADRHVALAQVLVLDNGLRLLVGRDLQEPEKFRALVRQALMVALGIMGLGALVI
HCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WFGIGRNALRRIDRMSEASTKIMAGDLSQRLPASGSGDEFDRLSESLNAMLERIEKLNEG
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKQVSDNIAHDLKTPLTRLRNKAEAALADGGEDSRSGALEQIIAESDQLIRTFNALLMIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVEAGSAVAEMSEVDLSRIVEDCVELYEPLAEDKDMRIEAEAAPGVAISGNRELISQALG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHH
NLVDNAIKYAEGAENPLIRVEMKRSDRQVVLTVADHGPGIPENMRGEVVKRFVRLDESRS
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
KPGTGLGLSLVEAIMELHRGSLDLSATEVGGRGLTVRMVFPAAAT
CCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]