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The map label for this gene is ptnd

Identifier: 15004772

GI number: 15004772

Start: 71378

End: 72289

Strand: Direct

Name: ptnd

Synonym: CA_P0068

Alternate gene names: 15004772

Gene position: 71378-72289 (Clockwise)

Preceding gene: 15004771

Following gene: 15004773

Centisome position: 37.18

GC content: 35.53

Gene sequence:

>912_bases
ATGGCAAATGAATTAAAATTAACAAAGAAGGATCGTATTTCTGTTTGGTGGCGTTCAACTTTCATTCAAGGTTCTTGGAA
CTATGAAAGAATGCAAAACGGTGGTTGGACATTTGCATTAATTCCTGCAATCAAAAAATTATATAAGAATAAAGAAGATC
GTGTAGCTGCATTGAAACGTCACTTAGAGTTTTTTAACACTCACCCATATGTAGCTTCACCAATCATTGGTGTAACATTA
GCCCTAGAAGAAGAACGTGCAAATGGTGCACCAGTTGATGATGTAACTATTCAAGGTGTTAAAGTTGGTATGATGGGACC
TTTAGCAGGTATCGGAGATCCAGTTTTCTGGTTTACTGTTAAGCCAATTTTAGGAGCATTAGCAGCTTCACTTGCTATGA
GTGGTAACATTCTTGGACCATTTATATATTTCTTTGCTTGGAATGCCATCCGTATGTTATTTATGTGGTATACACAAGAG
TTTGGTTACAAAGCAGGCTCTCGTATTACCGATGATCTATCAGGTGGTTTACTACAAGACGTTACAAAAGGAGCATCCAT
CCTTGGTATGTTTATTTTGGGATCGTTAGTTAATAGATGGGTATCTGTTAAATTTACACCGGTAGTATCCTCTGTTAAGT
TAAGTGATGGTGCATATATTGTTTGGGATAAACTTCCGGCTGGAGTTAAAGGTATTAAAGAAGCTTTGATTCAGCAGTCA
TCTGGTTTGTCGTTAACGGATCATAAAGTTACAACACTACAAAATAACCTGGATTCATTAATTCCTGGACTTGCAGGATT
AGCAGTAACATTTATTTGTATGTGGTTACTTAAGAAGAAAGTATCTCCAATTGTTATTATTTTTGGTTTATTCATAATCG
GTATTGTTTTCCATTTAATTGGTTTAATGTAA

Upstream 100 bases:

>100_bases
CTTTCTAAACAAGGCGGCTCAGGTAAGGGTGGTGGATCAAATACTGGTGATCCATTGGGCGATATTATCGATAGATACTA
AGAAAGGAGAGAACACGAAA

Downstream 100 bases:

>100_bases
TATTTCTTACTTAGCCTGGGCTTTGGCTAAACATATTATTGATTTTAATTTAATATGTTTAACTTAAGCCTAGGCTTTTG
TTAAGAAATAGATTGAGAAA

Product: mannose-specific phosphotransferase system component IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MANELKLTKKDRISVWWRSTFIQGSWNYERMQNGGWTFALIPAIKKLYKNKEDRVAALKRHLEFFNTHPYVASPIIGVTL
ALEEERANGAPVDDVTIQGVKVGMMGPLAGIGDPVFWFTVKPILGALAASLAMSGNILGPFIYFFAWNAIRMLFMWYTQE
FGYKAGSRITDDLSGGLLQDVTKGASILGMFILGSLVNRWVSVKFTPVVSSVKLSDGAYIVWDKLPAGVKGIKEALIQQS
SGLSLTDHKVTTLQNNLDSLIPGLAGLAVTFICMWLLKKKVSPIVIIFGLFIIGIVFHLIGLM

Sequences:

>Translated_303_residues
MANELKLTKKDRISVWWRSTFIQGSWNYERMQNGGWTFALIPAIKKLYKNKEDRVAALKRHLEFFNTHPYVASPIIGVTL
ALEEERANGAPVDDVTIQGVKVGMMGPLAGIGDPVFWFTVKPILGALAASLAMSGNILGPFIYFFAWNAIRMLFMWYTQE
FGYKAGSRITDDLSGGLLQDVTKGASILGMFILGSLVNRWVSVKFTPVVSSVKLSDGAYIVWDKLPAGVKGIKEALIQQS
SGLSLTDHKVTTLQNNLDSLIPGLAGLAVTFICMWLLKKKVSPIVIIFGLFIIGIVFHLIGLM
>Mature_302_residues
ANELKLTKKDRISVWWRSTFIQGSWNYERMQNGGWTFALIPAIKKLYKNKEDRVAALKRHLEFFNTHPYVASPIIGVTLA
LEEERANGAPVDDVTIQGVKVGMMGPLAGIGDPVFWFTVKPILGALAASLAMSGNILGPFIYFFAWNAIRMLFMWYTQEF
GYKAGSRITDDLSGGLLQDVTKGASILGMFILGSLVNRWVSVKFTPVVSSVKLSDGAYIVWDKLPAGVKGIKEALIQQSS
GLSLTDHKVTTLQNNLDSLIPGLAGLAVTFICMWLLKKKVSPIVIIFGLFIIGIVFHLIGLM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=302, Percent_Identity=48.6754966887417, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1789529, Length=300, Percent_Identity=32.3333333333333, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 33430; Mature: 33299

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANELKLTKKDRISVWWRSTFIQGSWNYERMQNGGWTFALIPAIKKLYKNKEDRVAALKR
CCCCCCCCCCCCEEEEEEHEEEECCCCHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHH
HLEFFNTHPYVASPIIGVTLALEEERANGAPVDDVTIQGVKVGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHCCHHHEEEEEECCCCCCCCCCCEEECCEEEECCCCCCCCCCCHHHHHH
KPILGALAASLAMSGNILGPFIYFFAWNAIRMLFMWYTQEFGYKAGSRITDDLSGGLLQD
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHH
VTKGASILGMFILGSLVNRWVSVKFTPVVSSVKLSDGAYIVWDKLPAGVKGIKEALIQQS
HHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCC
SGLSLTDHKVTTLQNNLDSLIPGLAGLAVTFICMWLLKKKVSPIVIIFGLFIIGIVFHLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GLM
HCC
>Mature Secondary Structure 
ANELKLTKKDRISVWWRSTFIQGSWNYERMQNGGWTFALIPAIKKLYKNKEDRVAALKR
CCCCCCCCCCCEEEEEEHEEEECCCCHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHH
HLEFFNTHPYVASPIIGVTLALEEERANGAPVDDVTIQGVKVGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHCCHHHEEEEEECCCCCCCCCCCEEECCEEEECCCCCCCCCCCHHHHHH
KPILGALAASLAMSGNILGPFIYFFAWNAIRMLFMWYTQEFGYKAGSRITDDLSGGLLQD
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHH
VTKGASILGMFILGSLVNRWVSVKFTPVVSSVKLSDGAYIVWDKLPAGVKGIKEALIQQS
HHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCC
SGLSLTDHKVTTLQNNLDSLIPGLAGLAVTFICMWLLKKKVSPIVIIFGLFIIGIVFHLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GLM
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]